Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effects of Bojungbangam-tang Extracts on ECV304 Cell Cytotoxicity

보정방암탕 추출물의 혈관내피세포독성에 대한 방어효과

  • Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Eun-Kyung (Department of Biochemistry, Chonbuk National University Medical School) ;
  • Song, Mi-Young (Department of Biochemistry, Chonbuk National University Medical School) ;
  • Han, Mi-Jeong (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Su-Yeop (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Heon-Jae (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Young-Rae (Department of Biochemistry, Chonbuk National University Medical School) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu, Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Sung-Hoon (Department of Pathology, College of Oriental Medicine, KyungHee University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김은경 (전북대학교 의과대학 생화학교실) ;
  • 송미영 (전북대학교 의과대학 생화학교실) ;
  • 한미정 (원광대학교 한의과대학 생리학교실) ;
  • 이수엽 (원광대학교 한의과대학 생리학교실) ;
  • 이헌재 (원광대학교 한의과대학 생리학교실) ;
  • 이영래 (전북대학교 의과대학 생화학교실) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실) ;
  • 김성훈 (경희대학교 한의과대학 병리학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Published : 2007.04.25
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Abstract

This study was designed to investigate the protective effect of Bojungbangam-tang Ethanol Extracts (EBJT) on cisplatin and hydrogen peroxide-induced cytotoxicity of human endothelial cell line ECV304 cells. After cells were treated with cisplatin and hydrogen peroxide, MTT assay was performed for cell viability test. To explore the mechanism of cytotoxicity, we used the several measures of apoptosis to determine whether this processes was involved in cisplatin and hydrogen peroxide-induced cell damage in ECV304 cells. Also, cells were treated with EBJT and then, followed by the addition of cisplatin or hydrogen peroxide. Cisplatin or hydrogen peroxide decreased the viability of ECV304 cells in a dose-dependent manner. ECV304 cells treated cisplatin or hydrogen peroxide were revealed as apoptosis characterized by nuclear staining. EBJT protected ECV304 cells from cisplatin or hydrogen peroxide-induced nuclear fragmentation and chromatin condensation. Also, EBJT inhibited the cleavage of poly(ADP-ribose) polymerase (PARP) in cisplatin or hydrogen peroxide-treated ECV304 cells. According to above results, EBJT may protect ECV304 cells from the apotosis induced by cisplatin or hydrogen peroxide.

Keywords

References

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