Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Metagenomic Analysis of BTEX-Contaminated Forest Soil Microcosm

  • Ji, Sang-Chun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Doc-Kyu (Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University) ;
  • Yoon, Jung-Hoon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Choong-Hwan (Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2007.04.30
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Abstract

A microcosmal experiment using a metagenomic technique was designed to assess the effect of BTEX (benzene, toluene, ethylbenzene, and xylenes) on an indigenous bacterial community in a Daejeon forest soil. A compositional shift of bacterial groups in an artificial BTEX-contaminated soil was examined by the 16S rDNA PCR-DGGE method. Phylogenetic analysis of 16S rDNAs in the dominant DGGE bands showed that the number of Actinobacteria and Bacillus populations increased. To confirm these observations, we performed PCR to amplify the 23S rDNA and 16S rDNA against the sample metagenome using Actinobacteria-targeting and Bacilli-specific primer sets, respectively. The result further confirmed that a bacterial community containing Actinobacteria and Bacillus was affected by BTEX.

Keywords

References

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