Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibition of Browning in Yam Fresh-cut and Control of Yam-putrefactive Bacterium Using Acetic Acid or Maleic Acid.

초산 및 말레산을 이용한 생마 신선편이 갈변억제 및 생마 저온부패균의 제어

  • Ryu, Hee-Young (Department of Food and Nutrition, Andong National University) ;
  • Kwun, In-Sook (Department of Food and Nutrition, Andong National University) ;
  • Park, Sang-Jo (Experiment Station of Bioresources, Gyeongbuk Agricultural Technology Administration) ;
  • Lee, Bong-Ho (Experiment Station of Bioresources, Gyeongbuk Agricultural Technology Administration) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
  • 류희영 (안동대학교 식품영양학과) ;
  • 권인숙 (안동대학교 식품영양학과) ;
  • 박상조 (경북농업기술원 생물자원연구소) ;
  • 이봉호 (경북농업기술원 생물자원연구소) ;
  • 손호용 (안동대학교 식품영양학과)
  • Published : 2007.06.28
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Abstract

To increase the consumer acceptability of yam and the shelf-life of fresh-cut yam, organic acid-treated fresh-cut yam was prepared. When uncontaminated fresh-cut yam was stored at $4^{\circ}C$ for 14 days after treatment with 1% (v/w) organic acids, the browning and microbial putrefaction of fresh-cut yam were inhibited by treatment of acetic acid or maleic acid, whereas treatment of citric acid and ascorbic acid, commonly used browning inhibitors in food industry, did not show apparent effects on the browning and putrefaction of yam. The Inhibitory effects of acetic acid or maleic acid were superior than those of NaOCl (100 ppm), hydrogen peroxide (100 ppm) or commercially available washing solution. Also, treatments of 1% acetic acid, or 1% maleic acid Into artificially-contaminated yam $(10^5\;CFU/g-yam)$ showed strong inhibition of browning and putrefaction during long term storage at $4^{\circ}C$. The growth inhibition test indicated that 0.1% is enough to inhibit the growth of psychrotrophic yam-putrefactive Pseudomonas sp., and treatment of 0.1% acetic acid, or 0.1% maleic acid inhibited the browning and microbial putrefaction of fresh-cut yam. Our results suggested long-term distribution of yam or other root crops products is possible by treatment of organic acid, such as acetic acid, combined with aseptic vacuum packaging technology.

생마의 이용성과 저장성을 증대시키기 위해, 유기산을 처리한 생마 신선편이 제품을 제조하였다. 저온 부패미생물의 초기오염이 없는 생마 신선편이의 경우, 1% 농도의 초산, 구연산, 말레산 및 아스코르빈산을 각각 처리한 후 $4^{\circ}C$에서 14일간 저장한 경우, 구연산 및 아스코르빈산 처리구에서는 갈변 및 부패가 유의적으로 진행되었으나, 초산 및 말레산 처리구에서 우수한 갈변 및 부패 억제 활성이 나타났다. 이러한 억제활성은 기존에 사용되고 있는 NaOCl(100 ppm), 과산화수소수(100 ppm) 및 1종 세척제(L사, 한국)처리보다 우수하였으며, 특히 초기 부패미생물이 $10^5\;CFU/g-yam$ 이상 오염된 신선편이의 경우에도 초산 및 말레산은 강력한 부패억제 및 갈변억제효과를 나타내었다. 생마의 저온 부패미생물(Pseudomonas rhodesiae YAM-12 및 P. cepacia YAM-10)을 대상으로 초산 및 말레산의 최소처리농도를 조사한 결과 $4^{\circ}C$에서는 0.1% 처리로 우수한 항균력이 나타남을 확인하였으며, 실제 오염되지 않은 신선편이의 경우 0.1% 처리로 유의적인 갈변 및 부패현상 없이 14일간 저온저장이 가능하였다. 이러한 결과는, 초기 저온 부패미생물의 오염이 심각하지 않은 신선편이의 경우에는, 0.1% 초산 및 말레산 처리로 $4^{\circ}C$에서 14일 장기저장이 가능함을 나타내며, 적절한 진공포장 등이 병행된다면 생마 및 근채류 신선편이 농산물의 장기간 저온 유통이 가능할 것으로 판단된다.

Keywords

References

  1. Ahn J.-H., K. H. Son, H. Y. Sohn, and S. T. Kwon. 2005. In vitro culture of adventitious roots from Dioscorea nipponica Makino for the production of steroidal saponins. Kor. J. Plant Biotechnol. 32: 217-223 https://doi.org/10.5010/JPB.2005.32.3.217
  2. Hong, S. I., S. M. Son, M. S. Chung, and D. Kim. 2003. Storage quality of minimally processed onions as affected by seal-packaging methods. Kor. J. Food Sci. Technol. 35: 1110-1116
  3. Hwang, J. B., D. B. Shin, and Y. C. Lee. 2003. The inhibition of green discoloration in garlic by conditioning. Kor. J. Food. Sci. Technol. 35: 1007-1016
  4. Kang, K. J., G. S. Oh, Y. S. Go, I. W, Seo, Y. J. Kim, and D. H. Park. 2003. Inhibition of enzymatic browning in medicinal herbs(crude drug materials) by organic acid. Kor. J. Food Sci. Technol. 35: 532-535
  5. Kim, C. R., J. S. Kim, D. H. Koh, W. J. Choi., K. R. Lee. U. R. Kang, and K. H. Kim. 1998. Microbiological evaluations of refrigerated flatfish treated with organic acids. Kor. J. Food. Nutr. 11: 329-333
  6. Kim, D. M. and S. I. Hong. 2004. The current status and development of fresh-cut agricultural products. Food Storage Processing Industry. 3: 18-22
  7. Kim M. W. 2001. Effects of $H_2O$-fraction of Dioscorea japonica Thunb and selenium on lipid peroxidation in streptozotocin-induced diabetic rats. Kor. J. Soc. Food Cookery Sci. 17: 344-352
  8. Kong Y. J., B. K. Park, and D. H. Oh. 2001. Antimicrobial activity of Quercus mongolica leaf ethanol extract and organic acids against food-borne microorganisms. Kor. J. Food Sci. Technol. 33: 178-183
  9. Kubo, Y., S. Nonaka, and H. Yoshida. 1988. Allergic contact dermatitis from Dioscorea batatas Decaisne. Contact Dermatitis 18: 111-112 https://doi.org/10.1111/j.1600-0536.1988.tb02757.x
  10. Kum, E. J., S. J. Park, B. H. Lee, J. S. Kim, K. H. Son, and H. Y. Sohn. 2006. Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscroea batatas decne. J. Life Sci. 16: 647-652 https://doi.org/10.5352/JLS.2006.16.4.647
  11. Kwon, C. S., E. Y. Sohn, S. H. Kim, J. H. Kim, K. H. Son, J. S. Lee, J. K. Lim, and J. S. Kim. 2003. Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci. Biotechnol. Biochem. 67: 1451-1456 https://doi.org/10.1271/bbb.67.1451
  12. Kwon, E. G, E. M. Choe, and S. J. Gu. 2001. Effects of mucilage from yam (Dioscorea batatas DECENE) on blood glucose and lipid composition in alloxan-induced diabetic mice. Kor. J. Food Sci. Technol. 33: 795-801
  13. Kwon, J. Y., B. S. Kim, and G. H. Kim. 2006. Effect of washing methods and surface sterilization on quality of fresh-cut chicory. Kor. J. Food Sci. Technol. 38: 28-34
  14. Lee, B. Y. and H. K. Kim. 1998. Quality properties of korean yam by various drying methods. Kor. J. Food Sci. Technol. 30: 877-882
  15. Lim, J. H., J. H. Choi, S. I. Hong, M. C. Jeong, and D. Kim. 2005. Quality changes of fresh-cut potatoes during storage depending. on the packaging treatments. Kor. J. Food Sci. Technol. 37: 933-938
  16. Park H. S., M. J. Kim, and H. B. Moon. 1994. Occupational asthma caused by two herb materials, Dioscorea batatas and Pinellia ternata. Clin. Exp. Allergy 24: 575-581 https://doi.org/10.1111/j.1365-2222.1994.tb00955.x
  17. Ryu, H. Y., Y. S. Kim, S. J. Park, B. H. Lee, S. T. Kwon and H. Y. Sohn. 2006. Isolation and characterization of yam-putrefactive psychrotrophic bacteria from rotted yam. Kor. J. Microbiol. Biotechnol. 34: 109-114
  18. Ryu, H. Y., S. J. Park, B. H. Lee, and H. Y. Sohn. 2007. Control of yam putrefactive psychrotrophic bacterium using clove oil and preparation of functional Fresh-cut. Kor. J. Microbiol. Biotechnol. 35: 66-72
  19. Shin, S. R. 2004. Changes on the components of yam snack by processing methods. Kor. J. Food Preservation 11: 516-521
  20. Stohs, J. S., C. L. Wegner, and H. Rosenberg. 1975. Steroids and sapogenins of Dioscorea deltoidea tissue cultures. Planta Med. 28: 101-105 https://doi.org/10.1055/s-0028-1097836