Expression of Escherichia coli DcuS-R Two-Component Regulatory System is Regulated by the Secondary Internal Promoter Which is Activated by CRP-cAMP

  • Oyamada, Tomoya (Department of Applied Chemistry, National Defense Academy of Japan) ;
  • Yokoyama, Katsushi (National Institute of Advanced Industrial Science and Technology (AIST)) ;
  • Morinaga, Michiko (Department of Applied Chemistry, National Defense Academy of Japan) ;
  • Suzuki, Masashi (National Institute of Advanced Industrial Science and Technology (AIST)) ;
  • Makino, Kozo (Department of Applied Chemistry, National Defense Academy of Japan)
  • 발행 : 2007.06.30

초록

The DcuS-R two-component system of Escherichia coli senses $C_{4}-dicarboxylates$ of the medium and regulates expression of the genes related to utilization of them. It is known that phospho-DcuR induces expression of genes such as the dcuB-fumB operon, the frdABCD operon, and the dctA gene. We analyzed promoters of the dcuS-R operon to elucidate the transcriptional regulation system. We found a novel internal promoter within the dcuS gene that is regulated by the transcriptional regulator, CRP-cAMP, in both aerobic and anaerobic conditions.

키워드

참고문헌

  1. Abo-Amer, A.E., J. Munn, K. Jackson, M. Aktas, P. Golby, D.J. Kelly, and S.C. Andrews. 2004. DNA interaction and phosphotransfer of the C4-dicarboxylate-responsive DcuS-DcuR twocomponent regulatory system from Escherichia coli. J. Bacteriol. 186, 1879-1889 https://doi.org/10.1128/JB.186.6.1879-1889.2004
  2. Cecchini, G., I. Schröder, R.P. Gunsalus, and E. Maklashina. 2002. Succinate dehydrogenase and fumarate reductase from Escherichia coli. Biochim. Biophys. Acta. 1553, 140-157 https://doi.org/10.1016/S0005-2728(01)00238-9
  3. Davies, S.J., P. Golby, D. Omrani, S.A. Broad, V.L. Harrington, J.R. Guest, D.J. Kelly, and S.C. Andrews. 1999. Inactivation and regulation of the aerobic C4-dicarboxylate transport (dctA) gene of Escherichia coli. J. Bacteriol. 181, 5624-5635
  4. Engel, P., R. Kramer, and G. Unden. 1992. Anaerobic fumarate transport in Escherichia coli by an fnr-dependent dicarboxylate uptake system which is different from aerobic dicarboxylate uptake. J. Bacteriol. 174, 5533-5539 https://doi.org/10.1128/jb.174.17.5533-5539.1992
  5. Goh, E.B., P.J. Bledsoe, L.L. Chen, P. Gyaneshwar, V. Stewart, and M.M. Igo. 2005. Hierarchical control of anaerobic gene expression in Escherichia coli K-12: the nitrate-responsive NarXNarL regulatory system represses synthesis of the fumarate-responsive DcuS-DcuR regulatory system. J. Bacteriol. 187, 4890-4899 https://doi.org/10.1128/JB.187.14.4890-4899.2005
  6. Golby, P., D.J. Kelly, J.R. Guest, and S.C. Andrews. 1998. Transcriptional regulation and organization of the dcuA and dcuB genes, encoding homologous anaerobic $C_{4}$-dicarboxylate transporters in Escherichia coli. J. Bacteriol. 180, 6586-6596
  7. Golby, P., S. Davies, D.J. Kelly, J.R. Guest, and S.C. Andrews. 1999. Identification and characterization of a two-component sensorkinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to $C_{4}$-dicarboxylates in Escherichia coli. J. Bacteriol. 181, 1238-1248
  8. Green, J., C. Scott, and J.R. Guest. 2001. Functional versatility in the CRP-FNR superfamily of transcription factors: FNR and FLP. Adv. Microbial. Physiol. 44, 1-34 https://doi.org/10.1016/S0065-2911(01)44010-0
  9. Guest, J.R. 1992. Oxygen-regulated gene expression in Escherichia coli. J. Gen. Microbiol. 138, 2253-2263 https://doi.org/10.1099/00221287-138-11-2253
  10. Ishihama, A. 2000. Functional modulation of Escherichia coli RNA polymerase. Annu. Rev. Microbiol. 54, 499-518 https://doi.org/10.1146/annurev.micro.54.1.499
  11. Iuchi, S. and E.C.C. Lin. 1995. Signal transduction in the Arc system for control of operons encoding aerobic respiratory enzymes, p. 223-231. In Hoch, J.A., Silhavy, T.J. (eds.). Two-component signal transduction. ASM Press, Washington, D.C., USA
  12. Janausch, I.G., E. Zientz, Q.H. Tran, A. Kroger, and G. Unden. 2002a. $C_{4}$-dicarboxylate carriers and sensors in bacteria. Biochim. Biophys. Acta. 1553, 39-56 https://doi.org/10.1016/S0005-2728(01)00233-X
  13. Janausch, I.G., I. Garcia-Moreno, and G. Unden. 2002b. Function of DcuS from Escherichia coli as a fumarate-stimulated histidine protein kinase in vitro. J. Biol. Chem. 277, 39809-39814 https://doi.org/10.1074/jbc.M204482200
  14. Kay, W.W. and H.L. Kornberg. 1971. The uptake of $C_{4}$ dicarboxylic acids by Escherichia coli. Eur. J. Biochem. 18, 274-281 https://doi.org/10.1111/j.1432-1033.1971.tb01240.x
  15. Lancaster, C.R.D. 2002. Succinate: quinine oxdoreductases: an overview. Biochim. Biophys. Acta. 1553, 1-6 https://doi.org/10.1016/S0005-2728(01)00240-7
  16. Miller, J.H. 1992. A short course in bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA
  17. Six, S., S.C. Andrews, G. Unden, and J.R. Guest. 1994. Escherichia coli possesses two homologous anaerobic $C_{4}$-dicarboxylate memrane transporters (DcuA and DcuB) distinct from the aerobic dicarboxylate transport system (Dct). J. Bacteriol. 176, 6470-6478 https://doi.org/10.1128/jb.176.21.6470-6478.1994
  18. Spiro, S., K. Gaston, A. Bell, R. Roberts, S. Busby, and J. Guest. 1990. Interconversion of the DNA-binding specificities of the two related transcription regulators, CRP and FNR. Mol. Microbiol. 4, 1831-1838 https://doi.org/10.1111/j.1365-2958.1990.tb02031.x
  19. Tran, Q.H. and G. Unden. 1998. Changes in the proton potential and the cellular energetics of Escherichia coli during growth by aerobic and anaerobic respiration or by fermentation. Eur. J. Biochem. 251, 538-543 https://doi.org/10.1046/j.1432-1327.1998.2510538.x
  20. Tran, Q.H., J. Bongaerts, D. Vlad, and G. Unden. 1997. Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications. Eur. J. Biochem. 244, 155-160 https://doi.org/10.1111/j.1432-1033.1997.00155.x
  21. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33, 103-119 https://doi.org/10.1016/0378-1119(85)90120-9
  22. Yokoyama, K., T. Oyamada, M. Suzuki, and K. Makino. 2005. Construction of cloning system for CRP-cAMP dependent promoters in Escherichia coli. Mem. Natl. Def. Acad. Jpn. 45, 1-9
  23. Zientz, E., J. Bongaerts, and G. Unden. 1998. Fumarate regulation of gene expression in Escherichia coli by DcuSR (dcuSR genes) two-component regulatory system. J. Bacteriol. 180, 5421-5425
  24. Zientz, E., S. Six, and G. Unden. 1996. Identification of a third secondary carrier (DcuC) for anaerobic $C_{4}$-dicarboxylate transport in Escherichia coli: roles of the three Dcu carriers in uptake and exchange. J. Bacteriol. 178, 7241-7247 https://doi.org/10.1128/jb.178.24.7241-7247.1996