Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Development of Biologically Active Compounds from Edible Plant Sources XVIII. Isolation of Derivatives of Ergosterol from the Fruit Body of Phellinus linteus

식용 식물자원으로부터 활성물질의 탐색-XVIII. 상황버섯 (Phellinus linteus) 자실체로부터 Ergosterol 유도체의 분리

  • Lyu, Ha-Na (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Yoo, Jong-Su (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Song, Myoung-Chong (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Dae-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Kim, Dong-Hyun (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Rho, Young-Duk (College of Life Science, Institute of Life Science & Resources, Kyung Hee university) ;
  • Kim, In-Ho (Korea Food Research Institute) ;
  • Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
  • 류하나 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 유종수 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이대영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김동현 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 노영덕 (경희대학교 생명자원과학연구원) ;
  • 김인호 (한국식품연구원) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2007.03.31
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Abstract

The fruiting body of Phellinus linteus was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2$O. The repeated silica gel and ODS column chromatographies of the EtOAc fraction led to isolation of four sterols. From the result of spectral data including NMR, MS and IR, the chemical structures of the sterols were determined as ergosta-7,24(28)-dien-3${\beta}$-ol (episterol, 1), 5${\alpha}$,8${\alpha}$-epidioxyergosta-6,9(11),22-trien-3${\beta}$-ol (dehydrop-eroxyergosterol, 2), 5${\alpha}$,8${\alpha}$-epidioxyergosta-6,22-dien-3${\beta}$-ol (ergoterol peroxide, 3), and $3{\beta}$,$5{\alpha}$-dihydroxy-6${\beta}$-methoxyergosta-7,22-diene (6-O-methylcerevisterol, 4). The ergosterols have been first isolated from this mushroom in this study.

상황버섯 자실체를 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc 분획을 silica gel 및 octadecylsilica gel(ODS) column chromatography로 정제하여 4종의 sterol 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ergosta-7,24(28)-dien-3${\beta}$-ol (episterol, 1), 5${\alpha}$,8${\alpha}$-epidioxyergosta-6,9(11),22-trien-3${\beta}$-ol (dehydroperoxyergosterol, 2), 5${\alpha}$,8${\alpha}$-epidioxyergosta-6,22-dien-3${\beta}$-ol (ergoterol peroxide, 3), 및 $3{\beta}$,$5{\alpha}$-dihydroxy-6${\beta}$-methoxyergosta-7,22-diene (6-O-methylcerevisterol, 4)로 동정하였다. 이 화합물들은 이번에 상황에서 처음 분리 보고되었다.

Keywords

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