한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제31권3호
  • /
  • Pages.369-374
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  • 2008
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지

MC1R gene의 PCR-RFLP를 이용한 한우.젖소고기 감별

Analysis of Melanocortin receptor 1 (MC1R) gene differential test for beef species between Hanwoo and Holstein using polmerase chain reaction -restriction fragment length polymorphism (PCR-RFLP)

  • 서동균 (대구광역시 보건환경연구원)
  • Suh, Dong-Kyun (Research Institute of Health & Environment, Daegu Metropolitan City)
  • 발행 : 2008.09.30
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초록

The objective of this study was to differentiate the beef species between Hanwoo and Holstein from a total of 1,081 beef samples using PCR-RFLP of MC1R gene. When a PCR product of 403 bp specific band amplified from bovine MC1R gene sequence was digested with restriction enzyme MspA1I, Hanwoo type showed 2 bands, 220 bp and 183 bp size bands. Holstein type, however, showed three bands, 220 bp, 138 bp and 45 bp size band, respectively. The results of the differential test for beef species were as following; 7 samples (0.64%) were determined to Holstein type, of which 4 were submitted from administrative authorities, other 3 from self-collection planing, and none from civilian clients including school.

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참고문헌

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