Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Induction of osteoclastogenesis-inducing cytokines and invasion by alive Aggregatibacter actinomycetemcomitans in osteoblasts

조골세포에서 Aggregatibacter actinomycetemcomitans 생균의 파골세포분화유도 cytokine 발현 유도능 및 침투능

  • Choi, Ho-Kil (Department of Oral Biology, Brain Korea 21 project, College of Dentistry, Yonsei University) ;
  • Lee, Yang-Sin (Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Kim, Min-Young (Department of Oral Biology, Brain Korea 21 project, College of Dentistry, Yonsei University) ;
  • Kim, Kyoung-Dae (Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Cha, Jeong-Heon (Department of Oral Biology, Brain Korea 21 project and Oral Science Research Center, College of Dentistry, Yonsei University) ;
  • Yoo, Yun-Jung (Department of Oral Biology, Brain Korea 21 project and Oral Science Research Center, College of Dentistry, Yonsei University)
  • 최호길 (연세대학교 치과대학 구강생물학교실 및 2단계 BK21사업) ;
  • 이양신 (연세대학교 치과대학 구강생물학교실) ;
  • 김민영 (연세대학교 치과대학 구강생물학교실 및 2단계 BK21사업) ;
  • 김경대 (연세대학교 치과대학 구강생물학교실) ;
  • 차정헌 (연세대학교 치과대학 구강생물학교실, 2단계 BK21 사업 및 구강과학연구소) ;
  • 유윤정 (연세대학교 치과대학 구강생물학교실, 2단계 BK21 사업 및 구강과학연구소)
  • Published : 2007.09.30
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Abstract

Osteoblasts regulate osteoclastogenesis by production of various cytokines. Aggregatibacter(A) ac-tinomycetemcomitans is one of periodontopathogens which invades gingival tissue. Therefore, clarifying the effect of alive A. actinomycetemcomitans on osteoblasts is important to understand the mechanism of alveolar bone resorption in periodontitis. We investigated induction of osteoclastogenesis-inducing cytokines, adherence, and invasion by A. actinomycetemcomitans in osteoblasts. Osteoblasts were isolated from mouse calvaria and expression of cytokines was determined by RT-PCR. When the ratio of the number of A. actinomycetemcomtians to the number of osteoblasts was 10:1, 50:1 and 100:1, RANKL mRNA expression was increased. A. actinomycetemcomitans also increased expression of macrophage inflammatory protein (MIP) -1${\alpha}$, interleukin (IL)-1${\beta}$, and tumor necrosis factor (TNF)-${\alpha}$. A. actinomycetemcomitans attached to and invaded osteoblasts at ratio of 1000:1. These results suggest that A. actinomycetemcomitans increases osteoclastogenesis-inducing ability of osteoblasts by stimulating the expression of RANKL, MIP-1${\alpha}$,IL-1${\beta}$, and TNF-${\alpha}$ and that invasion of A. actinomycetemcomitans provides a means by which the bacteria escape from immune system and antibiotic therapy.

Keywords

References

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