Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Antinociceptive Efficacy of Korean Bee Venom in the Abdominal Pain of the Mouse

마우스 복통에 대한 한국산 봉독의 진통 효과

  • Kim, Joong-Hyun (College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Hye-Yun (College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Myoung-Hwan (College of Veterinary Medicine, Chungbuk National University) ;
  • Han, Tae-Sung (College of Veterinary Medicine, Chungbuk National University) ;
  • Cho, Ki-Rae (College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Gon-Hyung (College of Veterinary Medicine, Chungbuk National University) ;
  • Choi, Seok-Hwa (College of Veterinary Medicine, Chungbuk National University)
  • 김중현 (충북대학교 수의과대학, BK21 동물의료생명과학사업단) ;
  • 이혜윤 (충북대학교 수의과대학, BK21 동물의료생명과학사업단) ;
  • 김명환 (충북대학교 수의과대학, BK21 동물의료생명과학사업단) ;
  • 한태성 (충북대학교 수의과대학, BK21 동물의료생명과학사업단) ;
  • 조기래 (충북대학교 수의과대학, BK21 동물의료생명과학사업단) ;
  • 김근형 (충북대학교 수의과대학, BK21 동물의료생명과학사업단) ;
  • 최석화 (충북대학교 수의과대학, BK21 동물의료생명과학사업단)
  • Published : 2007.09.30
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Abstract

This study was undertaken to assess the antinociceptive effect of Korean bee venom (BV) in mice. Korean BV was collected using BV collector devices in which an electrical impulse is used to stimulate the worker bee (Apis mellifera L.) to sting and release venom. After collection, whole BV was evaporated until dry using the BV collector. Experiments were performed on male ICR mice (weighing $30{\sim}35g$, 6 weeks old). Mice were divided into 4 groups, each comprising 8 mice. BV was diluted and amounts of 6 mg/kg body weight (BW), 0.6 mg/kg BW and 0.06 mg/kg BW were tested. BV was subcutaneously injected to produce an antinociceptive effect and the antinociceptive efficacy was evaluated using a writhing test in mice. Intraperitoneal injection of acetic acid produced a tonic pain behavior, first observed at 3 to 9 min post-injection. This writhing response peaked at 20 min post-acetic acid injection, and then declined until it was undetectable at 60 min post-injection. The time elapse between the administration of acetic acid and the first observed pain behaviors indicated a dose-dependent suppressive effect. These results suggest that Korean BV may be used to achieve an antinociceptive effect for use in medical therapies.

본 연구에서는 마우스의 복부 신전시험을 통해 한국산 건조 봉독의 진통 효과를 평가하였다. 한국산 봉독은 특별히 고안된 봉독 추출기를 사용하여 일벌 (Apis mellifera L.)에 전기 충격을 가하여 생봉독을 수집하였으며, 수집된 생봉독은 봉독 건조기를 이용하여 봉독을 건조하였다. 실험 동물은 수컷 ICR 마우스 (체중 30-35 g, 6주령) 56마리를 각 군당 8마리씩 4개군으로 분류하였다. 건조 봉독은 6 mg/kg 투여군, 0.6 mg/kg 투여군, 0.6 mg/kg을 투여군 및 생리식염수를 투여한 대조군으로 각각 분류하였다. 한국산 건조 봉독은 마우스에 피하로 투여하였고, 봉독투여 30분후에는 0.9% 초산을 복강으로 투여하였다. 마우스의 복부의 신전 반사 횟수는 초산 투여 후 60분 동안 측정하였다. 초산 투여 후 3-9분에는 복부신전 반사를 보였고, 20분 후에는 가장 심한 복부신전 반사가 있었고 60분 후에는 반응이 거의 소실되었다. 한국산 건조 봉독의 복부 신전반응은 용량에 의존적으로 억제되었다. 이러한 결과에서 한국산 건조봉독은 통증치료에 있어 다른 약물을 대체하여 사용될 수 있을 것으로 생각된다.

Keywords

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