Isolation and Structure Determination of Streptochlorin, an Antiproliferative Agent from a Marine-derived Streptomyces sp. 04DH110

  • Shin, Hee-Jae (Marine Natural Products Laboratory, Korea Ocean Research & Development Institute) ;
  • Jeong, Hyun-Sun (Marine Natural Products Laboratory, Korea Ocean Research & Development Institute) ;
  • Lee, Hyi-Seung (Marine Natural Products Laboratory, Korea Ocean Research & Development Institute) ;
  • Park, Song-Kyu (Bio-evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hwan-Mook (Bio-evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Ho-Jeong (Chemical Genomics Laboratory, Department of Biotechnology, College of Engineering, Yonsei University)
  • Published : 2007.08.30

Abstract

An antiproliferative agent, streptochlorin, was isolated from the fermentation broth of a marine actinomycete isolated from marine sediment. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Streptomyces. Bioactivity guided fractionation of the culture extract by solvent partitioning, ODS open flash chromatography, and reversed-phase HPLC gave a pure compound, streptochlorin. Its structure was elucidated by extensive 2D NMR and mass spectral analyses. Streptochlorin exhibited significant antiproliferative activity against human cultured cell lines.

Keywords

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