Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Development of Biologically Active Compounds from Edible Plant Sources-XIX. Isolation of Inhibitory Compound on LDL-Oxidation from the Aerial Parts of Sajabalssuk (Artemisia princeps PAMPANINI, Sajabalssuk)

식용식물자원으로부터 활성물질의 탐색-XIX. 사자발쑥(Artemisia princeps PAMPANINI, Sajabalssuk)의 전초로부터 저밀도 지질 단백질(LDL) 산화 억제물질의 분리

  • Bang, Myun-Ho (Ganghwa Agricultural R&D Center) ;
  • Song, Myoung-Chong (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Han, Min-Woo (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Dae-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Jo, Jin-Kyung (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Chung, Hae-Gon (Ganghwa Agricultural R&D Center) ;
  • Jeong, Tae-Sook (National Research Laboratory of Lipid Metabolism & Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Kyung-Tae (Department of Biochemistry, College of Pharmacy, Kyung Hee University) ;
  • Choi, Myung-Sook (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
  • 방면호 (강화군 특화작목연구소) ;
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 한민우 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이대영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 조진경 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 정해곤 (강화군 특화작목연구소) ;
  • 정태숙 (한국생명공학연구원 지질대사연구실) ;
  • 이경태 (경희대학교 약학대학 약품생화학교실) ;
  • 최명숙 (경북대학교 식품영양학과) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2007.09.30
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Abstract

Sajabalssuk (Artemisia princeps PAMPANINI) was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_{2}O$, successively. From the EtOAc fraction, two phenylpropanoids were isolated through the repeated silica gel and ODS column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the phenylpropanoids were determined as eugenol (1) and (-)-sesamin (2). They were the first to be isolated from Sajabalssuk (Artemisia princeps PAMPANINI, Sajabalssuk). Also, eugenol inhibited LDL-oxidation with the inhibitory activity of 87.8 ${\pm}$ 1.0% at a concentration of 40 ${\mu}g/ml$.

사자발쑥의 전초를 80% MeOH 용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS)column chromatography로 정제하여 2종의 phenylpropanoids 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR등의 스펙트럼 데이터를 해석하여 eugenol(1) 및 (-)-sesamin(2)으로 동정하였다. 이 화합물들은 사자발쑥에서 처음 분리되었다. Eugenol(1)은 LDL-항산화활성 억제활성이 40 ${\mu}g/ml$ 처리 농도에서 $87.8{\pm}1.0%$로 나타났다.

Keywords

References

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