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어리연꽃 (Nymphoides indica (L.) O. Kuntze) 화경 배양으로부터 체세포배발생을 통한 식물체 재생

Plant Regeneration from Floral Stem Cultures of Nymphoides indica (L.) O. Kuntze. via Somatic Embryogenesis

  • 오명진 (한국생명공학연구원 생물자원센터) ;
  • 민성란 (식물유전체연구센터) ;
  • 유장렬 (식물유전체연구센터) ;
  • 김석원 (한국생명공학연구원 생물자원센터)
  • Oh, Myung-Jin (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Min, Sung-Ran (Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Liu, Jang-Ryol (Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Suk-Weon (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2007.03.31

초록

어리연꽃의 화경조직으로부터 형성된 캘러스로부터 체세포배발생을 통한 기내 식물체 재생체계를 확립하였다. 2,4-D가 첨가된 배양배지에 4주 배양 후 어리연꽃의 화경화경조직으로부터 연황색의 구형세포과가 발달하였으며 생장조절제가 첨가되지 않은 1/2MS 배지로 옮걱 명배양한 결과, 약 배양2주 후부터 녹색의 체세포배 유사구조의 발달이 관찰되었으며 정상적인 식물체로 발달하였다. 따라서 캘러스 발달 초기에 형성된 구형 세포괴 및 캘러스는 초기 단계의 체세포배이며 캘러스는 배발생캘러스임을 알 수 있었다. 화경조직으로부터 배발생캘러스 형성빈도는 0.1-0.3 mg/L 2,4-D가 첨가된 1/2MS 배지에서는 거의 100%이었으며 2,4-D 농도가 증가할수록 배발생캘러스의 형성빈도는 반대로 감소하였고 고농도 2,4-D 처리구에서는 캘러스 형성이 전혀 이루어지지 않았다. 한편 BA는 처리 농도에 상관없이 조직의 갈변 및 고사를 유발함으로써 체세포배 형성에 저해적임을 알 수 있었다. 어리연꽃의 잎조직의 경우는 화경조직에 비하여 연황색 구형 세포괴 형성 빈도가 매우 낮아 0.3 mg/L 2,4-D 처리구 (배발생캘러스 형성빈도 9.5%)를 제외하고는 나머지 모든 처리구에서 배발생캘러스의 형성을 관찰할 수 없었다. 현재 배발생캘러스의 현탁배양 체계를 확립중이며 본 연구에서 확립된 어리연꽃의 식물체 재생체계는 어리연꽃의 기내 대량증식 수단은 물론, 유용 형질 도입을 통한 분자육종의 수단으로 활용이 가능할 것으로 기대된다.

Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal medium, pale-yellow globular structures were developed into somatic embryos and normal plantlets. These results indicated that pale-yellow globular structures and calluses from floral stem were globular embryos and embryogenic calluses, respectively. The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2Ms medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). However, the higher concentration of 2,4-D resulted in decrease of the frequency of embryogenic callus formation. In this study, low concentration of 2,4-D had a stimulative role in embryogenic callus formation, whereas BA showed inhibitory role in callus formation. In comparison to floral stem, leaf explants showed low frequency of embryogenic callus formation. The highest frequency of embryogenic callus formation from leaf explants was 9.5% when leaf explants were cultured onto 1/2MS medium supplemented with 0.3 mg/L of 2,4-D. The plant regeneration system of Nymphoides indica established in this study, might be applied to mass proliferation, conservation of genetic resources and genetic transformation for molecular breeding.

키워드

참고문헌

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피인용 문헌

  1. High frequency somatic embryogenesis through leaf explant-derived callus culture in Muscari armeniacum cv. 'Early Giant' vol.39, pp.1, 2012, https://doi.org/10.5010/JPB.2012.39.1.069
  2. High frequency plant regeneration system for Nymphoides coreana via somatic embryogenesis from zygotic embryo-derived embryogenic cell suspension cultures vol.4, pp.2, 2010, https://doi.org/10.1007/s11816-010-0126-3