Toxicity of Binary Mixture of Cyanide and 3,5-dichlorophenol to Vibrio fischeri Determined by Newly Developed $N-tox^{(R)}$ Bioassay System

국내 개발된 $N-tox^{(R)}$ 생물검정 시스템을 이용한 시안과 3, 5-이염화페놀의 발광박테리아(Vibrio fischeri)에 대한 혼합 독성 영향 연구

  • Lee, Jung-Suk (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Lee, Kyu-Tae (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Kim, Chan-Kook (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Kim, Hye-Jin (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Lee, Chang-Hoon (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Lee, Jong-Hyeon (Institute of Environmental Protection and Safety, NeoEnBiz Co.)
  • 이정석 ((주)네오엔비즈 부설환경안전연구소) ;
  • 이규태 ((주)네오엔비즈 부설환경안전연구소) ;
  • 김찬국 ((주)네오엔비즈 부설환경안전연구소) ;
  • 김혜진 ((주)네오엔비즈 부설환경안전연구소) ;
  • 이창훈 ((주)네오엔비즈 부설환경안전연구소) ;
  • 이종현 ((주)네오엔비즈 부설환경 안전연구소)
  • Published : 2007.03.31

Abstract

Cyanides and dichlorophenols were important pollutants in industrial effluents of steel, petroleum, plastics, pesticides, synthetic dye and/or fiber manufacturing. The toxic effects of cyanide and 3, 5-dichlorophenol in the unary and binary solutions to Vibrio fischeri were determined using the newly developed $N-tox^{(R)}$ bioassay system. This bioassay system relies upon the attenuation of light intensity emitted by Vibrio fischeri exposed to various pollutants including metals and organic compounds. Most of studies dealing with toxicity of pollutants concerned single chemical species, while the organisms were typically exposed to pollutant mixtures. The present study showed that the toxicity of some binary combinations of cyanide and 3, 5-dichlorophenol significantly was lower than the predicted toxicity from the addicted model. This antagonistic interaction was well explained by chemical interaction model presented in this study.

Keywords

References

  1. 이규태. 마이크로톡스 생물검정법의 실험방법 개량과 현장 적용에 관한 연구, 서울대학교 대학원 박사학위논문 2002; 161
  2. Brix KV, Cardwell RD, Henderson DG and Marsden AR. Site-specific marine water-quality criterion for cyanide, Environ Toxicol Chem 2000; 19: 2323-2327 https://doi.org/10.1897/1551-5028(2000)019<2323:SSMWQC>2.3.CO;2
  3. Irwin RJ, VanMouwerik M, Stevens L, Seese MD and Basham W. Environmental Contaminants Encyclopedia. National Park Service, Water Resources Division, Fort Collins, Colorado, 1998
  4. International Standard Organization. 1998, Water quality-Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test), ISO standard 11348
  5. Kishino T and Kobayashi K. Relationship between toxicity and accumulation of chlorophenols at various pH, and their absorption mechanism in fish, Wat Res 1995; 29: 431-442 https://doi.org/10.1016/0043-1354(94)00189-E
  6. Kishino T and Kobayashi K. Studies on the mechanism of toxicity of chlorophenols found in fish through quantitative structure-activity relationships, Wat Res 1996; 30: 393-399 https://doi.org/10.1016/0043-1354(95)00152-2
  7. Escher BI and Schwarzenbach RP. Partitioning of substituted phenols in liposome-water, biomembrane-water, and octanol-water systems, Environ Sci Technol 1996; 30: 260-270 https://doi.org/10.1021/es9503084
  8. Lee YG, Hwang SH and Kim SD. Prediction the toxicity of substituted phenols to aquatic species and its changes in the stream and effluent waters, Arch Environ Contam Toxicol 2006; 50: 213-219 https://doi.org/10.1007/s00244-004-1259-6
  9. Hsieh CY, Tsai MH, Ryan DK and Pancorbo OC. Toxicity of the 13 priority pollutant metals to Vibrio fisheri in the $Microtox^{\circledR}$ chronic toxicity test, Sci Total Environ 2004; 320(1-5): 37-50 https://doi.org/10.1016/S0048-9697(03)00452-2
  10. Salizzato M, Pavoni B, Ghirardini AV and Ghetti PF. Sediment toxicity measured using Vibrio fischeri as related to the concentrations of organic (PCBs, PAHs) and inorganic (metals, sulphur) pollutants, Chemosphere 1998; 36(14): 2949-2968 https://doi.org/10.1016/S0045-6535(98)00001-0
  11. Onorati F and Mecozzi M. Effects of two diluents in the $Microtox^{\circledR}$ toxicity bioassay with marine sediments, Chemosphere 2004; 54(5): 679-687 https://doi.org/10.1016/j.chemosphere.2003.09.010
  12. Rider CV and LeBlanc GA. An Integrated Addition and Interaction Model for Assessing Toxicity of Chemical Mixtures, Toxicol Sci 2005; 87(2): 520-528 https://doi.org/10.1093/toxsci/kfi247
  13. USEPA. Ambient water quality criteria for Cyanide, U.S. Environmental Protection Agency Office of Research and Development. Duluth, Minnesota Narragansett, RI, USA, 1985
  14. Zwart DD and Slooff W. The Microtox as an alternative assay in the acute toxicity assessment of water pollutants, Aqua Toxicol 1983; 4(2): 129-138 https://doi.org/10.1016/0166-445X(83)90050-4