Volatile Compounds and Antioxidant Activities of Adenophora remotiflora

모시대(Adenophora remotiflora) 추출물의 휘발성 성분 및 항산화 활성

  • Kim, Sung-Hyang (Department of Food and Nutrition, Duksung Women's University) ;
  • Choi, Hyang-Sook (Department of Food and Nutrition, Duksung Women's University) ;
  • Lee, Mie-Soon (Department of Food and Nutrition, Duksung Women's University) ;
  • Chung, Mi-Sook (Department of General Education, Duksung Women's University)
  • 김성향 (덕성여자대학교 자연과학대학 식품영양학과) ;
  • 최향숙 (덕성여자대학교 자연과학대학 식품영양학과) ;
  • 이미순 (덕성여자대학교 자연과학대학 식품영양학과) ;
  • 정미숙 (덕성여자대학교 교양교직대학 식품학)
  • Published : 2007.04.30

Abstract

Adenophora remotiflora (Mosidae) is a perennial plant in the Campanulaceae family and a wild plant that only inhabits in Korea. This research analyzed the volatile compounds in Mosidae and their antioxidant activities. The volatile compounds in fresh, shady air-dried, and freeze-dried Mosidae were isolated by steam-distillation extraction (SDE) method using diethylether as a solvent. Volatile compounds were analyzed by gas chromatography-mass spectrometry (GC/MS). Antioxidant activities were determined using the linoleic acid system and 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Sixty and seventy-two volatile compounds were identified from fresh and shady air-dried Mosidae, respectively. In fresh Mosidae, the most abundant compounds were ethyl acetate and heptyl acetate, while ethyl acetate and limonene were the most abundant in the shady air-dried sample. Inhibition of peroxide formation by fresh Mosidae was higher than that of ${\alpha}-tocopherol$, and inhibition by shady air-dried Mosidae was same as that of ${\alpha}-tocopherol$. Furthermore, volatile compounds from shady air-dried Mosidae had higher free radical scavenging activity than ${\alpha}-tocopherol$. The freeze-dried sample showed lower antioxidant activity in both the linoleic acid system and DPPH method.

신선 및 자연건조 모시대의 휘발성 성분을 SDE 방법으로 추출한 후 GC 및 GC/MS를 이용하여 분석하였으며, 모시대의 휘발성 성분 추출물을 시료로 하여 linoleic acid system과 DPPH 방법으로 유리라디칼 소거 활성을 측정하였다. 신선한 모시대에서 산류 4종, 알데하이드류 7종, 알코올류 12종, 에스터류 9종, 탄화수소류 23종, 케톤류 2종 및 기타 3종의 총 60종 휘발성 성분이 확인되었다. 자연건조한 모시대에서 확인된 성분은 총 72종으로 산류 6종, 알데하이드류 11종, 알코올류 14종, 에스터류 10종, 탄화수소류 25종, 케톤류 3종 및 기타 3종이었다. Linoleic acid peroxidation 억제효과를 살펴보면, BHT가 가장 높은 억제 효과가 나타났고 ${\alpha}-tocopherol$과 비교하였을 때 신선한 모시대 추출물의 휘발성 성분의 억제효과가 높았으며, 자연건조 시료는 동일하였고 동결건조 시료는 낮게 나타났다. 또한 모시대 휘발성 성분 추출물의 항산화 활성을 DPPH로 측정한 결과, BHT가 가장 높았으며 자연건조한 모시대의 휘발성 성분이 ${\alpha}-tocopherol$ 보다 높은 유리라디칼 소거활성을 보였으며 신선한 모시대의 휘발성 성분도 ${\alpha}-tocopherol$과 같은 수준의 활성을 나타내었고 동결건조 시료는 낮게 나타났다.

Keywords

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