Journal of Dairy Science and Biotechnology
- Volume 25 Issue 2
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- Pages.17-20
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- 2007
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- 2733-4554(pISSN)
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- 2733-4562(eISSN)
Production of Milk-Originated Antimicrobial Peptide, Lactoferricin, in E. coli
미생물을 이용한 우유 유래 항균펩타이드(락토페리신)의 생산
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Kang, Dae-Kyung
(Department of Animal Resources Science, School of Bio-Resources Science, Dankook University)
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강대경
(단국대학교 동물자원학과)
- Published : 2007.11.30
Abstract
Bovine lactoferricin(LFcin B) is a peptide of 25 amino acids that originated from the N terminus of bovine lactoferrin, and is characterized as having potent antimicrobial activity against bacteria, fungi, protozoa and viruses. But, direct expression of Lfcin B is lethal to Escherichia coli. For the efficient production of Lfcin B in microorganism, we developed an expression system in which the gene for cationic Lfcin B was fused to an anionic peptide gene, and successfully expressed the concatemeric fusion gene in E. coli. The purified recombinant Lfcin B was found to have antimicrobial activity, as chemically synthesized Lfcin B peptide does.