Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Strain Development by Electrofusion between Phytase-producing Yeast and Salt-tolerant Yeast

전기융합법을 이용한 phytase 생성 내염성 효모 균주의 개발

  • Oh, Chul-Hwan (Department of Food Science and Technology, Kongju National University) ;
  • In, Man-Jin (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • Oh, Nam-Soon (Department of Food Science and Technology, Kongju National University)
  • Published : 2007.03.31
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Abstract

내염성 조건에서 phytase를 생성하는 효모 균주를 개발하기 위하여 phytase를 생산하는 효모인 S. cerevisiae CY 균주와 내염성 효모인 Z. rouxii Y-80 균주 사이의 전기적 세포융합을 실시하였다. S. cerevisiae CY 균주의 Z. rouxii Y-80 균주의 배양된 세포를 1.0%의 ${\beta}$-mercaptoethanol로 전처리하고 Tunicase로 세포벽을 분해시킨 후 원형질체를 얻었다. 각 균주의 원형질체는 electrofusion 완충액에 1:1의 비율로 현탁한 후 AC 13V에서 30초간 처리하고 고전압(DC 500 V/100 ${\mu}sec$)을 가하여 전기적 세포융합을 실시하였다. 선별된 융합균주는 NaCl이 10% 첨가된 배지에서 친주들과 비교한 결과 Z. rouxii Y-80 균주보다는 생육면에서 25% 이상 증가하였으며, phytase 활성도는 친주인 S. cerevisiae CY 균주 대비 53%의 생성도를 확인하였다.

Keywords

References

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