Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Ethanol Extract of Fermented Soybean, Chungkookjang, Inhibits the Apoptosis of Mouse Spleen, and Thymus Cells

  • Kim, Han-Bok (Department of Biotechnology, Hoseo University) ;
  • Lee, Hye-Sung (School of Biotechnology & Biomedical Science and Biohealth Product Research Center, Inje University) ;
  • Kim, Sook-Jin (School of Biotechnology & Biomedical Science and Biohealth Product Research Center, Inje University) ;
  • Yoo, Hyung-Jae (Department of Biotechnology, Hoseo University) ;
  • Hwang, Jae-Sung (Department of Biotechnology, Hoseo University) ;
  • Chen, Gang (Department of Chemistry, Fudan University) ;
  • Youn, Hyun-Joo (School of Biotechnology & Biomedical Science and Biohealth Product Research Center, Inje University)
  • Published : 2007.06.30
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Abstract

Apoptosis is a step of the cell cycle which is important in the regulation of immune cell populations. Chungkookjang is a Korean traditional fermented soybean containing microorganisms, enzymes, and bioactive compounds which was used in the treatment of mouse spleen as well as thymus cells (CH1-fermented soybean containing barley, wormwood, and sea tangle; CH2-fermented soybean) and was found to exhibit substantially reduced small DNA fragmentation. An MTT assay showed that the treatment of CH1 and CH2 into the mouse splenocytes and thymocytes sharply increased their survival. Moreover, a FACS analysis also showed that CH1 and CH2 are effective at suppressing the apoptosis of splenocytes and thymocytes. The fermented soybean isoflavone concentrations, which are implicated in lowering breast and prostate cancers, lowering the risk of cardiovascular diseases, and improving bone health, were determined using Capillary Electrophoresis-Electrochemical Detection (CE-ED). The amount of Daidzein in fermented soybean significantly increased by 44-fold dramatically, compared with those in unfermented soybean. In this study, we demonstrated that ethanol extracts of Chungkookjang promote the survival of the mouse spleen and thymus cells in culture by suppressing their apoptotic death. Future studies should investigate which genes are related to apoptosis of the immune cells.

Keywords

References

  1. Aggarwal, B.B. 2003. Signaling pathways of the TNF superfamily: A double-edged sword. Nat. Rev. Immunol. 3, 745-756 https://doi.org/10.1038/nri1184
  2. Aggarwal, B.B. and S. Shishodia. 2006. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem. Pharmacol. 71, 1397-1421 https://doi.org/10.1016/j.bcp.2006.02.009
  3. Eray, M., M. Matto, M. Kaartinen, L.C. Andersson, and J. Pelkonen. 2001. Flow cytometric analysis of apoptotic subpopulations with a combination of Annexin V-FITC, promium iodide, and SYTO 17. Cytometry 43, 134-142 https://doi.org/10.1002/1097-0320(20010201)43:2<134::AID-CYTO1028>3.0.CO;2-L
  4. Hsieh, C.Y., R.C. Santell, S.Z. Haslam, and W.G. Helferich. 1998. Estrogen effects of genistein on the growth of estrogen receptorpositive human breast cancer (MCF-7) cells in vitro and in vivo. Cancer Res. 58, 3833-3838
  5. Hwang, J.S., H.K. Chung, E.K. Bae, A.Y. Lee, H.J. Ji, D.W. Park, H.J. Jung, C.W. Cho, H.J. Choi, D.S. Lee, K.R. Lee, and H.J. Youn. 2003. The polysaccharide fraction AIP1 from Artemisia iwayomogi suppresses apoptotic death of the mouse spleen cells in culture. Arch. Pharm. Res. 26, 294-300 https://doi.org/10.1007/BF02976958
  6. Hwang, J.S., H.J. Ji, K.A. Koo, N.H. Lee, H.K. Yeo, S.W. Cheong, J.H. Park, G.S. Oh, C.S. Yoon, and H.Y. Youn. 2005. AIP1, a water-soluble fraction from Artemisia iwayomogi, suppresses thymocytes apoptosis in vitro and down-regulates the expression of Fas gene. Biol. Pharm. Bull. 28, 921-924 https://doi.org/10.1248/bpb.28.921
  7. Ji, H.J., H.K. Yeo, N.H. Lee, J.S. Hwang, K.A. Koo, S.W. Cheong, J.H. Park, G.S. Oh, C.S. Yoon, and H.Y. Youn. 2005. A carbohydrate fraction, AIP1, from Artemisia iwayomogi down-regulates Fas gene expression and suppresses apoptotic death of the thymocytes induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Biotechnol. Lett. 27, 253-257 https://doi.org/10.1007/s10529-004-8294-2
  8. Kissil, J.L., O. Cohen, T. Raveh, and A. Kimchi. 1999. Structurefunction analysis of an evolutionary conserved protein DAP3, which mediates TNF-alpha- and Fas-induced cell death. EMBO J. 18, 353-362 https://doi.org/10.1093/emboj/18.2.353
  9. Kim, K.H., Y.W. Kim, H.B. Kim, B.J. Lee, and D.S. Lee. 2006. Antiapoptotic activity of laminarin polysaccharides and their enzymatically hydrolyzed oligosaccharides from Laminaria japonica. Biotechnol. Lett. 28, 439-446 https://doi.org/10.1007/s10529-005-6177-9
  10. Lee, J.J., D.S. Lee, and H.B. Kim. 1999. Fermentation patterns of Chungkookjang and Kanjang by Bacillus licheniformis B1. Kor. J. Microbiol. 35, 296-301
  11. Nagata, S. and P. Golstein. 1995. The Fas death factor. Science 267, 1449-1456 https://doi.org/10.1126/science.7533326
  12. Newmeyer, D.D. and S. Ferguson-Miller. 2003. Mitochondria: Releasing power for life and unleashing the machineries for death. Cell 112, 481-490 https://doi.org/10.1016/S0092-8674(03)00116-8
  13. Peng, Y.Y. and J.N. Ye. 2006. Determination of isoflavones in red clover by capillary electrophoresis with electrochemical detection. Fitoterapia 77, 171-178 https://doi.org/10.1016/j.fitote.2006.01.004
  14. Tang, D., J.H. Wang, Y. Fan, Y.L. Yang, Z.G. Tian, J. Luo, G.P. Yang, and F. Zhang. 2006. Cloning of ${\beta}$-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC0635) and its expression in Escherichia coli BL21(DE3). Appl. Microbiol. Biotechnol. 72, 705-712
  15. Yoo, H.J., D.S. Lee, and H.B. Kim. 2004. Chungkookjang fermentation of mixture of barley, wormwood, sea tangle, and soybean. Kor. J. Microbiol. 40, 49-53