한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)
- 제2권3호
- /
- Pages.192-197
- /
- 2007
- /
- 2383-5400(eISSN)
지질다당질로 자극한 넙치 백혈구의 발현유전자의 분석
Gene Expression Profiles in the Peripheral Blood Leukocytes (PBL) of Olive Flounder Paralichthys olivaceus after Stimulation with Lipoolysaccharide (LPS) in vitro
-
남보혜
(국립수산과학원 생명공학연구소) ;
- 문지영 (국립수산과학원 생명공학연구소) ;
-
김영옥
(국립수산과학원 생명공학연구소) ;
- 김우진 (국립수산과학원 생명공학연구소) ;
-
공희정
(국립수산과학원 생명공학연구소) ;
-
이상준
(국립수산과학원 생명공학연구소) ;
- 최태진 (국립수산과학원 생명공학연구소)
-
Nam, Bo-Hye
(Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
- Moon, Ji-Young (Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
-
Kim, Young-Ok
(Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
- Kim, Woo Jin (Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
-
Kong, Hee Jeong
(Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
-
Lee, Sang-Jun
(Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
- Choi, Tae-Jin (Biotechnology Research Institute, National Fisheries Research & Development Institute)
- 발행 : 2007.09.30
초록
넙치의 생체방어 및 면역관련 유전자의 대량확보를 위하여, 지질다당질로 자극한 넙치의 말초백혈구 cDNA library를 suppression subtractive hybridization 방법으로 제작하여 발현유전자의 발현 양상 및 특성을 분석하였다. 총 470개의 SSH 클론 중 기존에 밝혀진 유전자와 상동성을 보인 218개의 클론을 분석한 결과, 대표적인 전염증사이토카인 IL-1의 유인수용체인 IL-1RII가 34번의 가장 높은 빈도로 발현되었으며, IL-
Suppression subtractive hybridization (SSH) was performed to construct an cDNA library of olive flounder Paralichthys olivaceus peripheral blood leukocytes (PBL) stimulated with lipopolysaccharide (LPS). Total 470 clones were randomly selected and sequenced, 214 out of 470 sequences showed identities with known genes and 252 sequences were unknown genes. Among the 218 known genes, 34 sequences were found to be homologous to IL-1RII gene, and 14 sequences were identified as IL-
키워드