Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Identification of Differentially Expressed Genes in Four Different Growing Stages in Korea Native Chicken Liver

황갈색 재래닭의 간에서 성장 단계별 차등 발현 유전자 분석

  • Lee, K.Y. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Yu, S.L. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Jung, K.C. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Jang, B.K. (National Livestock Research Institute, R.D.A.) ;
  • Choi, K.D. (School of Biotechnology, Hankyong National University) ;
  • Lee, J.H. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • 이경연 (충남대학교 동물자원학부) ;
  • 유성란 (충남대학교 동물자원학부) ;
  • 정기철 (충남대학교 동물자원학부) ;
  • 장병귀 (농촌진흥청 축산연구소) ;
  • 최강덕 (한경대학교 생명공학부) ;
  • 이준헌 (충남대학교 동물자원학부)
  • Published : 2007.06.30
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Abstract

The chicken liver has been involved in various biological functions including detoxification, glycogen storage and plasma protein synthesis. The aim of this study was to investigate differentially expressed genes in chicken liver in four different growing stages. Using 10 arbitrary Annealing Control Primers (ACPs), five differentially expressed genes have been identified. Based on the Basic Local Alignment Search Tool (BLAST) search results, three of them were matched with previously known genes, and the other two were matched with unknown EST sequence and a hypothetical protein, respectively. In order to confirm the expression results, quantitative real-time PCR was also performed. The high similarities between the expression data using arbitrary ACPs and quantitative real-time PCR indicate that the identified genes are the real differentially expressed genes in different growing stages. The genes identified in this study can be used as valuable biomarkers in chicken with further investigation of the functions.

한국 재래닭에서 성장에 따른 유전자들의 발현 변화를 알아보고 성장 촉진, 대사 및 면역 관련 유전자를 발굴하기 위하여 주령별로 닭의 간에서 RNA를 추출하였으며 10개의 arbitrary ACPs를 이용하여 차등 발현되는 유전자를 조사하였다. 발현량에 현저한 차이를 보이는 5개의 유전자들이 선별되었으며, 이 중 3개의 유전자들은 BLAST search 결과 이미 기능이 알려진 FTH1, SAA와 HSP90B1으로 밝혀졌다. 그러나 2개의 유전자들은 닭의 genome sequence가 끝났음에도 불구하고 기능이 밝혀져 있지 않아 앞으로 이 유전자들의 기능에 대한 연구가 지속되어야 함을 의미한다. 본 연구에서 닭의 간에서 성장 단계별로 발현 차이를 보이는 유전자들은 앞으로 다른 유전자와 단백질들과의 관계를 통하여 닭의 성장 및 지방 대사를 이해하는데 도움을 줄 것으로 사료된다.

Keywords

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