Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 39 Issue 3
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- Pages.249-255
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- 2007
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- 1738-3544(pISSN)
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- 2288-1662(eISSN)
Mechanism of Glucose Uptake on PMA Stimulated Neutrophils
PMA에 의한 중성구의 당섭취 기전 연구
- Paik, Jin-Young (Department of Nuclear Medicine, Samsung Medical Center) ;
- Ko, Bong-Ho (Department of Nuclear Medicine, Samsung Medical Center) ;
- Yoo, Man-Kil (Department of Nuclear Medicine, Samsung Medical Center) ;
- Jin, Kwang-Ho (Department of Nuclear Medicine, Samsung Medical Center)
- Published : 2007.12.31
Abstract
While respiratory burst enhances neutrophil glucose utilization, many neutrophil functions are critically influenced by extracellular matrix interaction and phosphoinositide-3-OH kinase (PI3K) signaling. We thus evaluated the role of RGD integrin occupancy and PI3K inhibition on respiratory burst and [18F]FDG uptake of stimulated neutrophils. Human neutrophils were stimulated by 100 ng/mL phorbol-myristate-acetate (PMA), and respiratory burst was measured by cumulative luminescence with lucigenin. [18F]FDG uptake and total hexokinase activity was measured 20 min after PMA stimulation in the presence or absence of soluble RGD peptides (200 g/mL) and/or the PI3K inhibitor wortmannin (200 nM). PMA induced a 71.70.9 fold increase in neutrophil oxygen intermediate generation. [18F]FDG uptake was increased to