Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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The Effects of Growth Inhibition and Quinone Reductase Activity Stimulation of Celastrus Orbiculatus Fractions in Various Cancer Cells

노박덩굴 분획물의 암세포 증식 억제 효과 및 Quinone Reductase 활성 증가효과

  • Ku, Mi-Jeong (Department of Food and Nutrition, Silla University) ;
  • Shin, Mi-Ok (Department of Food and Nutrition, Silla University)
  • 구미정 (신라대학교 식품영양학과) ;
  • 신미옥 (신라대학교 식품영양학과)
  • Published : 2007.09.30
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Abstract

Celastrus orbiculatus (CO) has been used as a traditional herb medicine to treat fever, chill, joint pain, edema, rheumatoid arthritis and bacterial infection in China and Korea. In this study, we investigated anticarcinogenic effects of Celastrus orbiculatus (CO). CO was extracted with methanol (COM), and then further fractionated into four different types: methanol (COMM), hexane (COMH), butanol (COMB) and aqueous (COMA) partition layers. We determined the cytotoxicity of these four partitions in four kind of cancer cell lines, such as HepG2, MCF-7, HT29 and B16F10 Cells by MTT assay. Among various partition layers of CO, the COMM showed the strongest cytotoxic effects on cancer cell lines we used. We also observed quinone reductase (QR) induced effects in all partition layers of CO on HepG2 cells. The QR induced effects of COMM on HepG2 cells at 80 ${\mu}$ g/mL concentration indicated 3.28 to a control value of 1.0. The COMM showed the highest induction activity of quinone reductase on HepG2 cells among the other partition layers. Although further studies are needed, the present work suggests that CO may be a chemopreventive agent for the treatment of human cells.

Keywords

References

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