Antioxidative Activity and Nitrite Scavenging Ability of Methanol Extract from Salvia plebeia R. Br.

배암차즈기 메탄올 추출물의 항산화 활성 및 아질산염 소거능

  • Lim, Jin-A (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang Uniersity) ;
  • Yun, Bo-Won (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang Uniersity) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang Uniersity)
  • 임진아 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 윤보원 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2007.06.30

Abstract

Efficacy of antioxidative activity and nitrite scavenging ability of methanol extract from Salvia plebeia was investigated. Electron-donating ability of extract at RC$_{50}$ was 51.10 ${\mu}$g/ml. After addition of 0.92 mg/ml extract, autooxidation of pyrogallol decreased to 62.11% by superoxide dismutase-like activity. In antioxidative activity of extract against linoleic acid during incubation times of 1,2, and 4 days at 40$^{\circ}$, lipid peroxidation values significantly decreased by 72.86%, 77.18%, and 78.32% with addition of 0.2 mg/ml, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and values revealed as 202.35${\pm}$ 1.26 GAE ${\mu}$g/ml of extract. Nitrite scavenging ability showed the most remark-able effect at pH 1.2, exhibited to 44.29% by addition of 0.2 mg/ml. These results suggest that methanol extract from Salvia plebeia can be used as bioactive and functional materials.

본 연구는 배암차즈기 메탄을 추출물의 생리활성 및 기능성을 검토하기 위해서 항산화효과 및 아질산염 소거능을 측정하였다. 그 결과, 배암차즈기 메탄을 추출물의 전자공여능(RC$_{50}$)은 51.10 ${\mu}$g/ml로 나타났고 SOD 유사활성은 추출물(0.92mg/ml)을 첨가하였을때 62.11%로 가장 높게 관찰되었으며, linoleic acid에 대한 항산화력은 추출물 (0.2mg/ml)을 첨가하여 지질과산화물을 측정한 결과 배양시간 1, 2, 4일 경과후 각각 72.86%, 77.18%, 78.32% 감소율을 보임으로써 효과적인 항산화 효과를 나타내었다. 총 페놀함량은 gallic acid equivalents (GAE)로 측정되었고, 202.35${\pm}$ 1.26 GAE ${\mu}$ extract으로 조사되었다. 아질산염 소거능은 복합 생약추출물(0.2mg/ml)을 첨가하였을 때 pH 1.2조건에서 44.29%로 가장 높게 나타났으며 pH가 증가할수록 감소하였다. 이상의 결과로 배암차즈기 메탄을 추출물은 우수한 항산화 효과와 아질산염 소거능을 나타냄으로써 기능성 식품 소재 및 식품 첨가물로써 사용가능 하리라 사료된다.

Keywords

References

  1. An BJ, Park JM, Bae HJ, Pyun JR Song MA (2006) Antioxidant and antibacterial effects of Koream Isodon japonicus H. J. Korean Soc. Appl. Biol. Chem. 49(2):129-134
  2. Bartsh H, Ohshima H, Pignatell B (1998) Inhibition of endogenous nitrosation: Mechanism and implications in human cancer prevention. Nut. Res. 202:307-324
  3. Blois MS (1958) Antioxidant determination by the use a stable free radical. Nature 26:1199-1200
  4. Branen AL (1975) Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52:59-63 https://doi.org/10.1007/BF02901825
  5. Choi CS, Song ES, Kim JS, Kang MN (2003) Antioxidative activities of Castanea Crenata Flos. methanol extracts. Korean J. Food Sci. Technol. 35(6):1216-1220
  6. Christiansen LN, Tompkin PB, Shaparis AB, Kueper TV, Johnston RW, Kautter DA, Kolari OJ (1974) Effect of sodium nitrite on toxin production by Chlostridium botulinum in bacon. Appl. Microbiol. 27:733-737
  7. Cooney RV, Ross PD (1987) N-Nitrosation and N-nitration of morpho line by nitrogen dioxide in aqueous solution: Effects of vanillin and related phenols. J. Agric. Food Chem. 35:789-796 https://doi.org/10.1021/jf00077a036
  8. Davy D, Gautier R (1990) New perspectives on the biochemistry of superoxide anion and the efficiency of superoxide dismutase. Biochem. Pharmacol. 39:399-405 https://doi.org/10.1016/0006-2952(90)90043-K
  9. Dinis TCP, Madeira VMC, Almeida LM (1994) Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315:161-169 https://doi.org/10.1006/abbi.1994.1485
  10. Donnelly JK, McLellan KM, Walker JL, Robinson DS (1989) Superoxide dismutase in foods: A review. Food Chem. 33:243-270 https://doi.org/10.1016/0308-8146(89)90036-8
  11. Dziezak JD (1986) Antioxidants. Food Technol. 40:94-102
  12. Fridorich I (1987) The biological activity of oxygen radicals. Science 201:875-881
  13. Gardner DR, Fridorich I (1991) Superoxide sensitivity of Escherichia coli 6-phospho gluconate dehydratase. J. Boil. Chem. 266:1478-1483
  14. Gray JI, Dugan Jr LR (1975) Inhibition of N-nitrosamine formation in model food systems. J. Food Sci. 40:981-984 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  15. Hayes RE, Bookwalter GN, Bagley EB (1997) Antioxidant activity of soybean flour and derivatives. J. Food Sci. 6:1527-1532
  16. Heo JC, Park JY, An SM, Lee JM, Yun CY, Shin HM, Kwon TK, Lee SH (2006) Anti-oxidant and anti-tumor activities of crude extracts by Gastrodia elata Blume. Korean J. Food. Storage Circul. 13(1):83-87
  17. Imlay IA, Linn S (1986) DNA damage and oxygen radical toxicity. Science 240:1302-1309
  18. Jeong EJ (1998) Antioxidative and nitrate scavenging effects of solvent extracts from Gyrophora esculenta. Korean J. Food Nutr. 11:426-430
  19. Jung GT, Ju IO, Choi JS, Hong JS (2000) The antioxidative, antimicrobial and nitrate scavenging effects of Schizandra Chinesis RUPRECHT (Omija) seed. Korean J. Food Sci. Technol. 32:928-935
  20. Kang YR, Park YK, Lee GD (1996) The nitrite scavenging and electron donation ability of phenolic compound. Korean J. Food Sci. Technol. 28:232-239
  21. Kato H, Lee IE, Chyuen NY, Kim SB, Hayase F (1987) Inhibitory of nitrosamine formation by nondialyzable melanoidins. Agric. Biol. Chem. 51(1):1333-1338 https://doi.org/10.1271/bbb1961.51.1333
  22. Kikuzaki, H, Nakatani, N (1993) Antioxidant effects of some ginger constrtuents. J. Food Sci. 58:1407-1410 https://doi.org/10.1111/j.1365-2621.1993.tb06194.x
  23. Kim DS, Ahn BW, Yeum DM, Lee DH, Kim SB, Park YH (1987) Degradation of carcinogenic nitrosamine formation factor by natural food components. 1. Nitrite-scavenging effects of vegetable extracts. Bull. Korean Fish. Soc. 20:463-468
  24. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR (2004) Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol. 36(2):333-338
  25. Kim SB, Ahn BW, Yeum DM, Lee DH, Park YH, Kim DS (1987) Degradation of carcinogenic nitrosamine formation factor by natural food components. 2. Nitrite-scavenging effects of seaweed extracts. Bull. Korean Fish Soc. 20:469-475
  26. Kim SJ, Han DS, Moon KD, Rhee JS (1995) Measurement of superoxide dismutase-like activity of natural antioxidants. Biosci. Biotechnol. Biochem. 59:822-826 https://doi.org/10.1271/bbb.59.822
  27. Korycka-Dahl M, Richardson T, Hicks CL (1979) Superoxide dismutase activity in bovine milk serum. J. Food Prot. 42:867-871 https://doi.org/10.4315/0362-028X-42.11.867
  28. Kwak CS, Lim SJ, Kim SA, Park SC, Lee MS (2004) Antioxidative and antimutagenic effects of korean buckwheat, sorghum, millet and jpb's tears. J. Korean Soc. Food Sci. Nutr 33(6):921-929 https://doi.org/10.3746/jkfn.2004.33.6.921
  29. Macrae R, Robinson RK, Sadler MJ (1993) Encyclopedia of food science food technology and nutrition. Academic Press, New York, NY, USA. 3240-3249
  30. Leaf CD, Vecchio AL, Roe DA, Hotchkiss JH (1987) Influence of ascorbic acid dose on N-nitrosoproline formation in humans. Carcinogenesis 791-795 https://doi.org/10.1093/carcin/8.6.791
  31. Lee HJ, Han DS (2002) Appliances and Antitoxic Effects of the Extracts of Salviae Plebeise R. BR..원광치의학. 11(2):199-216
  32. Lee GD, Chang HG, Kim HK (1997) Antioxidative and nitrite scavenging activities of edible mushrooms. Korean J. Food Sci. Technol. 29:432-436
  33. Lee JH, Lee SR (1994) Analysis of phenolic substance content in Korean plant foods. Korean J. Food Sci. Thechnol. 26:310-316
  34. Lee SE, Son DW, Yoon YP, Lee SY, Lee BJ, Lee SH (2006) Protective effect of the methanol extracts of Acanthopanax koreanum against oxidative stress. Kor. J. Pharmacogn. 37(1):16-20
  35. Lim JA, Na YS, Baek SH (2004) Antioxidant activity and nitrite scavenging ability of ethanol extract from Phyllostachys bambusoides. Korean J. Food Sci. Technol. 36(2):306-310
  36. Mang YS, Park HK( (1991) Antioxidant activity of ethanol extract from Dodok (Codonopsis lanceolatdi. Korean J. Food Sci. Technol. 23:311-316
  37. Marklund S, Marklund G (1974) Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47:468-474
  38. Mirvish SS (1970) Kinetics of dimethylamine nitrosation in relation to nitrosamine carcinogenesis. J. Natl. Cancer Inst. 44:633-639
  39. Mirvish SS (1975) Formation of N-nitro compounds: Chemistry, kinetics and in vivo oxxurrence. Toxicol. Appl. Pharmacol. 31:325-351 https://doi.org/10.1016/0041-008X(75)90255-0
  40. Ra KS, Suh HJ, Chung SH, Son JY (1997) Antioxidant activity of solvent extract from onion skin. J. Food Sci. Technol. 29:595-600
  41. Shin MK, Kim SK, Lee SK, Yang EY, Lee HO, Baek SH (2001) Cytotoxicity and antimicrobial effect of the extract of Salvia plebeian. Kor. J. Pharmacogn. 32(1):55-60
  42. Slinkard K, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. Am. J. Enology and Viticulture 28:49-55
  43. Sung KC (2006) A study on the pharmacetical characteristics & analysis of Glycyrrhizin Extract. J. Korean Oil Chemists' Soc. 23(3):215-222