Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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N,N-Dimethyl-D-ribo-phytosphingosine Modulates Cellular Functions of 1321N1 Astrocytes

  • Lee, Yun-Kyung (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University) ;
  • Kim, Hyo-Lim (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University) ;
  • Kim, Kye-Ok (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University) ;
  • Sacket, Santosh J. (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University) ;
  • Han, Mi-Jin (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University) ;
  • Jo, Ji-Yeong (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University) ;
  • Lim, Sung-Mee (Department of Food Science & Technology, Tongmyong University) ;
  • Im, Dong-Soon (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University)
  • Published : 2007.06.30
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Abstract

N,N-Dimethyl-D-ribo-phytosphingosine (DMPH) is an N-methyl derivative of sphingosine. In the present paper, we studied effects of DMPH on intracellular Ca$^{2+}$ concentration, pH, glutamate uptake, and cell viability in human 1321N1 astrocytes. DMPH increased intracellular Ca$^{2+}$ concentration and cytosolic pH significantly in a dose-dependent manner. DMPH also inhibited glutamate uptake by 1321N1 astrocytes. Finally, treatment of cells with DMPH for 24 h reduced viability of cells largely and concentration-dependently. In summary, DMPH increased intracellular Ca$^{2+}$ concentration and pH, inhibited glutamate uptake and evoked cytotoxicity in 1321N1 astrocytes. Our observations with DMPH in the 1321N1 astrocytes would enhance understanding of DMPH actions in the brain.

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References

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