한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제24권2호
  • /
  • Pages.197-200
  • /
  • 2007
  • /
  • 1598-298X(pISSN)
  • /
  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

생후 5개월된 형질전환 암송아지에서의 인공 비유

Artificial Induction of Lactation in 5 Month Old Transgenic Female Calf

  • 김영훈 (충남대학교 수의과대학) ;
  • 이창우 (서울대학교 수의과대학) ;
  • 한용만 (한국과학기술원 생명과학과) ;
  • 이경광 (한국생명공학연구원 동물발생공학연구실) ;
  • 조종기 (충남대학교 수의과대학) ;
  • 신상태 (충남대학교 수의과대학)
  • Kim, Young-Hun (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Chang-Woo (College of Veterinary Medicine, Seoul National University) ;
  • Han, Yong-Mahn (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Lee, Kyung-Kwang (Center of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Jong-Ki (College of Veterinary Medicine, Chungnam National University) ;
  • Shin, Sang-Tae (College of Veterinary Medicine, Chungnam National University)
  • 발행 : 2007.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The purpose of this study was premature induction of lactation in the transgenic calf to confirm the expression of human lactoferrin gene in the milk as early as possible. We performed the induction of lactation in 6 normal 1-month old calves and 3 normal and 1 transgenic 5-month old calves. In order to induction of lactation, 1-month old calf was injected with estradiol benzoate (0.2 mg/kg), $17-{\beta}$ estradiol cypionate (0.1 mg/kg) and progesterone (0.25-0.5 mg/kg) in every other day by 21 days. After 10 days cessation of administration, dexamethasone (0.028 mg/kg) was injected intramuscularly, however, lactation was failed. In the 5-month old calves, estradiol benzoate (0.2 mg/kg) and progesterone (0.5 mg/kg) were injected individually every other day during 21 days. After 10 days cessation of administration, dexamethasone (0.1 mg/kg) was injected intramuscularly and milk was lactated in the next day. Lactation was successfully induced in the 5-month old normal and transgenic calves and we confirmed the lactoferrin gene in the milk of transgenic calf. In conclusion, confirmation of human lactoferrin gene expressed in the milk of the transgenic calf was possible in 20 months earlier than in normal condition.

키워드

참고문헌

  1. Akers RM, Bauman DE, Capuco AV, Goodman GT, Tucker HA. Prolactin regulation of milk secretion and biochemical differentiation of mammary epithelial cells in periparturient cows. Endocrinology 1981; 109: 23-3 1 https://doi.org/10.1210/endo-109-1-23
  2. Collier RJ, Croom WJ, Bauman DE, Hays RL, Nelson DR. Cellular studies of mammary tissue from cows hormonally induced into lactation: lactose and fatty acid synthesis. J Dairy Sci 1976; 59: 1226-123 1 https://doi.org/10.3168/jds.S0022-0302(76)84351-2
  3. Croom WJ, Collier RJ, Bauman DE, Hays RL, Nelson DR Cellular studies of mammary tissue from cows hormonally induced into lactation: histology and ultrastructure. J Dairy Sci 1976; 59: 1232-1246 https://doi.org/10.3168/jds.S0022-0302(76)84352-4
  4. Dabas YP, Sud SC. Induction of lactation in cattle with estradiol 17-beta and progesterone primed progesterone. Indian J Exp Bioi 1989; 27: 774-776
  5. Delouis G, Djiane J, houdebine LM, Terqui M. Relation between hormones and mammary gland function J Dairy Sci 1980; 63: 1492 https://doi.org/10.3168/jds.S0022-0302(80)83110-9
  6. Erb RE. Hormonal control of mammogenesis and onset of lactation in cows-a review. J Dairy Sci 1977; 60: 155 https://doi.org/10.3168/jds.S0022-0302(77)83849-6
  7. Fleming JR, Head HR, Bachman KC, Becker HN, Wilcox CJ. Induction of lactation: histological and biochemical development of mammary tissue and milk yields of cows injected with estradiol-17 beta and progesterone for 21 days. J Dairy Sci 1986; 69: 3008-3021 https://doi.org/10.3168/jds.S0022-0302(86)80764-0
  8. Fulkerson WJ. Artificial induction of lactation in maiden heifers. Theriogenology 1997; 8: 136 https://doi.org/10.1016/0093-691X(77)90098-X
  9. Gupta N. Induction of lactation in a cross bred cow-a case report. The Indian veterinary journal 2003; 80: 447-448
  10. Hooda OK, Kaker ML, Dhanda OP, Galhotra MM, Razdan M N. Induction of lactation and retroductive response in nonproducing buffalo heifers folloing steroid hormone treatment. Asian Austrian journal of animal science 1997; 10: 519-522 https://doi.org/10.5713/ajas.1997.519
  11. Kim SJ, Lee KW, Yu DY, Han YM, Lee CS, Nam MS, Moon HB, Lee KK. Expression analysis of a bovine a-caseinJhuman lactoferrin hybrid gene in transgenic mice. J Reproduction and Development 1997; 43: 143-149 https://doi.org/10.1262/jrd.43.143
  12. Lee CS, Kim KJ, Yu DY, Lee KK. An efficient production of human growth hormone in the milk of transgenic mice using rat ${\beta}$-casein/hGH fusion genes. Applied Biochemistry and Biotechnology 1996; 56: 211-222 https://doi.org/10.1007/BF02786953
  13. Munford JE. A review of anatomical and biochemical changes in the mammary gland with particular reference to quantitative methods of accessing mammary development. J Dairy Sci Abstr. 1964; 26: 293
  14. Nchare A, Salaheddine M, Desbuleux H, Sulon J, Beckers JF. Artificial induction of lactation in heifers aged 6 to 7 months. Annals de medicine veterinary 2001; 145: 365-372
  15. Sinh M, Kumar P. Natural induction of lactation. Indian veterinary journal 1994; 7: 405
  16. Sharma A, Kishtwaria RS, Gupta N. Artificial induction of lactation in a crossbred cow-a case report. Indian journal of veterinary medicine 2001; 21: 53
  17. Smith KL, Redman DR, Schanbacher FL. Efficiency of 17 a-estradiol and progesterone treatment to initiate lactation in infertile cows. J Dairy science 1973; 56: 657
  18. Sohn BH, Kim SJ, Park HG, Park SK, Lee CS, Hong HJ, Park YS, Lee KK. Expression and characterization of bioactive Human Thrombopoietin in the milk of transgenic mice. DNA and Cell Biology 1999; 18: 845-482 https://doi.org/10.1089/104454999314845
  19. Verma HK, Takkar OP, Pangaonkar GR, Sidhu SS. Artificial induction of lactation in crossbred cattle. Indian journal fo dairy science 1994; 47: 912-914
  20. Willett LB, Smith KL, Schanbacher FL. Hormone induced lactation in the bovine III. Dynamics of i매 ected endogenous hormones. Purdue agricultural experiment station journal 1975; 22; 53-75
  21. 한용만, 김선정, 유대열, 박정선, 이철상, 정산균, 박인영, 손보화, 최영희, 남명수, 이훈택, 정병현, 정길생, 고대환, 김영훈, 양철서으 유욱준, 이경광. 락토레린을 우유에서 생상하는 형징 전환 젖소의 개발에 관한 연구. 한국가축번식학회지 1996; 20; 371-378