Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Regulation of β-xylosidase biosynthesis in Paenibacillus sp. DG-22

Paenibacillus sp. DG-22에서의 β-xylosidase 생합성 조절

  • Lee, Tae-Hyeong (Department of Biotechnology, Dongguk University) ;
  • Lim, Pyung-Ok (Department of Science Education, Cheju National University) ;
  • Lee, Yong-Eok (Department of Biotechnology, Dongguk University)
  • 이태형 (동국대학교 과학기술대학 생명공학과) ;
  • 임평옥 (제주대학교 사범대학 과학교육과) ;
  • 이용억 (동국대학교 과학기술대학 생명공학과)
  • Published : 2007.03.30
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Abstract

Regulation of ${\beta}-xylosidase$ synthesis in Paenibacillus sp. DC-22 was studied to optimize the enzyme production. ${\beta}-Xylosidase$ synthesis of the Paenibacillus sp. DG-22 was observed to be regulated by carbon sources present in culture media. The synthesis of ${\beta}-xylosidase$ was induced by xylan and methyl ${\beta}-D-xylopyranoside$ (${\beta}MeXyl$) but slightly repressed by readily metabolizable monosaccharides. ${\beta}MeXyl$ was found to be the best substrate for the induction of ${\beta}$-xylosidase and the most effective induction was obtained at a concentration of 10 mg/ml. ${\beta}-Xylosidase$ production showed a cell growth associated profile with the maximum amount formed during the late exponential phase of growth. The presence of glucose and xylose decreased the level of ${\beta}-xylosidase$ activity indicating that its production was subjected to a form of carbon catabolite repression. SDS-PAGE and zymogram techniques demonstrated the induction by ${\beta}MeXyl$ and revealed the presence of one ${\beta}-xylosidase$ of approximately 80 kDa.

효소생산을 최적화하기 위해서 Paenibacillus sp. DG-22에서의 ${\beta}-xylosidase$ 생합성 조절을 연구하였다. Paenibacillus sp. DG-22의 ${\beta}-xylosidase$는 배양액에 존재하는 탄소원에 의해 조절되는 것으로 관찰되었다. ${\beta}-Xylosidase$의 합성은 xylan과 methyl ${\beta}-D-xylopyranoside$ (${\beta}MeXyl$)에 의해 유도되었으나 쉽게 대사되는 단당류에 의해서는 약간 억제되었다. ${\beta}MeXyl$가 ${\beta}-xylosidase$의 유도를 위한 최적의 기질임을 확인하였고 가장 효과적인 유도는 10 mg/ml의 농도에서 얻어졌다. ${\beta}-Xylosidase$의 생산은 세포의 생장과 연관된 양상을 나타내었으며, 대수기 말에 최대양이 형성되었다. Glucose와 xylose가 존재하면 ${\beta}-xylosidase$의 활성 수준이 감소하는 것으로 보아 이 효소의 생합성은 catabolite repression을 받는것으로 보인다. SDS-PAGE와 활성염색 기술을 이용하여 ${\beta}Mexyl$가 이 효소의 생합성을 유도하며 약 80 kDa 크기의 하나의 ${\beta}-xylosidase$가 존재함을 알 수 있었다.

Keywords

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