Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effect of Estrus Induction in Shih-tzu Bitches by Administration of PMSG and HCG

PMSG와 hCG 병용 투여에 의한 Shih-tzu 견에서의 인공 발정 유기

  • Kim, B.S. (College of Veterinary Medicine, Chonnam University) ;
  • Son, C.H. (College of Veterinary Medicine, Chonnam University)
  • Published : 2007.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to induce the estrus in 9 anestrus Shih-tzu bitches by intramuscular injection of pregnant mare serum gonadotrophin (PMSG) 50IU/kg for 10 consecutive days and by intravenous injection of human chorionic gonadotrophin (hCG) 1,000IU/Head on Day 10. The day when the first injection of PMSG was counted as Day 0 of experiment. All of the bitches were monitored by vaginal discharges, displays the perineal region, vaginal swelling and male acceptances. The 9 bitches (100%) showed vaginal discharges and vaginal swelling, and were mated. The 5 bitches out of 9 bitches were pregnant (55.6%) and 4 bitches were non-pregnant (44.4%). The 3 bitches out of 5 pregnant bitches were spontaneously delivered (33.3%) and litter size were $1.66{\pm}1.15\;(1{\sim}3\;pups)$ pups. The 2 bitches were diagnosed as early embryonic death on days 38 and 41 after first injection of PMSG. These results indicated that rates of estrus induction, pregnancy and delivery were 100%, 55.6% and 33.3%, respectively, using PMSG and hCG.

무발정기에 속한 Shih-tzu 견 9두를 대상으로 하여 PMSG(50IU/kg)를 10일 동안 매일 근육주사 후 마지막 10일째에 hCG(1,000IU/head)를 정맥 주사하여 인공적으로 발정을 유기 하였다. 실험견 9두 중 9두(100%)에서 발정 출혈, 회음부 반사, 외음부 종대 및 수컷 허용 등의 임상적인 발정 증상이 관찰되었으며 그중 5두(55.6%)가 임신하였고 4두는 임신되지 않았다(44.4%). 임신견 5두 중 3두(33.3%)가 자연분만하였는데 분만시 산자수는 $1.66{\pm}1.15$두 $(1{\sim}3)$이었고, 나머지 2두(40.2%) 는 PMSG 첫 투여후 38일과 41일에 조기태아사 하였다. 따라서 Shih-tzu 견에서 PMSG와 hCG 병용 투여에 의한 인공 발정 유기 효과는 발정 발현율 100%, 임신률 55.6%, 분만율이 33.3%이었다.

Keywords

References

  1. Archbald LF, Baker BA, Clooney LL and Godke RA. 1980. A surgical method for collecting canine embryos after induction of estrus and ovulation with exogenous gonadotropins. Vet. Med. Small Animal Clinician, 75:230-238
  2. Bouchard GF, Gross S, Ganjam VK, Youngquist RS, Concannon PW and Krause GF. 1993. Oestrus induction in the bitch with the synthetic oestrogen diethylstilboestrol. J. Reporod. Fertil., 47:515-516
  3. Cain JL, Cain GR, Feldmen EC, Lasley BL and Stabenfelt GH. 1988. Use of pulsatile intravenous administration of gonadotrophin-releasing hormone to induce fertile estrus in bitches. Am. J. Vet. Res., 49:1993-1996
  4. Chaffaux S, Locci D, Pontois M, Deletang F and Thibier M. 1984. Induction of ovarian activity in anoestrous beagle bitches. Br. Vet. J., 140:191-195 https://doi.org/10.1016/0007-1935(84)90080-0
  5. Christiansen IJ. 1984. Reproduction in the dog and cat. 1 st ed., Bailliere Tindall, London, pp. 41-54
  6. Christie DW, Bailey JB and Bell ET. 1971. Classification of cell types in vaginal smears during the canine oestrous cycle. Br. Vet. J., 128:301-309
  7. Concannon PW and Lein DH. 1989. Current veterinary therapy, 10th ed., Saunders, Philadelphia, pp. 1269-1282
  8. Concannon PW, Lasley B and Vanderlip S. 1997. LH release induction of oestrus and fertile ovulations in response to pulsatile administration of GnRH to anoestruous dogs. J. Reprod. Fertil., 51:1-54
  9. Concannon PW. 1993. Biology of gonadotropin secretion in adult and prepubertal female dogs. J. Reprod. Fertil., 47: 3-27
  10. Feldman EC and Nelson RW. 2004. Canine and feline endocrinology and reproduction. 3rd ed., Saunders, Philadelphia, pp. 752-774
  11. Guyant L. 1988. Canine vaginal cytology, Veterinary Technician, 9:513-520
  12. Haaften BV, Dieleman SJ, Okkens AC and Willemse AH. 1989. Timing the mating of dogs on the basis of blood progesterone concentration. Vet. Rec., 125:524-526 https://doi.org/10.1136/vr.125.21.524
  13. Inaba T, Tani H, Gonda M, Nakagawa A, Phmura M and Mori J. 1998. Induction of fertile estrus in bitches using a sustained-release formulation of a GnRH agonist (leuprolide acetate). Theriogenology, 49:975-982 https://doi.org/10.1016/S0093-691X(98)00046-6
  14. Johnson CA. 2003. Small animal internal medicine. 3rd ed., Mosby, Philadelphia, pp. 847-869
  15. Kutzler MA. 2005. Induction and synchronization of estrus in dogs. Theriogenology, 64: 766-775 https://doi.org/10.1016/j.theriogenology.2005.05.025
  16. Post K. 1985. Canine vaginal cytology during the estrous cycle. Can. Vet. J., 26:101-104
  17. Rota A, Mollo A, Marinelli L, Gabai G and Vincenti L. 2003. Evaluation of cabergoline and buserelin efficacy for oestrous induction in the bitch. Reprod. Dom. Anim., 38:440-443 https://doi.org/10.1046/j.0936-6768.2003.00460.x
  18. Schutte AP. 1967. Canine vaginal cytology. II. Cyclic changes. J. Small Anim. Pract., 8: 307-311 https://doi.org/10.1111/j.1748-5827.1967.tb04555.x
  19. Shille VM. 1989. Textbook of veterinary internal medicine, Vol II. Saunders, Philadelphia, pp. 1777-1791
  20. Vanderlip SL, Wing AE, Felt P, Linkie D, Rivier J, Concannon PW and Lasley BL. 1987. Ovulation induction in anestrous bitches by pulsatile administration of gonadotropin-releasing hormone. Lab. Anim. Sci., 37:459-464
  21. Verstegen JP, Onclin K, Silva LD and Concannon PW. 1997. Termination of fobligate anestrus and induction of fertile ovarian cycles by administration of purified pig LH. J. Reprod. Fertil., 111 :35-40 https://doi.org/10.1530/jrf.0.1110035
  22. Verstegen JP, Onclin K, Silva LD and Concannon PW. 1999. Effect of stage of anestrus on the induction of estrus by the dopamine agonist cabergoline in dogs. Theriogenology, 51: 597-611 https://doi.org/10.1016/S0093-691X(99)00013-8
  23. Wanke M, Farina J, Loza M, Rebuelto M and Concanon PW. 1997. Induction of oestrus in bitches with normal and persistent anestrus using human menopausal gonadotropin (hMG). Theriogenology, 47:935-942 https://doi.org/10.1016/S0093-691X(97)00049-6
  24. Weilenmann R, Arnold S, Dobeli M, Rusch P and Zerobin K. 1993. Estrus induction in bitches by the administration of PMSG and HCG. Schweiz. Arch. Tierheilkd., 135:236-241
  25. Wright PJ. 1980. The induction of oestrus and ovulation in the bitch using pregnant mare serum gonadotrophin and human chorionic gonadotrophin. Aust. Vet. J., 56:137-140 https://doi.org/10.1111/j.1751-0813.1980.tb05650.x
  26. Zoldag L, Fekete S, Csaky I and Bersenyi A. 2001. Fertile estrus induced in bitches by bromocryptine, a dopamine agonist: a clinical trial. Theriogenology, 55:1657-1666 https://doi.org/10.1016/S0093-691X(01)00510-6
  27. 강병규, 최한선, 나진수, 손창호, 오기석, 이차범. 1988. 인공적 발정 유기견의 질세포상에 관한 연구. 대한수의학회지, 28:203-211
  28. 김방실, 고진성, 이순애, 조양택, 김재풍, 오기석, 김종택, 박인철, 김영홍, 손창호. 2005. Shih-tzu 견에서 혈장 progesterone과 Estradiol 17 $\beta$ 농도 측정에 의한 배란시기 및 교배 적기의 추정. 한국임상수의학회지, 22:253-258
  29. 김방실, 오기석, 김재풍, 배춘식, 김성호, 김종택, 박인철, 박상국, 손창호. 2006. Shih-tzu 견에서 발정 주기 동안 질세포 검사및 번식 호르몬측정에 의한 교배 적기 및 배란시기의 판정. 한국수정란이식학회지, 21:207-216
  30. 김방실, 이순애, 고진성, 황순선, 박철호, 오기석, 김종택, 박인철, 김영홍, 손창호. 2004. Shih-tzu 견에서 초음파검사에 의한 분만일 예시표의 확립. 대한수의학회지, 44:303-310
  31. 김정훈, 정경아, 강현구, 오기석, 박인철, 박상국, 한호재, 손창호. 2000. 진돗개에서 발정 주기 동안 질세포상과 번식호르몬의 관계. 한국임상수의학회지, 17:225-233
  32. 이주환, 김나리, 박인철, 오기석, 김세라, 박상국, 문진산, 배춘식, 김성호, 김영홍, 손창호. 2002. PMSG와 hCG 병용투여에 의해 인공 발정 유기된 진돗개에서 질 상피 세포 변화상. 한국임상수의학회지, 19:418-425