Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effects of Culture Conditions on Osteogenic Differentiation in Human Mesenchymal Stem Cells

  • Song, Su-Jin (Department of Chemical Engineering, Hanyang University) ;
  • Jeon, O-Ju (Department of Chemical Engineering, Hanyang University) ;
  • Yang, Hee-Seok (Department of Bioengineering, Hanyang University) ;
  • Han, Dong-Keun (Biomaterials Research Center, Korea Institute of Science and Technology) ;
  • Kim, Byung-Soo (Department of Bioengineering, Hanyang University)
  • Published : 2007.07.31
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Abstract

Human bone marrow-derived mesenchymal stem cells (hBMMSCs) must differentiate into osteogenic cells to allow for successful bone regeneration. In this study, we investigated the effects of different combinations of three soluble osteogenic differentiation-inducing factors [L-ascorbic acid (AC), ${\beta}$-glycerophosphate (${\beta}G$), and bone morphogenic protein-2 (BMP-2)] and the presence of a hydroxyapatite (HA) substrate on hBMMSC osteogenic differentiation in vitro. hBMMSCs were cultured in medium containing various combinations of the soluble factors on culture plates with or without HA coating. After 7 days of culture, alkaline phosphatase (ALP) activity, calcium deposition, and osteoprotegerin (OPG) and osteopontin (OPN) expression were measured. The effects of individual and combined factors were evaluated using a factorial analysis method. BMP-2 predominantly affected expression of early markers of osteogenic differentiation (ALP and OPG). HA had the highest positive effect on OPN expression and calcium deposition. The interaction between AC, ${\beta}G$, and HA had the second highest positive effect on ALP activity.

Keywords

References

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