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The Korean Society of Phycology (한국조류학회(藻類))
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Immunomodulating Activity of a Fucoidan Isolated from Korean Undaria pinnatifida Sporophyll

  • Yoo, Yung-Choon (Dept. of Microbiology, Konyang University, Konyang University Hospital) ;
  • Kim, Woo-Jung (Dept. of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea) ;
  • Kim, So-Yeon (Dept. of Microbiology, Konyang University, Konyang University Hospital) ;
  • Kim, Sung-Min (Dept. of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea) ;
  • Chung, Mi-Kyung (Biomedicine Research Group, Hanrib Life Tech, Bio 21 Center) ;
  • Park, Joo-Woong (Biomedicine Research Group, Hanrib Life Tech, Bio 21 Center) ;
  • Suh, Hyun-Hyo (Dept. of Environmental Engineering, Jinju National University) ;
  • Lee, Kyung-Bok (Dept. of Microbiology, Konyang University, Konyang University Hospital) ;
  • Park, Yong-Il (Dept. of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea)
  • Published : 2007.12.01
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Abstract

A fucoidan, isolated from Korean Undaria pinnatifida spoprophyll (UP-F), was investigated for its immunomodulating activity on murine macrophages and splenocytes, and its activity was compared with that of fucoidan from Fucus vesiculosus (FV-F). Treatment of UP-F resulted in inhibition of the growth of murine macrophage RAW 264.7 cells, but its cytotoxicity was not observed in normal murine splenocytes. FV-F was shown to be highly cytotoxic to both immune cells, and its cytotoxic activity was higher than that of UP-F. Treatment of UP-F induced TNF-α in a dose-dependent manner from two types of macrophages, RAW 264.7 cells and murine peritoneal macrophages. The TNF-α-inducing activity of UP-F was higher than that of FV-F. UP-F also actively induced chemokines (RANTES and MIP-1α) from RAW 264.7 cells. Furthermore, treatment of UP-F gave rise to activation of murine splenocytes to produce cytokine (IL-6) and chemokines (RANTES and MIP-1α), showing significantly higher activity than that of FV-F. These results indicate that UP-F is less cytotoxic to immune cells than FV-F, and possesses immunomodulating activity to produce cytokines and chemokines from macrophages and splenocytes.

Keywords

References

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