Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic and Antigenic Characterization of Swine H1N2 Influenza Viruses Isolated from Korean Pigs

  • Jo, Su-Kyoung (Laboratory o/Influenza Research, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Hyun-Soo (Laboratory o/Influenza Research, College of Veterinary Medicine, Chungnam National University) ;
  • Cho, Sung-Whan (Laboratory o/Influenza Research, College of Veterinary Medicine, Chungnam National University) ;
  • Seo, Sang-Heui (Laboratory o/Influenza Research, College of Veterinary Medicine, Chungnam National University)
  • Published : 2007.05.31
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Abstract

H1N2 influenza viruses are circulating in pigs worldwide and cause considerable economic losses to the pig industry. We genetically analyzed the genes of our isolates from Korean pigs, and compared the antigenicity of our isolates with swine H1N2 viruses isolated from pigs in the U.S.A. In addition, we serologically surveyed the infection rate of swine H1N2 viruses in pigs. We found that H1N2 isolates from Korean pigs are genetically more related to swine H1N2 viruses isolated from pigs in the U.S.A. than those in European countries. When antigenicity was compared, our isolates were weakly reacted to antibodies against swine H1N2 viruses isolated from pigs in the U.S.A. The serological surveillance using sera from pigs in Korea showed that about 46% was positive for H1N2 viruses. Our results suggest that swine H1N2 viruses are widespread in Korean pigs, and the development of a vaccine against H1N2 viruses may help to control their infection in pigs.

Keywords

References

  1. Abe, Y., E. Takashita, K. Sugawara, Y. Katsuzaki, Y. Muraki, and S. Hongo. 2004. Effect of the addition of oligosaccharides on the biological activities and antigenicity of influenza A/H3N2 virus hemagglutinin. J. Virol. 78: 9605-9611 https://doi.org/10.1128/JVI.78.18.9605-9611.2004
  2. Brown, I. H., P. Chakraverty, P. A. Harris, and D. J. Alexander. 1995. Disease outbreaks in pigs in Great Britain due to an influenza A virus of H1N2 subtype. Vet. Rec. 136: 328-329 https://doi.org/10.1136/vr.136.13.328
  3. Brown, I. H., S. Ludwig, C. W. Olsen, C. Hannoun, C. Scholtissek, V. S. Hinshaw, P. A. Harris, J. W. McCauley, I. Strong, and D. J. Alexander. 1997. Antigenic and genetic analyses of H1N1 influenza A viruses from European pigs. J. Gen. Virol. 78: 553-562 https://doi.org/10.1099/0022-1317-78-3-553
  4. Brown, I. H., P. A. Harris, J. W. McCauley, and D. J. Alexander. 1998. Multiple genetic reassortment of avian and human influenza A viruses in European pigs, resulting in emergence of an H1N2 virus of novel genotype. J. Gen. Virol. 79: 2947-2955 https://doi.org/10.1099/0022-1317-79-12-2947
  5. Choi, C., S. K. Ha, and C. Chae. 2004. Detection and isolation of H1N1 influenza virus from pigs in Korea. Vet. Rec. 154: 274-275 https://doi.org/10.1136/vr.154.9.274
  6. Gourrean, J. M., C. Kaiser, M. Valette, A. R. Douglas, J. Labie, and M. Aymard. 1994. Isolation of two H1N2 influenza viruses from swine in France. Arch Virol. 135: 365-382 https://doi.org/10.1007/BF01310021
  7. Hu, H. B, Y. Q. Xu, F. Chen, X. H. Zhang, and B. K. Hur. 2005. Isolation and characterization of a new fluorescent Pseudomonas strain that produces both phenazine 1- carboxylic acid and pyoluteorin. J. Microbiol. Biotechnol. 15: 86-90
  8. Ito, T., J. Nelson, S. S. Couceiro, S. Kelm, L. G. Baum, S. Krauss, M. R. Castrucci, I. Donatelli, H. Kida, J. C. Paulson, R. G. Webster, and Y. Kawaoka. 1998. Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. J. Virol. 72: 7367-7373
  9. Ito, T., Y. Kawaoka, A. Vines, H. Ishikawa, T. Asai, and H. Kida. 1998. Continued circulation of reassortant H1N2 influenza viruses in pigs in Japan. Arch. Virol. 143: 1773- 1782 https://doi.org/10.1007/s007050050415
  10. Jung, K. and C. Chae. 2004. Phylogenetic analysis of an H1N2 influenza A virus isolated from a pig in Korea. Brief report. Arch. Virol. 149: 1415-1422
  11. Karasin, A. I., C. W. Olsen, and G. A. Anderson. 2000. Genetic characterization of an H1N2 influenza virus isolated from a pig in Indiana. J. Clin. Microbiol. 38: 2453- 2456
  12. Karasin, A. I., S. Carman, and C. W. Olsen. 2006. Identification of human H1N2 and human-swine reassortant H1N2 and H1N1 influenza A viruses among pigs in Ontario, Canada (2003 to 2005). J. Clin. Microbiol. 44: 1123-1126 https://doi.org/10.1128/JCM.44.3.1123-1126.2006
  13. Kida, H., T. Ito, J. Yasuda, Y. Shimizu, C. Itakura, K. F. Shortridge, Y. Kawaoka, and R. G. Webster. 1994. Potential for transmission of avian influenza viruses to pigs. J. Gen. Virol. 75: 2183-2188 https://doi.org/10.1099/0022-1317-75-9-2183
  14. Kim, J. A., S. Y. Ryu, and S. H. Seo. 2005. Cells in the respiratory and intestinal tracts of chickens have different proportions of both human and avian influenza virus receptors. J. Microbiol. 43: 366-369
  15. Kim, D., Y. K. Park, J. S. Lee, J. F. Kim, H. Jeong, B. S. Kim, and C. H. Lee. 2006. Analysis of a prodigiosin biosynthetic gene cluster from the marine bacterium Hahella chejuensis KCTC 2396. J. Microbiol. Biotechnol. 16: 1912- 1918
  16. Kundin, W. D. 1970. Hong Kong A2 influenza virus infection among swine during a human epidemic in Taiwan. Nature 228: 857
  17. Lee, J. H., Y. B. Seo, M. Yoon, J. Choi, and Y. T. Kim. 2005. Isolation and sequence analysis of two ornithine decarboxylase antizyme genes from flounder (Paralichthys olivaceus). J. Microbiol. Biotechnol. 15: 321-329
  18. Marozin, S., V. Gregory, K. Cameron, M. Bennett, M. Valette, M. Aymard, E. Foni, G. Barigazzi, Y. Lin, and A. Hay. 2002. Antigenic and genetic diversity among swine influenza A H1N1 and H1N2 viruses in Europe. J. Gen. Virol. 83: 735-745 https://doi.org/10.1099/0022-1317-83-4-735
  19. Olsen, C. W. 2002. The emergence of novel swine influenza viruses in North America. Virus Res. 85: 199-221 https://doi.org/10.1016/S0168-1702(02)00027-8
  20. Pensaert, M., K. Ottis, J. Vandeputte, M. M. Kaplan, and P. A. Bachmann 1981. Evidence for the natural transmission of influenza A virus from wild ducks to swine and its potential importance for man. Bull. World Health Org. 59: 75-78
  21. Richt, J. A., K. M. Lager, B. H. Janke, R. D. Woods, R. G. Webster, and R. J. Webby. 2003. Pathogenic and antigenic properties of phylogenetically distinct reassortant H3N2 swine influenza viruses cocirculating in the United States. J. Clin. Microbiol. 41: 3198-3205 https://doi.org/10.1128/JCM.41.7.3198-3205.2003
  22. Schultz, U., W. M. Fitch, S. Ludwig, J. Mandler, and C. Scholtissek. 1991. Evolution of pig influenza viruses. Virol. 183: 61-73 https://doi.org/10.1016/0042-6822(91)90118-U
  23. Song, D. S., J. Y. Lee, J. S. Oh, K. S. Lyoo, K. J. Yoon, Y. H. Park, and B. K. Park. 2003. Isolation of H3N2 swine influenza virus in South Korea. J. Vet. Diagn. Invest. 15: 30- 34 https://doi.org/10.1177/104063870301500107
  24. Sugimura, T., H. Yonemochi, T. Ogawa, K. Tanaka, and T. Kumagai. 1983. Isolation of a recombinant influenza virus (Hsw1N2) from swine in Japan. Arch. Virol. 66: 217-274
  25. Suzuki, H., M. Ito, T. Kimura, Y. Iwamori, and M. Iwamori. 2000. Inhibitory activity of sulphoglycolipid derivatives towards pancreatic trypsin. Glycoconj. J. 17: 787-793 https://doi.org/10.1023/A:1010988709764
  26. VanReeth, K., I. H. Brown, and M. Pensaert. 2000. Isolations of H1N2 influenza A virus from pigs in Belgium. Vet. Rec. 146: 588-589 https://doi.org/10.1136/vr.146.20.588
  27. Webby, R. J., S. L. Swenson, S. L. Krauss, P. J. Gerrish, S. M. Goyal, and R. G. Webster. 2000. Evolution of swine H3N2 influenza viruses in the United States. J. Virol. 74: 8243-8251 https://doi.org/10.1128/JVI.74.18.8243-8251.2000