Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of Prostaglandins on In Vitro Development of Bovine Embryos

소 체외 수정란의 체외 발육에 미치는 Prostaglandins의 영향

  • Shin, S.O. (College of Animal Life Science, Kangwon National University) ;
  • Park, S.B. (National Livestock Research Institute, R.D.A) ;
  • Park, C.K. (College of Animal Life Science, Kangwon National University)
  • Published : 2007.03.31
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Abstract

This study was conducted to examine the effects of prostaglandin $F_2{\alpha}(PGF_2{\alpha})$ and prostaglandin $E_2 (PGE_2)$ on the expansion and hatching of bovine embryos. During the in vitro culture, embryos were cultured with the following groups: (1) 0, 1, 10 and 100ng/ml $PGF_2{\alpha}$ (2) 0, 1, 10 and 100 ng/ml $PGF_2{\alpha}$, (3) low concentration of $PGF_2{\alpha}$ ; low concentration of $PGF_2{\alpha}$, (1ng/ml : 1ng/ml), (4) low concentration of $PGF_2{\alpha}$ : high concentration of $PGF_2{\alpha}$ (1ng/ml : 10ng/ml) (5) high concentration of $PGF_2{\alpha}$ : low concentration of $PGE_2$ (10ng/ml 1ng/ml) (6) high concentration of $PGF_2{\alpha}$ : high concentration of $PGE_2$(10 ng/ml : 10ng/ml). In the results of this study, treatment of $PGF_2{\alpha}$ or $PGE_2$ did not affect in vitro development to blastocysts. However, the hatching rates of embryos cultured with 10ng/ml $PGE_2$(10.3%) and 1ng/ml $PGF_2{\alpha}$ 10ng/ml $PGE_2$(22.2%) were significantly (P<0.05) higher than in control (4.3% and 12.7%) and other treatment groups. All groups treated with high concentrations of $PGF_2{\alpha}$ showed decreased hatching rates. Thus, this results suggested that $PGF_2{\alpha}\;and\;PGE_2$ were concerned with the hatching in bovine embryos, and their effects on hatching were different by the concentrations.

본 연구는 소 체외 수정란의 팽창 및 hatching시 prostaglandin $F_2{\alpha}(PGF_2{\alpha})$와 prostaglandin $E_2(PGF_2)$의 영향에 대하여 검토하였다. 체외 수정란의 체외 배양시 다음과 같이 (1) 0, 1, 10 및 100ng/ml $PGF_2{\alpha}$, (2) 0, 1, 10 및 100ng/ml $PGE_2$, (3) low $PGF_2{\alpha}$(1ng/ml : 1ng/ml), (4) low $PGF_2{\alpha}\;:\;high\;PGE_2(1ng/ml\;:\;10ng/ml)$, (5) high $PGF_2{\alpha}\;:\;low\;PGE_2$ (10ng/ml : 1ng/ml), (6) high $PGF_2{\alpha}$ : high $PGE_2$(10ng/ml : 10ng/ml)로 나누어 처리하였다. 그 결과, $PGF_2{\alpha}$ 및 $PGE_2$의 단독 처리가 소 체외 수정란의 배반포로의 발달에는 영향을 미치지는 않았다. 그러나 10ng/ml $PGE_2$(10.3%) 그리고 1ng/ml $PGF_2{\alpha}$ : 10ng/ml $PGE_2$(22.2%)를 처리한 수정란의 hatching율에 있어서는 대조군(4.3% and 12.7%)이나 다른 처리구에 비하여 유의적으로 높은 결과를 나타냈다(P<0.05). 한편 높은 농도의 $PGF_2{\alpha}$를 처리한 수정란의 경우 hatching율이 감소하였다. 따라서 본 연구의 결과는 $PGF_2{\alpha}$ 및 $PGE_2$의 첨가가 소 수정란의 hatching율과 관련이 있으며, 이것은 농도에 따라 서로 다른 영향을 미칠 수 있을 것으로 추측되었다.

Keywords

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