Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Ionic Liquid on the Kinetics of Peroxidase Catalysis

  • Lee, Yoon-Mi (Department of Chemical Engineering and Bioengineering, University of Ulsan) ;
  • Kwon, O-Yul (Department of Environmental Engineering, Seoul National University of Technology) ;
  • Yoo, Ik-Keun (Department of Chemical Engineering and Bioengineering, University of Ulsan) ;
  • Ryu, Keun-Garp (Department of Chemical Engineering and Bioengineering, University of Ulsan)
  • Published : 2007.04.30
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Abstract

The effect of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate $([BMIM][BF_4])$, on the horseradish peroxidase (HRP)-catalyzed oxidation of 2-methoxyphenol (guaiacol) with hydrogen peroxide $(H_2O_2)$ was investigated. HRP maintains its high activity in the aqueous mixtures containing various concentrations of the ionic liquid and even in 90% (v/v) ionic liquid. In order to minimize the effect of solution viscosity on the kinetic constants of HRP catalysis, the enzymatic reactions in the subsequent kinetic study were performed in water-ionic liquid mixtures containing 25% (v/v) ionic liquid at maximum. As the concentration of $[BMIM][BF_4]$ increased for the oxidation of guaiacol by HRP, the $K_m$ value increased with a slight decrease in the $K_{cat}$ value: The $K_m$ value increased from 2.8 mM in 100% (v/v) water to 22.5mM in 25% (v/v) ionic liquid, indicating that ionic liquid significantly weakens the binding affinity of guaiacol to HRP.

Keywords

References

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