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연근 추출물의 항산화 효과

Antioxidaive Activity of Lotus Root(Nelumbo nucifera G.) Extracts.

  • 이재준 (조선대학교 식품영양학과) ;
  • 하진옥 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과)
  • 발행 : 2007.09.30

초록

본 연구에서는 연근의 유용자원으로의 이용 가능성을 알아보기 위하여, 연근 추출물을 제조하여 in vitro 에서 합성항산화제로 널리 알려진 BHT와의 비교 측정하기 위하여 연근 에탄올 추출물의 각 분획별 항산화력 활성을 검토하였다. 연근 분획물의 항산화성을 알아보기 위하여 DPPH radical에 대한 자유기 소거능, Rancimat에 의한 항산화지수, 아질산염 소거능, 지질과산화물 생성 억제효과 등을 측정하였고, 총 polyphenol 함량을 측정하였다. 그 결과 연근 에탄올 추출물의 수율은 9.14% 이었고, 연근 에탄올 추출물의 계통 분획한 수율은 n-butanol이 가장 높았으며, 다음으로는 water, eth-ylacetate, chloroform, n-hexane 순이었다. 연근 에탄올 추출물의 총 polyphenol 함량은 0.306 mg/ml이었고, 각 분획물 중 ethylacetate 분획물이 0.843 mg/ml로 가장 많이 함유된 것으로 나타났다. DPPH radical에 대한 소거능을 측정한 결과 ethylacetate 분획물이 15.69 ${\mu}g/ ml$로 가장 강한 소거능을 나타내었으며, 합성항산화제 BHT의 15.38 ${\mu}g$/ml 와 유사한 효과를 나타내었다. Rancimat의 지질 산패도 유도기간에 따른 항산화력 비교에서도, ethylacetate 분획물이 가장 길었으나 양성대조군인 BHT의 유도기간에 비하여 짧게 나타났다. 아질산염 소거능은 pH 1.2에서 가장 우수하였으며, pH 1.2에서 ethylacetate 분획물이 88.33%로 합성항산화제인 BHT와 비슷한 아질산염 소거능을 나타내었다. 지질과산화물 생성 억제효과도 ethylacetate 분획물이 87.23%로 가장 우수하였다. 전체적으로 연근 ethylacetate 분획물은 BHT와 유사한 항산화 활성을 나타내었다. 연근 추출물은 in vitro 항산화 실험에서 항산화 활성을 나타내는 생리활성 성분이 존재하는 것으로 볼 수 있으므로 천연 항산화제로서의 가능성을 시사하였다.

This study was investigated on the antioxidant capacity of 80% ethanol extract of lotus root in vitro. The ex-traction yields of 80 % ethanol extract was 9.14%. Lotus root ethanol extract was fractionated by the following: n-hexane, chloroform, ethylacetate and n-butanol. n-Butanol fraction showed the highest ex-traction yield of all fractions. Antioxidative activities of different fractions were examined by l.l-diphenyl-2-picrylhydrazyl(DPPH) radical generation, the Rancimat test, the nitrite scavenging ac-tivity and the thiobarbituric acid(TBA) method, and compared with the properties of the commercial antioxidant butylated hydroxytoluene(BHT). The antioxidative capacity of the ethylacetate fraction was the highest among fractions and its fraction showed higher contents of total polyphenol. Furthermore, the antioxidative capacity of the ethylacetate fraction was similar to that of BHT. In conclusion, these results suggest that lotus root may be a good candidate as a natural antioxidant source.

키워드

참고문헌

  1. Antolovich, M., P. D. Prenzler, E. Patsalides, S. McDonald and K. Robards. 2003. Methods for testing antioxidant activity. Analyst 127, 183-198 https://doi.org/10.1039/b009171p
  2. A.O.A.C. 1990. Official Methods of Analysis. pp. 8-15. 15th ed. Association of official analytical chemists. Washington DC
  3. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1200
  4. Borsch, T. and W. Barthlott. 1983. Classification and distribution of the genus Nelumbo adans (Nelumbonaccae). Beitr. Biol. Plazen 68, 421-450
  5. Borrello, S., A. Seccia, T. Galleotti, G. M. Bartoli, E. Farallo and F. Serri, 1984. Protective enzymes in human epidermal carcinomas and psoriasis. Arch. Dermatol. Res. 276, 338-340 https://doi.org/10.1007/BF00404630
  6. Cha, G. S. and C. U. Choi, 1990. Determination of oxidation stability of perilla oil by the Rancimat method. Korean J. Food Sci. Technol. 22, 61-65
  7. Cho, E. J., T. Yokozawa, D. Y. Rhyu, S. C. Kim, N. Shibahara and J. C. Park. 2003. Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1-diphenyl-2-picrylhydrazyl radical. Phytomedicine 10, 544-551 https://doi.org/10.1078/094471103322331520
  8. Cho, S. H, R. K. Kang and H. G. Lee. 1984. A study on the ingredients preparation method of lotus root jung kwa. J. Korean Soc. Food Sci. Nutr. 13, 42-50
  9. Cho, S. I. and H. W. Kim. 2003. Beneficial effect of Nodus neoumbinis nhizomatis extracts on cisplatin-induced kidney toxicity in rats. Korean J. Herbology 18, 127-134
  10. Chung, K. S., H. S. Yun-Choi and Y. Hahn. 2000. High performance liquid chromatographic analyses of higenamine enatiomers in aconite roots. Natural Product Sci. 6, 20-24
  11. Esquivel, M. M., M. A. Ribeiro and M. G. BermardoGil. 1999. Supercritical extraction of savory oil : study of antioxidant activity and extract characterization. J. Super critical Fluids 14, 129-138 https://doi.org/10.1016/S0896-8446(98)00115-6
  12. Gray, J. I. and J. R. Dugan. 1975. Inhibition of n-nitrosamine formation in model food systems. J. Food Sci. 40, 981-984 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  13. Han, S. J. and S. J. Koo. 1993. Study of the chemical composition in bamboo, shoot, lotus root and burdock. Korean J. Soc. Food Sci. 9, 82-87
  14. Harman, D. 1987. The free radical theory of aging. In modern biological theories of aging. pp. 89. Warner, H. R., R. N. Butler, R. L. Sprott, E. L. Schneider, eds. Raven Press NY
  15. Hong, J. Y., H. S. Nam, Y. S. Lee, K. Y. Yoon, N. W. Kim and S. R. Shin. 2006. Study on the antioxidant activity of extracts from the fruit of Elaeagnus multiflora thunb. Korean J. Food Reserv. 13, 413-419
  16. Hu, M. H and L. H. Skibsted. 2002. Antioxidative capacity of rhizome extract and rhizome knot extract of edible lotus(Nelumbo Nucifera). Food Chem. 76, 327-333 https://doi.org/10.1016/S0308-8146(01)00280-1
  17. Hur, S. K., S. S. Kim, Y. H. Heo, S. M. Ahn, B. G. Lee and S. K. Lee. 2001. Effect of the grapevine shoot extract on free radical scavenger activity and inhibition of pro-inflammatory mediator production in raw macrophages. J. Applied Pharmacol. 9, 188-193
  18. Jae, J. U. 1989. Isolation and identification of constituents from antioxidant and hematopoietic fractions of panax ginseng CA. Mayer. Ph D Thesis. Seoul University
  19. Joo, K. L. and J. J. Kim. 2002. Oxidative stability and compounds of sesame oils blended with vegetable oils. Korean J. Food Sci. Technol. 34, 499-502
  20. Jung, G. T., I. O. Ju, J. S. Choi and J. S. Hong. 2000. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT(Omija) seed. Korean J. Food Sci. Technol. 32, 928-935
  21. Jung, H. A., J. E. Kim, H. Y. Chung and J. S. Choi. 2003. Antioxidant principles of Nelumbo nucifera stamens. Arch. Pharm. Res. 26, 279-285 https://doi.org/10.1007/BF02976956
  22. Kang, Y. H, Y. K. Park, S. R. Oh and K. D. Moon. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J. Food Sci. Technol. 27, 978-984
  23. Kang, Y. H, Y. K. Park and G. D. Lee. 1996. The nitrite scavenging and electron donating ability of polyphenolic compounds(in Korean). Korean J. Food Sci. Technol. 28, 232-239
  24. Kato, H., C. Lee, N. V. Kim and S. B. Hayase. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins, Agric. Bio. Chem. 51, 1333-1338 https://doi.org/10.1271/bbb1961.51.1333
  25. Kim, J. S. 2002. Anti-diabetic constituent from the node of lotus rihzome(Nelumbo nucifera Gaertn). MS Thesis. ChungAng University
  26. Kim, S. B., S. B. Rho, D. Y. Rhyu and D. W. Kim. 2005. Effect of Nelumbo nucifera leaves on hylerlipidemic and atherosclerotic bio F1B hamster. Korean J. Pharmacogn. 36, 229-234
  27. Kim, Y. S., S. S. Jeon and S. T. Jung. 2002. Effect of lotus powder on the baking quality of white bread. Korean J. Soc. Food Cookery Sci. 18, 413-425
  28. Ko, B. S., D. W. Jun, J. S. Jang, J. H. Kim and S. Park. 2006. Effect of Sasa Borealis and white lotus roots and leaves on insulin action and secretion in vivo. Korean J. Food Sci. Technol. 38, 114-120
  29. Kytopoulos, S. A. 1987. Ascorbic acid and formation of n-nitroso compounds: possible role of ascorbic acid in cancer prevention. Am. J. Clin. Nutr. 45, 1344-1350 https://doi.org/10.1093/ajcn/45.5.1344
  30. Lee, J. J., S. E. Park and M. Y. Lee. 2006. Effects of lotus root(Nelumbo nucifera G.) on lipid metabolism in rats with diet-induced hypercholesterolemia. Korean J. Food Preserv. 13, 634-642
  31. Lee, J. J., S. E. Park, Y. M. Lee and M. Y. Lee. 2006. Protective effects of lotus root(Nelumbo nucifera G.) on hepatic injury induced by alcohol in rats. Korean J. Food Preserv. 13, 774-782
  32. Lee, K. D., H. K. Chang and H. K. Kim. 1997. Antioxidative and nitrite scavenging activities of edible mushrooms. Korean J. Food Sci. Technol. 29, 432-436
  33. Lee, K. S., M. G Kim and K. Y. Lee. 2004. Antimicrobial effect of the extracts of cactus Chounnyouncho(Opuntia humifusa) aganist food borne pathogens. J. Korean Soc. Food Sci. Nutr. 33, 1268-1272 https://doi.org/10.3746/jkfn.2004.33.8.1268
  34. Lee, K. S., M. G. Kim and K. Y. Lee. 2006. Antioxidative activity of ethanol extract from lotus(Nelumbo nucifera) leaf. J. Korean Soc. Food Sci. Nutr. 35, 182-185 https://doi.org/10.3746/jkfn.2006.35.2.182
  35. Lee, M. W., J. S. Kim, S. M. Cho, J. H. Kim and J. S. Lee. 2001. Anti-diabetic constituent from the node of lotus rhizome(Nelumbo nucifera Gaertn). Natural Product Sci. 7, 107-109
  36. Ling, Z. Q., B. J. Xie and E. L. Yang. 2005. Isolation, characterization, and determination of antioxidative of oligomeric procyanidins from the seedpod of Nelumbo nucifera Gaertn. J. Agric. Food Chem. 53, 2441-2445 https://doi.org/10.1021/jf040325p
  37. Moon, S. M., H. J. Kim and K. S. Ham. 2003. Purification and characterization of polyphenol oxidase from lotus root(Nelumbo nucifera G). Korean J. Food Sci. Technol. 35, 791-796
  38. Na, M. K., R. N. An, S. M. Lee, N. D. Hong, J. K. Yoo, C. B. Lee, J. P. Kim and K. H. Bae. Screening of crude drugs for antioxidative activity. Kor. J. Phannacogn. 32, 108-115
  39. Ottolenghi, A. 1959. Interaction of ascorbic acid and mitochondrial lipids. Arch. Biochem. Biophy. 79, 355-461 https://doi.org/10.1016/0003-9861(59)90414-X
  40. Paker, L. and A. N. Glazer. 1990. Oxygen radicals in biological systems. In Methods in Enzymology. Oxygen radicals in biological systems. pp. 186-343. Academic Press, London
  41. Park, I. B., J. W. Park, J. M. Kim, S. T. Jung and S. G. Kang. 2005. Quality of soybean paste(Doenjang) prepared with lotus root powder. J. Korean Soc. Food Sci. Nutr. 34, 519-523 https://doi.org/10.3746/jkfn.2005.34.4.519
  42. Park, S. H, E. H. Shin, J. G. Koo, T. H. Lee and J. H. Han. 2005. Effects of Nelumbo nucifera on the regional cerebral blood flow and blood pressure in rats. J. East Asian Soc. Dietary Life 15, 49-56
  43. Park, S. H., T. S. Hahm and J. H Han. 2005. Effects of ethanol-extract of lotus root on the renal function and blood pressure of fructose-induced hypertensive rats. J. East Asian Soc. Dietary Life 15, 165-170
  44. Park, S. H, J. S. Hyun, E. H. Shin and J. H. Han. 2005. Functional evaluation of lotus root on serum lipid profile and health improvement. J. East Asian Soc. Dietary Life 15, 257-263
  45. Quan, J. Q. 2002. Cardiovascular pharmacological effects of bibenzylisoquinoline alkaloid derivatives. Acta. Pharmacol. Sin. 23, 1086-1092
  46. Sang, D., J. H. Kim and J. O. Hoon. 1981. Antioxidant activity of panax ginseng browning products. J. Korean Agri. Chem. Soc. 24, 161-166
  47. Won, H. T. 2004. Effects of Nodus neoumbinis nhizomatis extracts on sociopsychological stress in mice. MS Thesis. Dongshin University
  48. Yang, H. C, Y. H. Kim, T. K. Lee and Y. S. Cha. 1985. Physicochemical properties of lotus root(Nelumbo nucifera G.) starch. J. Korean Agri. Chem. Soc. 28, 239-244
  49. Xiao, J. H., J. H. Zhang., H. L. Chen., X. L. Feng and J. L. Wang. 2005. Inhibitory effects of isoliensinine on bleomycin-induced pulmonary fibrosis in mice. Planta Med. 71, 225-227 https://doi.org/10.1055/s-2005-837821

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