Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Detection of Methicillin Resistance in Staphylococcus aureus Isolates Using Two-Step Triplex PCR and Conventional Methods

  • Cho, Joon-Il (Department of Food Science and Technology, Chung-Ang University) ;
  • Jung, Hye-Jin (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Young-Joon (Department of Food Science and Technology, Chung-Ang University) ;
  • Park, Sung-Hee (Department of Food Science and Technology, Chung-Ang University) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
  • Published : 2007.04.30
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Abstract

A two-step triplex PCR assay targeting the mecA, femA, and nuc genes was developed for the detection of methicillin resistance genes harbored by some Staphylococcus aureus isolates and for the simultaneous identification of such isolates at the species level. The triplex PCR revealed the presence of the femA and nuc genes in all the S. aureus isolates examined (n=105). Forty-four clinical isolates were mecA positive and no foodborne isolates were mecA positive. The PCR results had a 98 or 99% correlation with the results of PBP2a latex agglutination tests or oxacillin susceptibility tests, respectively.

Keywords

References

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