Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Comparison of Diglyceride, Conjugated Linoleic Acid, and Diglyceride-Conjugated Linoleic Acid on Proliferation and Differentiation of 3T3-L1 Preadipocytes

  • Jeong, Jae-Hwang (College of Liberal Arts, Seowon University) ;
  • Lee, Sang-Hwa (Department of Food and Nutrition, Seowon University) ;
  • Hue, Jin-Joo (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Yea-Eun (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Young-Ho (Ilshinwells Co., Ltd) ;
  • Hong, Soon-Ki (Ilshinwells Co., Ltd) ;
  • Jeong, Seong-Woon (Ilshinwells Co., Ltd) ;
  • Nam, Sang-Yoon (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Yun, Young-Won (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Beom-Jun (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Published : 2007.09.30
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Abstract

Conjugated linoleic acid (CLA) reduces fat deposition in several mammalian species. The proposed mechanisms for this effect are reduced preadipocyte proliferation and differentiation. The objective of this study was to investigate the inhibitory effects of diglyceride (DG), CLA, DG-CLA of proliferation and differentiation of 3T3-L1 preadipocytes. Cell viability was determined using WST-8 analysis and cell differentiation was determined by glycerol-3-phosphate dehydrogenase (GPDH) activity. Lipid accumulation in differentiating 3T3-L1 cells was measured by Oil red O staining. The proliferation of preconfluent 3T3-L1 cells by treatments of DG, CLA, and DG-CLA was reduced in a dose-dependent manner. CLA among them was the most effective in reduction of viable cells with increasing concentrations. Treatments of the DG, CLA, and DG-CLA at the concentration of $100{\cdot}\ddot{I}g/ml$ for 48h significantly inhibited differentiation of 3T3-L1 cells (p<0.05). In addition. cytoplasmic lipid accumulation during differentiation of the 3T3-L1 preadipocytes was also inhibited by treatments of the test solutions. DG-CLA was the most effective in the inhibition of differentiation and lipid accumulation in 3T3-L1 cells. These results indicate that the DG including CLA as fatty acids is more effective for anti-obesity than DG or CLA alone and that consumption of DG-CLA as a dietary oil may give a benefit for controlling overweight in humans.

Conjugated linoleic acid (CLA)는 전지방세포의 증식 및 분화를 억제시키므로서 포유동물에서 지방의 축적을 감소시키는 것으로 알려져 있다. 논 연구의 목적은 diglyceride(DG), CLA, 및 DG-CLA가 전지방세포의 증식 및 분화를 억제시키는지를 알아보고자, 세포생존측정, glycerol-3-phosphate dehydrogenase (GPDH) 활성측정, 지방축적에 대한 Oil red O 염색을 실시하였다. DG, CLA, 및 DG-CLA의 처치는 전지방세포의 증식을 농도 의존적으로 감소시켰으며, 그러한 효과는 CLA에서 가장 강하게 나타났다. 이들 시험물질의 $100 {\mu}g/ml$ 농도에서 DG, CLA, DG-CLA는 유의적으로 GPDH의 활성을 낮추었으며(p<0.05), 이러한 결과는 전지방세포의 분화를 억제하는 것을 의미한다. 더불어 이들 시험물질은 전지방세포의 분화과정 중에 지방축적을 효과적으로 억제시켰다. 더욱이 DG-CLA가 DG 혹은 CLA보다 분화 및 지방축적에 대해 더 강한 효과를 보였다. 이러한 결과로부터 DG-CLA의 섭취는 사람에서 체중조절의 유용한 효과를 얻을 수 있을 것이다.

Keywords

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