Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Effect of Oral Immunization with Liposome-Entrapped Bacterial Antigen on Protection Against Experimental Aeromonas Hydrophila

  • Choi, Sang-Hoon (Department of Aquatic Medicine, Kunsan National University) ;
  • Oh, Chan-Ho (Department of Food Biotechnology, Woosuk University)
  • Published : 2007.06.30
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Abstract

Liposome-entrapped atypical Aeromonas hydrophila antigen was prepared to investigate the potential protective efficacy for A. hydrophila infection. Carp (Cyprinus carpio) were immunized orally with liposome-entrapped A. hydrophila antigen. After immunization, significantly more antigen-specific antibodies were detected in serum, intestinal mucus and bile than non-immunized control group. The immunized carp were then challenged by immersion with $1{\times}10^{6}$ cfu/ml of A. hyrdophila for 60 min. Of the eight non-immunized carp, three carp died (62.5% survival), whereas five out of six (83.5%) of the immunized survived. Furthermore, development of skin ulcers was significantly inhibited in carp immunized with liposomes containing A. hydrophila antigen. These results suggest that liposomes containing A. hydrophila antigen have a potential for induction of protective immune responses against atypical A. hydrophila infection and also suggest the possibility of developing a vaccine that may ultimately be used for prevention of fish diseases.

Keywords

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