Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of a Myostatin-like Gene from the Scallop Patinopecten yessoensis

  • Kim, Hyun-Woo (Department of Marine Biology, Pukyong National University) ;
  • Kim, Hak-Jun (Korea Polar Research Institute, Songdo Techno Park) ;
  • Yoo, Myong-Suk (Department of Marine Biology, Pukyong National University)
  • Published : 2007.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Myostatin (GDF8) is a growth factor that limits muscle tissue growth and development in vertebrates. We isolated a myostatin-like gene (Py-MSTN) from the marine invertebrate, the scallop Patinopecten yessoensis. Py-MSTN was highly expressed in the adductor muscle and in the gill unexpectedly. Amino acid analysis showed that Py-MSTN has 49% amino acid sequence identity and 64% similarity to human myostatin (Hs-MSTN), and 42% identity and 61% similarity to myoglianin, the only invertebrate homolog. These results indicated that Py-MSTN may be functionally similar to the vertebrate MSTN than the invertebrate homolog. Phylogenetic analysis suggested that Py-MSTN is an ancestral form of vertebrate MSTN and GDF11 and does not belong to other $TGF-{\beta}$ family members. Molecular modeling showed that Py-MSTN exhibits a similar tertiary structure to mammalian BMP7, a member of $TGF-{\beta}$ family. In addition, the amino acid residues which contact extracellular domain of the receptor were relavively conserved. Given these results, we propose that Py-MSTN is a functionally active member of the $TGF-{\beta}$ family and is involved In muscle growth and regulation.

Keywords

References

  1. Acosta, J., Y. Carpio, I. Borroto, O. Gonzalez and M.P. Estrada. 2005. Myostatin gene silenced by RNAi show a zebrafish giant phenotype. J. Biotechnol., 119, 324-331 https://doi.org/10.1016/j.jbiotec.2005.04.023
  2. Allendorph, G.P., W.W. Vale and S. Choe. 2006. Structure of the ternary signaling complex of a TGF-beta superfamily member. Proc. Natl. Acad. Sci. USA, 103, 7643-7648
  3. Amali, A.A., C.J. Lin, Y.H. Chen, W.L. Wang, H.Y. Gong, C.Y. Lee, Y.L. Ko, J.K. Lu, G.M. Her, T.T. Chen and J.L. Wu. 2004. Up-regulation of muscle-specific transcription factors during embryonic somitogenesis of zebrafish (Danio rerio) by knock-down of myostatin - I. Dev. Dyn., 229, 847-856 https://doi.org/10.1002/dvdy.10454
  4. Biga, P.R., S.B. Roberts, D.B. Iielv, L.A. McCauley, J.S. Moon, P. Collodi and F.W. Goetz. 2005. The isolation, characterization, and expression of a novel GDF11 gene and a second myostatin form in zebrafish, Danio rerio. Comp. Biochem. Physiol. B., 141, 218-230 https://doi.org/10.1016/j.cbpc.2005.03.004
  5. Griffith, D.L., P.C. Keck, T.K. Sampath, D.C. Rueger and W.D. Carlson. 1996. Three-dimensional structure of recombinant human osteogenic protein. 1: structural paradigm for the transforming growth factor beta superfamily. Proc. Natl. Acad. Sci. USA, 93, 878-883
  6. Jespersen, J., M. Kjaerand P. Schjerling. 2006. The possible role of myostatin in skeletal muscle atrophy and cachexia. Scand. J. Med. Sci. Sports, 16, 74-82 https://doi.org/10.1111/j.1600-0838.2005.00498.x
  7. Kim, H.W., E.S. Chang and D.L. Mykles. 2005a. Three calpains and ecdysone receptor in the land crab Gecarcinus lateralis: sequences, expression and effects of elevated ecdysteroid induced by eyestalk ablation. J. Exp. BioI., 208(Pt 16), 3177-3197 https://doi.org/10.1242/jeb.01754
  8. Kim, H.W., S.G. Lee and D.L. Mykles. 2005b. Ecdysteroid-responsive genes, RXR and E75, in the tropical land crab, Gecarcinus lateralis: differential tissue expression of multiple RXR isoforms generated at three alternative splicing sites in the hinge and ligand-binding domains. Mol. Cell. Endocrinol., 242, 80-95 https://doi.org/10.1016/j.mce.2005.08.001
  9. Ko, C.F, T.T. Chiou, T.T. Chen, J.L. Wu, J.C. Chen and J.K. Lu. 2007. Molecular cloning of myostatin gene and characterization of tissue-specific and developmental stage-specific expression of the gene in orange spotted grouper, Epinephelus coioides. Mar. Biotechnol., 9, 21-32
  10. Kocabas, A.M., H. Kucuktas, R.A. Dunham and Z. Liu. 2002. Molecular characterization and differential expression of the myostatin gene in channel catfish (Itaclurus punctatus). Biochim. Biophys. Acta, 1575, 99-107 https://doi.org/10.1016/S0167-4781(02)00289-0
  11. Lee, K.J., H.W. Kim, A.M. Gomez, E.S. Chang, J.A. Covi and D.L. Mykles. 2007. Molt-inhibiting hormone from the tropical land crab, Gecarcinus lateralis: Cloning, tissue expression, and expression of biologically active recombinant peptide in yeast. Gen. Comp. Endocrinol. 150, 505-513 https://doi.org/10.1016/j.ygcen.2006.09.013
  12. Lee, S.J. and A.C. McPherron. 2001. Regulation of myostatin activity and muscle growth. Proc. Natl. Acad Sci. USA, 98, 9306-9311
  13. Lo, P.C. and M. Frasch. 1999. Sequence and expression of myoglianin, a novel Drosophila gene of the TGF-beta superfamily. Mech Dev., 86, 171-175 https://doi.org/10.1016/S0925-4773(99)00108-2
  14. McPherron, A.C., A.M. Lawlerand S.J. Lee. 1997. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature., 387, 83-90 https://doi.org/10.1038/387083a0
  15. McPherron, A.C., A.M. Lawler and J. Lee. 1999. Regulation of anterior/posterior patterning of the axial skeIeton by growth/differentiation factor 11. Nat. Genet. 22, 260-264 https://doi.org/10.1038/10320
  16. McPherron, A.C. and S.J. Lee. 1997. Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. USA, 94, 12457-12461
  17. Nakashima, M., T. Toyono, A. Akamine and A. Joyner. 1999. Expression of growth/differentiation factor 11, a new member of the BMP/TGFbeta superfamily during mouse embryogenesis. Mech. Dev., 80, 185-189 https://doi.org/10.1016/S0925-4773(98)00205-6
  18. Nishi, M., A. Yasue, S. Nishimatu, T. Nohno, T. Yamaoka, M. ltakura, K. Moriyama, H. Ohuchi and S. Noji. 2002. A missense mutant myostatin causes hyperplasia without hypertrophy in the mouse muscle. Biochem. Biophys. Res. Comm., 293, 247-251 https://doi.org/10.1016/S0006-291X(02)00209-7
  19. Rescan, P.Y., I. Jutel and C. Ralliere. 2001. Two myostatin genes are differentially expressed in myotomal muscles of the trout (Oncorhynchus mykiss). J. Exp. BioI., 204, 3523-3529
  20. Stratil, A. and M. Kopecny. 1999. Genomic organization, sequence and polymorphism of the porcine myostatin (GDF8; MSTN) gene. Anim. Genet., 30, 468-470
  21. Thomas, M., B. Langley, C. Berry, M. Sharma, S. Kirk, J. Bass and R. Kambadur. 2000. Myostatin, a negative regulator of muscle growth, functions by inhibiting myoblast proliferation. J. Biol. Chem., 275, 40235-40243 https://doi.org/10.1074/jbc.M004356200
  22. Vianello, S., L. Brazzoduro, L. Dalla Valle, P. Belvedere and L. Colombo. 2003. Myostatin expression during development and chronic stress in zebrafish (Danio rerio). J. Endocrinol. 17, 6, 47-59
  23. Yang, J., T. Ratovitski, J.P. Brady, M.B. Solomon, K.D. Wells and R.J. Wall. 2001a. Expression of myostatin pro domain results in muscular transgenic mice. Mol. Reprod. Dev., 60, 351-361 https://doi.org/10.1002/mrd.1097
  24. Yang, W., H.X. Wang, Y. Chen, Y. Zhang and D.H. Zhu. 2001 b. Cloning, expression and purification of the chicken growth and differentiation factor - 8. Sheng Wu Gong Cheng Xue Bao, 17, 460-462