Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 39 Issue 3
- /
- Pages.241-248
- /
- 2007
- /
- 1738-3544(pISSN)
- /
- 2288-1662(eISSN)
Correlation Analysis of Apoptosis and Cell Proliferating Marker (Ki-67) in Thyroid Tumors
갑상선 종양에서 아포프토시스(Apoptosis)와 세포증식 표지자(Ki-67)의 상관관계 분석
- Han, Kyung-Hee (Department of Pathology, Yonsei University Collage of Medicine) ;
- Yang, Woo-Ick (Department of Pathology, Yonsei University Collage of Medicine) ;
- Kim, Sun-Jung (Department of Life Science, Dongguk University) ;
- Kim, Tai-Jeon (Department of Biomedical Laboratory Science, College of Health Sciences, Eulji University)
- Published : 2007.12.31
Abstract
To clarify the growth mechanisms of thyroid tumors, we investigated apoptotic cells in 88 thyroid tumors, consisting of 24 adenomas, 58 papillary thyroid carcinomas, and 6 undifferentiated carcinoma, using terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate digoxigenin-nick end labeling (TUNEL). The cell proliferating marker was also evaluated immunohistochemically using the monoclonal antibody to Ki-67 antigen (MIB-1) in the same tumors. The apoptosis was expressed as a percentage of the TUNEL-positive cells in the tumor cells, and a proliferating marker, being the percentage of Ki-67 positive cells, was counted up each tumor. The statistical analysis were used analysis of variance (ANOVA) and student's t-test that were analyze the differences in the rate of each histological types of the thyroid tumors. The overall level of apoptosis was extremely low in all histological types of the thyroid tumors analyzed, the mean apoptosis being