Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Cyclofructans from Inulin by Saccharomyces cerevisiae Strain Displaying Cell-Surface Cycloinulooligosaccharide Fructanotransferase

  • Kim, Hyun-Jin (Department of Biomaterial Control(BK21 Program), Dong-Eui University) ;
  • Lee, Jae-Hyung (Department of Biomaterial Control(BK21 Program), Dong-Eui University) ;
  • Kim, Hyun-Chul (Bioneer Corp.) ;
  • Lee, Jin-Woo (Department of Biotechnology, Dong-A University) ;
  • Kim, Yeon-Hee (Department of Biotechnology, Osaka University) ;
  • Nam, Soo-Wan (Department of Biomaterial Control(BK21 Program), Dong-Eui University)
  • Published : 2007.04.30
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Abstract

The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans (GenBank access code AF222787) was expressed on the cell surface of Saccharomyces cerevisiae by fusing with Aga2p linked to the membrane-anchored protein Aga1p. The surface display of CFTase was confirmed by immunofluorescence microscopy and enzymatic assay. The optimized reaction conditions of surface-displayed CFTase were as follows; pH, 8.0; temperature, $50^{\circ}C$; enzyme amount, 30 milliunit; substrate concentration, 5%; inulin source, Jerusalem artichoke. As a result of the reaction with inulin, cycloinulohexaose was produced as a major product along with cycloinuloheptaose and cycloinulooctaose as minor products.

Keywords

References

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