International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Molecular Characterization of A Glycine and Proline-rich Antibacterial Protein from Larvae of A Beetle, Protaetia brevitarsis

  • Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Seong-Ryul (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kang, Heui-Yun (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Yun, Eun-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Ahn, Mi-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Park, Kwan-Ho (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Jeon, Jae-Pil (Korea National Institute of Health) ;
  • Kim, Mi-Ae (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Nam-Jung (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Hwang, Seok-Jo (Korea National Institute of Health) ;
  • Kim, Ik-Soo (College of Agriculture & Life science, Chonnam National University)
  • Published : 2007.09.30
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Abstract

A glycine and proline-rich antibacterial protein was cloned from larvae of a beetle, Protaetia brevitarsis. The DNAs encoded a deduced propeptide of 127 amino acid residues with predicted molecular weight of 14.0 kDa and PI of 7.89. Structural analysis of this protein indicated the presence of a recognition sequence for the cleavage site within the constitutive secretory pathway(Arg-Xaa-Lys/Arg-Arg), suggesting that mature portion(72 amino acid residues) is produced by cleavage of signal peptide and propeptide from 127 amino-acid-long precursor protein. Mature portion sequence of this protein showed 72% similarity to that of Oryctes rhinoceros Rhinocerosin and 91% to that of Holotrichia diomphalia holotricin 2. The mRNA expression was reached the highest level at 4 hrs after E. coli injection and then declined gradually.

Keywords

References

  1. Baba, K., M. Okada, H. Kawano and S. Natori (1987) Purification of sarcophagi peregrine. J. Biochem. 102, 69-74 https://doi.org/10.1093/oxfordjournals.jbchem.a122042
  2. Bullet, P., S. Cociancich, J.-L. Dimarcq, J. Lambert, J.-M. Rechhart, D. Hoffmann, C. Hetru and J. A. Hoffmann (1991) Insect immunity: Isolation from a coleopteran insect a novel inducible antibacterial peptide and of new members of the insect defencin family. J. Biol. Chem. 266, 24520-24525
  3. Dimarcq, J. K., D. Zachary, J. A. Hoffmann, D. Hoffmann and J. M. Reichhart (1990) Insect immunity: expression of the two major inducible antibacterial peptides, defensin and diptericin, in Phormia terranovae. EMBO J. 9, 2507-2515
  4. Hosaka, M., M. Nagahama, W. S. Kim, T. Watanabe, K. Hatsuzawa, J. Ikemizu, K. Murakami and K. Nakayama (1992) Arg-X-Lys/Arg-Arg motif as a signal for precursor cleavage catalyzed by furin within the constitutive secretory pathway. J. Biol. Chem. 266,12127-12130
  5. Hultmark, D. (1993) Immune reactions in Drosophila and other insects: a model for innate immunity. Trends Genet. 9, 178-183 https://doi.org/10.1016/0168-9525(93)90165-E
  6. Kim, C.-H., J.-S. Lee, M.-S. Go and K.- T. Park (2002) Ecological characteristics of Protaetia orientalis submarmorea (Burmeister) (coleopteran: Centoniidae) Korean J. Entomol. 41,43-47
  7. Lee, S. Y., H. J. Moon, S. Kurata, T. Kurama, S. Natori and B. L. Lee (1994) Purification and molecular cloning of cDNA for an inducible antibacterial protein of larvae of a coleopteran Insect, Holotrichia diomphalia. J. Biochem. 115, 82-86 https://doi.org/10.1093/oxfordjournals.jbchem.a124309
  8. Sagisaka, A., A. Miyanoshita, J. Ishibashi and M. Yamakawa (2001) purification, characterization and gene expression of a glycine and proline-rich antibacterial protein family from larvae of a beetle, Alloomyrina dichotoma. Insect Mol. Biol. 10, 293-302 https://doi.org/10.1046/j.0962-1075.2001.00261.x
  9. Thomson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin and D. G. Higgins (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24, 173-216
  10. Wicker, C., J. M. Reichhart, D. Hoffmann, C. Samakovlis and J. A. Hoffmann (1990) Insect immunity: characterization of a Drosophila cDNA encoding a novel member ofthe diptericin family of immune peptides. J Biol. Cherm. 265, 224932-2498
  11. Yang, J., Yamamoto, M., Ishibashi, J., Taniai, K. and Yamakawa, M.(1998) Isolation, cDNA cloning and gene expression of an antibacterial protein from larvae of the coconut rhinoceros beetle, Oryctes rhinoceros. Eur. J. Biochem. 255 : 734-738 https://doi.org/10.1046/j.1432-1327.1998.2550734.x