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Tumorigenesis Study of Canine Adipose Derived-mesenchymal Stem Cell

개 지방세포 유래의 중간엽 줄기세포의 종양형성시험

  • Lee, Eun-Sun (Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University) ;
  • Kwon, Eun-A (Clinical Research Institute and College of Medicine, Seoul National University) ;
  • Park, Jeong-Ran (Laboratory of Stem Cell and Tumor Biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kang, Byung-Chul (Clinical Research Institute and College of Medicine, Seoul National University) ;
  • Kang, Kyung-Sun (Laboratory of Stem Cell and Tumor Biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Cho, Myung-Haing (Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University)
  • 이은선 (서울대학교 수의과대학 독성학교실) ;
  • 권은아 (서울대병원 임상의학연구소) ;
  • 박정란 (서울대학교 수의과대학 공중보건학교실) ;
  • 강병철 (서울대병원 임상의학연구소) ;
  • 강경선 (서울대학교 수의과대학 공중보건학교실) ;
  • 조명행 (서울대학교 수의과대학 독성학교실)
  • Published : 2007.09.30

Abstract

Several recent studies demonstrated the potential of bioengineering using stem cells in regenerative medicine. Adult mesenchymal stem cells (MSCs) have the pluripotency to differentiate into cells of mesodermal origin, i.e., bone, cartilage, adipose, and muscle cells; they, therefore, have many potential clinical applications. On the other hand, stem cells possess a self-renewal capability similar to cancer cells. For safety evaluation of MSCs, in this study, we tested tumorigenecity of canine adipose derived mesenchymal stem cells (cAD-MSCs) using Balb/c-nu mice. In this study, there were no changes in mortality, clinical signs, body weights and biochemical parameters of all animals treated. In addition, there were no significant changes between control and treated groups in autopsy findings. These results indicate that cAD-MSC has no tumorigenic potential under the condition in this study.

Keywords

References

  1. Arinzeh, T.L., Peter, S.J., Archambault, M.P., van den, B.C., Gordon, S., Kraus, K., Smith, A. and Kadiyala, S. (2003). Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect. J. Bone Joint Surg. Am., 85, 1927-1935
  2. Baksh, D., Song, L. and Tuan, R.S. (2004). Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J. Cell. Mol. Med., 8, 301- 316 https://doi.org/10.1111/j.1582-4934.2004.tb00320.x
  3. Barry, F.P. (2003). Mesenchymal stem cell therapy in joint disease. Novartis Found. Symp., 249, 86-96
  4. Blau, H.M., Brazelton, T.R. and Weimann, J.M. (2001). The evolving concept of a stem cell: entity or function? Cell., 105, 829-841 https://doi.org/10.1016/S0092-8674(01)00409-3
  5. Caplan, A.I. and Bruder, S.P. (2001). Mesenchymal stem cells: building blocks for molecular medicine in the 21st century. Trends Mol. Med., 7, 259-264 https://doi.org/10.1016/S1471-4914(01)02016-0
  6. Chamberlain, J.R., Schwarze, U., Wang, P.R., Hirata, R.K., Hankenson, K.D., Pace, J.M., Underwood, R.A., Song, K.M., Sussman, M., Byers, P.H. and Russell, D.W. (2004). Gene targeting in stem cells from individuals with osteogenesis imperfecta. Science, 303, 1198-1201 https://doi.org/10.1126/science.1088757
  7. Chapel, A., Bertho, J.M., Bensidhoum, M., Fouillard, L., Young, R.G., Frick, J., Demarquay, C., Cuvelier, F., Mathieu, E., Trompier, F., Dudoignon, N., Germain, C., Mazurier, C., Aigueperse, J., Borneman, J., Gorin, N.C., Gourmelon, P. and Thierry, D. (2003). Mesenchymal stem cells home to injured tissues when co-infused with hematopoietic cells to treat a radiation-induced multi-organ failure syndrome. J. Gene Med., 5, 1028-1038 https://doi.org/10.1002/jgm.452
  8. Crevensten, G., Walsh, A.J., Ananthakrishnan, D., Page, P., Wahba, G.M., Lotz, J.C. and Berven, S. (2004). Intervertebral disc cell therapy for regeneration: mesenchymal stem cell implantation in rat intervertebral discs. Ann. Biomed. Eng., 32, 430-434 https://doi.org/10.1023/B:ABME.0000017545.84833.7c
  9. Grinnemo, K.H., Mansson, A., Dellgren, G., Klingberg, D., Wardell, E., Drvota, V., Tammik, C., Holgersson, J., Ringden, O., Sylven, C. and Le Blanc, K. (2004). Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium. J. Thorac. Cardiovasc. Surg., 127, 1293-1300 https://doi.org/10.1016/j.jtcvs.2003.07.037
  10. Jackson, L., Jones, DR., Scotting, P. and Sottile, V. (2007). Adult mesenchymal stem cells: differentiation potential and therapeutic applications. J. Postgrad Med., 532, 121- 127
  11. Lee, R.H., Kim, B., Choi, I., Kim, H., Choi, H.S., Suh, K., Bae, Y.C. and Jung, J.S. (2004). Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue. Cell. Physiol. Biochem., 14, 311-324 https://doi.org/10.1159/000080341
  12. Lin, T.M., Tsai, J.L., Lin, S.D., Lai, C.S. and Chang, C.C. (2005). Accelerated growth and prolonged lifespan of adipose tissue-derived human mesenchymal stem cells in a medium using reduced calcium and antioxidants. Stem Cells Dev., 14, 92-102 https://doi.org/10.1089/scd.2005.14.92
  13. Ohnishi, S., Yanagawa, B., Tanaka, K., Miyahara, Y., Obata, H., Kataoka, M., Kodama, M., Ishibashi-Ueda, H., Kangawa, K., Kitamura, S. and Nagaya, N. (2007). Transplantation of mesenchymal stem cells attenuates myocardial injury and dysfunction in a rat model of acute myocarditis. J. Mol. Cell. Cardiol., 42, 88-97 https://doi.org/10.1016/j.yjmcc.2006.10.003
  14. Ortiz, L.A., Gambelli, F., McBride, C., Gaupp, D., Baddoo, M., Kaminski, N. and Phinney, D.G. (2003). Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc. Natl. Acad. Sci. USA, 100, 8407-8411
  15. Pansky, A., Roitzheim, B. and Tobiasch, E. (2007). Differentiation potential of adult human mesenchymal stem cells. Clin Lab., 53, 81-84
  16. Sawada, R., Ito, T. and Tsuchiya, T. (2007). Safety evaluation of tissue engineered medical devices using normal human mesenchymal stem cells. Yakugaku Zasshi, 127, 851-856 https://doi.org/10.1248/yakushi.127.851
  17. Sugaya, K. (2003). Potential use of stem cells in neuroreplacement therapies for neurodegenerative diseases. Int. Rev. Cytol., 228, 1-30 https://doi.org/10.1016/S0074-7696(03)28001-3
  18. Vilquin, J.T. and Rosset, P. (2006). Mesenchymal stem cells in bone and cartilage repair: current status. Regen Med., 1, 589-604 https://doi.org/10.2217/17460751.1.4.589