Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Cha-ga Mushroom Water Extract induces G0/G1 Arrest in B16-F10 Melanoma cells

차가버섯추출물에 의한 흑색종의 세포주기 억제효과

  • Youn, Myung-Ja (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University School of Medicine) ;
  • Song, Jeong-Hoon (Plastic & Reconstructive Surgery, Wonkwang University School of Medicine)
  • 윤명자 (원광대학교 의과대학 전정와우센터) ;
  • 송정훈 (원광대학교 의과대학 성형외과학교실)
  • Published : 2007.02.25
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Abstract

Chaga mushroom extract is well known as immune modulator and anti-cancer agent. However, the molecular mechanism by which Chaga exerts cell cycle arrest and apoptosis of cancer cells is poorly understood. In this study, we demonstrated anti-proliferative effects of Chaga extract on murine melanoma B16 cells. Chaga extract dose-dependently inhibited cell growth along with the arrest of G0/G1 phase and the induction of apoptotic cell death. Treatment with Chaga extract resulted in a decrease of cyclin E, cyclin D1, cdk 2, cdk 4 expression levels. Furthermore, in vivo inoculation study of B16 melanoma cells into Balb/c mice Chaga extract markedly suppressed the metastatic growth of tumor cells (6 folds, p<0.05,). These results indicate that Chaga mushroom extract induces apoptosis of B16 melanoma cells through arrest of G0/G1 phase in cell cycle.

Keywords

References

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