한국광학회지 (Korean Journal of Optics and Photonics)

  • 제18권5호
  • /
  • Pages.367-372
  • /
  • 2007
  • /
  • 1225-6285(pISSN)
  • /
  • 2287-321X(eISSN)
한국광학회 (Optical Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

편광 민감 광결맞음 단층 촬영 장치를 이용한 자궁경부의 조기 진단(A Preliminary Study)

Early-Stage Diagnosis of Cervix using the Polarization Sensitive Optical Coherence Tomography(A Preliminary Study)

  • 강진호 (연세대학교 보건과학대학 의공학부) ;
  • 이상원 (연세대학교 보건과학대학 의공학부) ;
  • 유지영 (연세대학교 보건과학대학 의공학부) ;
  • 강문식 (연세대학교 보건과학대학 의공학부) ;
  • 김법민 (연세대학교 보건과학대학 의공학부) ;
  • 윤보성 (연세대학교 의과대학 산부인과학교실) ;
  • 김영태 (연세대학교 의과대학 산부인과학교실) ;
  • 조남훈 (연세대학교 의과대학 병리학교실)
  • Kang, Jin-Ho (Dept. of Biomedical Engineering, Yonsei University) ;
  • Lee, Sang-Won (Dept. of Biomedical Engineering, Yonsei University) ;
  • Yoo, Ji-Yeong (Dept. of Biomedical Engineering, Yonsei University) ;
  • Kang, Moon-Sik (Dept. of Biomedical Engineering, Yonsei University) ;
  • Kim, Beop-Min (Dept. of Biomedical Engineering, Yonsei University) ;
  • Yoon, Bo-Sung (Dept. of Obstetrics Gynecology, Yonsei University College of Medicine) ;
  • Kim, Young-Tae (Dept. of Obstetrics Gynecology, Yonsei University College of Medicine) ;
  • Cho, Nam-Hoon (Dept. of Pathology, Yonsei University College of Medicine)
  • 발행 : 2007.10.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

자궁경부 상피이형성증(cervical dysplasia)은 세포핵/세포질(nuclear/cytoplasmic, N/C) 면적비의 변화를 포함하는 자궁경부 상피에서의 형태학적 변화를 일으킨다. 세포핵은 중요한 산란 원인중에 하나이기 때문에 N/C 면적비의 변화는 깊이에 따른 원형 편광장도를 반영한다. 따라서 우리는 편광 민감 광결맞음 단층촬영장치(polarization-sensitive optical coherence tomography, PS-OCT)를 이용하여 산란에 의해 발생하는 편광의 변화를 측정하였다. 자궁 경부 조직 샘플은 고등급 상피내종양(high-grade squamous intraepithelial, H-SIL)을 가진 한 명의 환자와 정상인 네 명의 환자에게서 얻어졌다. 우리는 자궁경부의 깊이에 따른 원형 편광의 유지 정도(degree of circular polarization, DOCP) 평균을 구하였고, 상피층의 깊이에서 최소 제곱 1차 선형 함수(least-square linear fit)를 사용하여 기울기를 획득하였다. 획득된 기울기를 가지고 DOCP의 변화율을 정량화 하였다. 그 결과 H-SIL에서의 DOCP의 감쇄가 정상 조직에서 보다 빠르게 나타남을 확인할 수 있었다. 이는 PS-OCT를 이용하여 산란 변화에 따른 DOCP의 변화율을 측정하는 것이 자궁경부상피 이형성증의 진단을 하는데 있어서 유용한 진단법이 될 수 있음을 의미한다.

Cervical dysplasia induces morphologic changes in the cervical epithelium which involve changes in the nuclear/cytoplasmic (N/C) ratio. Since the nucleus is one of the significant scattering sources, the N/C ratio change reflects the degree of circular polarization (DOCP) with the depth of signals. Therefore, we used the polarization-sensitive OCT (PS-OCT) technique to measure the polarization changes caused by scattering. Cervical tissues were obtained from a high-grade squamous intraepithelial lesion (H-SIL) of one woman and from low normal women. We obtained the mean of the DOCP as a function of depth in the cervix and quantified the change ratio of the DOCP using slopes that were determined by linear fits in the epithelium layer. We found that DOCP of H-SIL decayed faster than that of normal tissue because of the higher scattering in H-SIL as expected. This result indicates that the PS-OCT system might be useful in measurements of change ratio of DOCP with depth for screening of cervical dysplasia.

키워드

참고문헌

  1. A. A. Garcia and J. Bi, 'Cervical cancer,' 2004
  2. P. Athanassiadou, N. Kavantzas, M. Gonidi, P. Athanassiades, A. Liossi, L. Nakopoulou, E. Petrakakou, and P. Davaris, 'Correlations between Nuclear/Cytoplasmic Area Ratio and Classification of Cervical Smears,' J. Exp. Clin. Cancer Res., vol. 20, no .4, pp. 481-486, 2001
  3. R. Hornung, T. H. Pham, K. A. Keefe, M. W. Berns, Y. Tadir, and B. J. Tromberg, 'Quantitiative near-intrared spectroscopy of cervical dysplasia in vivo,' Human Reproduction, vol. 14, no. 11, pp. 2908-2916, 1999 https://doi.org/10.1093/humrep/14.11.2908
  4. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, 'Optical coherence tomography,' Science, vol. 254, pp. 1178-1181, 1991 https://doi.org/10.1126/science.1957169
  5. F. I. Feldchtein, G. V. Gelikonov, V. M. Gelikonv, R. V. Kuranov, and A. M. Sergeev, 'Endoscopic applications of optical coherence tomography,' Opt. Express, vol. 3, no. 6, pp. 257-270, 1998 https://doi.org/10.1364/OE.3.000257
  6. A. Zuluaga, M. Follen, I. Boiko, A. Malpica, and R. Richards-Kortum, 'Optical coherence tomography: A pilot study of a new imaging technique for noninvasive examination of cervical tissue,' Am. J. Obstet. and Gynoco., vol. 193, pp. 83-88, 2005 https://doi.org/10.1016/j.ajog.2004.11.054
  7. J. F. de Boer, S. M. Srinivas, A. Malekafzali, Z. Chen, and J. S. Nelson, 'Imaging thermally damaged tissue by polarization sensitive optical coherence tomography,' Opt. Express, vol. 3, no. 6, pp. 212-218 https://doi.org/10.1364/OE.3.000212
  8. M. G. Ducros, J. F. de Boer, H. Huang, L. C. Chao, and Z. Chen, 'Polarization Sensitive Optical Coherence Tomography of the Rabbit Eye,' IEEE J. Select. Topics Quantum Electron., vol. 5, no. 4, pp. 1159-1167, 1999 https://doi.org/10.1109/2944.796342
  9. J. F. de Boer and T. E. Milner, 'Review of polarization sensitive optical coherence tomography and Stokes vector determination,' J. Biomed. Opt., vol. 7, no. 3, pp. 359-371, 2002 https://doi.org/10.1117/1.1483879
  10. S.-W. Lee, J.-T. Oh, and B.-M. Kim, 'Microscopic Imaging of Articular Cartilage using Polarization-Sensitive Optical Coherecen Tomography,' J. Biomed. Eng. Res., vol. 26, no.1, pp. 33-38, 2005
  11. A. D. Kim and M. Moscoso, 'Influence of the relative refractive index on the depolarization of multiply scattered,' Phys. Rev. E., vol. 64, no. 2, 026612, 2001 https://doi.org/10.1103/PhysRevE.64.026612
  12. J. Li, G. Yao, and L. V. Wang, 'Degree of polarization in laser speckles from turbid media: Implications in tissue optics,' J. Biomed. Opt., vol. 7, no. 3, pp. 307-312, 2002 https://doi.org/10.1117/1.1483313
  13. X. Wang, L. V. Wang, C. Sun, and C. Yang, 'Polarized light propagation through scattering media: time-resolved Monte Carlo simulations and experiments,' J. Biomed. Opt., vol. 8, no. 4, pp. 608-617, 2003 https://doi.org/10.1117/1.1606462
  14. A. M. Rollins, M. D. Kulkarni, S. Yazdanfar, R. Ungarunyawee, and J. A. Izatt, 'In vivo video optical coherence tomography,' Opt. Express, vol. 3, no. 6, pp. 219-229, 1998 https://doi.org/10.1364/OE.3.000219
  15. W. K. Niblack, J. O. Schenk, and M. E. Brezinski, 'Dispersion in a grating-based optical delay line for optical coherence tomography,' Appl. Opt., vol. 42, no. 19, pp. 4115-4118, 2003 https://doi.org/10.1364/AO.42.004115
  16. M. Kinnunen, R. Myllyla, T. Jokela, and S. Vainio, 'In vitro studies toward noninvasive glucose monitoring with optical coherence tomography,' Appl. Opt., vol. 45, no. 10, pp. 2251-2260, 2006 https://doi.org/10.1364/AO.45.002251