Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Hepatoprotective Constituents of Saururus chinensis Roots Against Tacrine-induced Cytotoxicity in Human Liver-derived Hep G2 Cells

삼백초근의 타크린으로 유발한 간 세포독성 보호 성분

  • Jeong, Gil-Saeng (X-ray Microscopy Research Center, Wonkwang University) ;
  • Li, Bin (College of Pharmacy, Wonkwang University) ;
  • Lee, Dong-Sung (College of Pharmacy, Wonkwang University) ;
  • Kwon, Ji-Wung (College of Life Sciences and Natural Resources, Wonkwang University) ;
  • Lee, Hye-Suk (College of Pharmacy, Wonkwang University) ;
  • Kwon, Tae-Oh (College of Life Sciences and Natural Resources, Wonkwang University) ;
  • Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
  • 정길생 (원광대학교 X-선현미경연구센터) ;
  • 리빈 (원광대학교 약학대학) ;
  • 이동성 (원광대학교 약학대학) ;
  • 권지웅 (원광대학교 생명자원대학) ;
  • 이혜숙 (원광대학교 약학대학) ;
  • 권태오 (원광대학교 생명자원대학) ;
  • 김윤철 (원광대학교 약학대학)
  • Published : 2007.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Five liglans, sauchinone (1), di-0-methyltetrahydrofuriguaiacin B (2), manassantin A (3), manassantin B (4) and saucerneol B (5), have been isolated from the MeOH extract of Saururus chinesis roots. The evaluation for protective effect of compounds 1-5 against tacrine-induced cytotoxicity in human liver-derived Hep G2 cells was conducted. Compounds 1,2, and 5 showed significant protective effects with the EC$_{50}$ values of74.2${\pm}$0.9, 111.3${\pm}$0.8,64.3${\pm}$0.8 ${\mu}$M, respectively. Silybin, one of the well-known hepatoprotective agents, used as a positive control, and also showed protective effect with an EC$_{50}$ value of 86.2${\pm}$0.5 ${\mu}$M.

Keywords

References

  1. Jim, L. K. and Gee, J. P. (1995) Adverse effects of drugs on the liver. In Young, L. Y and Koda-Kimble, M. A. (ed.), Applied therapeutics: The clinical use of drugs, 26-1-26-17, Applied Therapeutics, Inc., Vancouver
  2. Watkins, P. B., Zimmermann, H. J., Knapp, M. J., Gracon, S. I. and Lewis, K. W. (1994) Hepatotoxic effects of tacrine administration in patients with Alzheimer's disease. J. Am. Med. Assoc. 271: 992-998 https://doi.org/10.1001/jama.271.13.992
  3. Grant, M. H., Duthie, S. J., Gray, A. G. and Burke, M. D. (1988) Mixed function oxidase and UDP-glucuronyltransferase activities in the human Hep G2 hepatoma cell line. Biochem. Pharmacol. 37: 4111-4116 https://doi.org/10.1016/0006-2952(88)90103-7
  4. Viau, C. J., Curren, R. D. and Wallace, K. (1993) Cytotoxicity of tacrine and velnacrine metabolites in cultured rat, dog, and human hepatocytes. Drug Chem. Toxicol. 16: 227- 239 https://doi.org/10.3109/01480549309081817
  5. 정보섭, 신민교 (1998) 도해 향약(생약)대사전(식물편), 813-814. 도서출판 영림사, 서울
  6. Lee, W. S., Lee, D. W., Baek, Y. I., An, S. J., Cho, K. H., Choi, Y. K., Kim, H. C., Park, H. Y., Bae, K. H. and Jeong, T. S. (2004) Human ACAT-1 and -2 inhibitory activities of saucerneol B, manassantin A and B isolated from Saururus chinensis. Bioorg. Med. Chem. Lett. 14: 3109-3112 https://doi.org/10.1016/j.bmcl.2004.04.023
  7. Rho, M. C., Kwon, O. E., Kim, K., Lee, S. W., Chung, M. Y., Kim, Y. H., Hayashi, M., Lee, H. S. and Kim, Y. K. (2003) Inhibitory effects of manassantin A and B isolated from the roots of Saururus chinensis on PMA-induced ICAM-1 expression. Planta Med. 69: 1147-1149 https://doi.org/10.1055/s-2003-818007
  8. Park, H. J., Kim, R. G., Seo, B. R., Ha, J., Ahn, B. T., Bok, S. H., Lee, Y. S., Kim, H. J. and Lee, K. T. (2003) Saucernetin- 7 and saucernetin-8 isolated from Saururus chinensis inhibit the LPS-induced production of nitric oxide and prostaglandin $E_2$ in macrophage RAW 264.7 cells. Planta Med. 69: 947-950 https://doi.org/10.1055/s-2003-45106
  9. Ahn, B. T., Lee, S., Lee, S. B., Lee, E. S., Kim, J. G., Bok, S. H. and Jeong, T. S. (2001) Low-density lipoprotein-antioxidant constituents of Saururus chinensis. J. Nat. Prod. 64: 1562-1564 https://doi.org/10.1021/np0006061
  10. Song, E. K., Cho, H., Kim, J. S., Kim, N. Y., An, N. H., Kim, J. A., Lee, S. H. and Kim, Y. C. (2001) Diarylheptanoids with free radical scavenging and hepatoprotective activity in vitro from Curcuma longa. Planta Med. 67: 876-877 https://doi.org/10.1055/s-2001-18860
  11. Sung, S. H. and Kim, Y. C. (2000) Hepatoprotective diastereomeric lignans from Saururus chinensis herbs. J. Nat. Prod. 63: 1019-1021 https://doi.org/10.1021/np990499e
  12. Sung, S. H., Huh, M. S. and Kim, Y. C. (2001) New tetrahydrofuran- type sesquilignans of Saururus chinensis root. Chem. Pharm. Bull. 49: 1192-1194 https://doi.org/10.1248/cpb.49.1192
  13. Rao, K. V. and Alvarez, F. M. (1983) Manassantins A/B and saucerneol: novel biologically active lignoids from Saururus cernuus. Tetrahedron Lett. 24: 4947-4950 https://doi.org/10.1016/S0040-4039(01)99818-1
  14. Rao, K. V. and Oruganty, R. S. (1997) An improved method for the isolation of the lignan constituents of Saururus cernuus by reverse phase column chromatography. J. Liq. Chrom. & Technol. 20: 3121-3134 https://doi.org/10.1080/10826079708000478
  15. Galisteo, M., Rissel, M., Sergent, O., Chevanne, M., Cillard, J., Guillouzo, A. and Lagadic-Gossmann, D. (2000) Hepatotoxicity of tacrine: occurrence of membrane fluidity alterations without involvement of lipid peroxidation. J.Pharmacol. Exp. Ther. 294: 160-167
  16. Sung, S.H., Lee, E.J., Cho, J.H., Kim, H.S. and Kim, Y.C. (2000) Sauchinone, a lignan from Saururus chinensis, attenuates CCl4-induced toxicity in primary cultures of rat hepatocytes. Biol. Pharm. Bull. 23: 666-668 https://doi.org/10.1248/bpb.23.666