Journal of Periodontal and Implant Science

  • 제37권3호
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  • Pages.511-522
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  • 2007
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

재조합 골형성 단백질 2형(rh-BMP-2) 함유 무세포성 진피조직(acellualr dermal matrix)의 골재생 효과

Effects of Recombinant Human Bone Morphogenetic Protein-2 loaded Acellular Dermal Matrix on Bone Formation

  • 송대석 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 김태균 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 정의원 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 최성호 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 조규성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 채중규 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 김종관 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 김창성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소)
  • Song, Dae-Seok (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Kim, Tae-Gyun (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Jung, Ui-Won (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Choi, Seong-Ho (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Cho, Kyoo-Sung (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Chai, Jung-Kiu (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Kim, Chong-Kwan (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Kim, Chang-Sung (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University)
  • 발행 : 2007.09.30
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초록

Introduction : The purpose of this study was to evaluate the possibility of the acellular dermal matrix (ADM) as a barrier membrane for bone regeneration, and to evaluate the osteogenic effect of ADM as a carrier system for rhBMP-2 in the rat calvarial defect model. Materials and Methods: An 8-mm, calvarial, critical-size osteotomy defect was created in each of 60 male Spraque-Dawley rats(weight $250{\sim}300g$). Three groups of 20 animals, each received either rhBMP-2(0.025mg/ml) in an ADM carrier, ADM only, or negative surgical control. And each group was divided into 2- and 8-weeks healing intervals. The groups were evaluated by histologic and histomorphometric parameters(10 animals/group/healing intervals). Data were expressed as $means{\pm}standard$ deviations($m{\pm}SD$). Comparisons between experimental and control groups were made using two-way ANOVA and post hoc t-test. Comparisons between 2 weeks and 8 weeks were made using paired t-test. The level of statistical difference was defined as P< 0.05. Results : The ADM group and rhBMP-2/ADM group results in enhanced local bone formation in the rat calvarial defect at both 2 and 8 weeks. The amount of defect closure and new bone formation were significantly greater in the rhBMP-2/ADM group relative to ADM group(P<0.05). At 8 weeks, the majority of ADM in the defect was contracted, and integrated with surrounding host tissues. In addition, host cell infiltration and neovascularization of the ADM in the absence of an inflammatory response were observed, and the newly formed bone around ADM showed a continuous remodeling and consolidation. Conclusion : The results of the present study indicated that ADM may be used as a barrier membrane for bone regeneration and that may be employed as a delivery system for BMPs.

키워드

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