Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Expression of Escherichia coli DcuS-R Two-Component Regulatory System is Regulated by the Secondary Internal Promoter Which is Activated by CRP-cAMP

  • Oyamada, Tomoya (Department of Applied Chemistry, National Defense Academy of Japan) ;
  • Yokoyama, Katsushi (National Institute of Advanced Industrial Science and Technology (AIST)) ;
  • Morinaga, Michiko (Department of Applied Chemistry, National Defense Academy of Japan) ;
  • Suzuki, Masashi (National Institute of Advanced Industrial Science and Technology (AIST)) ;
  • Makino, Kozo (Department of Applied Chemistry, National Defense Academy of Japan)
  • Published : 2007.06.30
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Abstract

The DcuS-R two-component system of Escherichia coli senses $C_{4}-dicarboxylates$ of the medium and regulates expression of the genes related to utilization of them. It is known that phospho-DcuR induces expression of genes such as the dcuB-fumB operon, the frdABCD operon, and the dctA gene. We analyzed promoters of the dcuS-R operon to elucidate the transcriptional regulation system. We found a novel internal promoter within the dcuS gene that is regulated by the transcriptional regulator, CRP-cAMP, in both aerobic and anaerobic conditions.

Keywords

References

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