Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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The Oxidative Stress Induction and Response of Antioxidative Enzymes in the Large Patch-Infected Zoysiagrass II. Activity of antioxidative enzymes

라지 팻치에 감염된 잔디의 산화적 스트레스 발현과 항산화효소의 활력의 변화 II. 항산화효소의 활력

  • Kim, Dae-Hyun (Chonnam National University) ;
  • Lee, Bok-Rye (Chonnam National University) ;
  • Li, Ming (Chonnam National University) ;
  • Kim, Tae-Hwan (Department of Animal Science & Institute of Agriculture Science and Technology, College of Agriculture & Life Science, Chonnam National University)
  • Published : 2007.06.30
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Abstract

To investigate the effect of large patch infection on oxidative stress induction, super-oxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were compared between pathogen-infected and healthy (control) zoysiagrass. The sampling for leaves and roots were carried out every 2 days for a period of 6 days. The SOD activity was not significantly affected by pathogen-infection until day 2, but significant increase of both leaves (+48%) and roots (+49%) were observed at day 6 compared with control. The CAT activity was remarkedly increased by +25% in leaves and +101% in roots within the first 2 days and then rapidly decreased. The POD activity in pathogen-infected leaves was significantly increased by 74% at day 6. The increase of POD activity in pathogen-infected roots was 2-fold higher than that of the control at day 6. These results indicated that large patch-infection induce oxidative stress, and that SOD-CAT-POD antioxidant system of zoysiagrass was effectively operated.

한국형 잔디에 있어 병원성 (라지 팻치) 감염이 산화적 스트레스의 발현에 미치는 영향을 규명하기 위하여 라지 팻치에 감염된 잔디의 항산화 효소의 활성을 조사하였다. 처리 후 6일 동안 이틀간격으로 잎과 뿌리 시료에 대해 각각 분석하였다. 초기 2일 동안 SOD효소 활성은 처리 간 아무런 차이가 나타나지 않았으나 처리 후 6일차에는 대조구에 비해 라지 팻치에 감염된 잎에서 48% 뿌리에서 49% 각각 높게 나타났다. CAT 효소활성은 처리 후 2일간 잎에서 25% 뿌리에서 101% 각각 증가하였지만 그 이후 급격히 감소하여 4일차에는 대조구에 비해 현저히 낮게 나타났다. 라지 팻치에 감염된 잎에서 POD 활성은 감염 기간 동안 현저히 증가하여 6일 차에는 75% 까지 증가하였다. 뿌리에서 감염에 따른 POD 효소 활성의 증가는 뚜렷하였으며 대조구에 비해 약 2배 높았다. 이러한 결과들은 잔디에 있어 라지 팻치 감염은 산화적 스트레스를 유도하며, 이에 따라 초기 6일간의 병원성 감염에 대한 SOD-CAT-POD 항산화적 기작이 효과적으로 작동됨을 보여준다.

Keywords

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