Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Influence of Electrochemical Oxidation Potential on Biofilm Structure and Bacterial Dissimilation in Wastewater Treatment Bioreactor

오수처리 반응기에서 생물막 매개체에 부과한 전기화학적 산화전위가 생물막의 구조와 미생물의 대사에 미치는 영향

  • Na, Byung-Kwan (Department of Biological Engineering, Seokyeong University) ;
  • Park, Doo-Hyun (Department of Biological Engineering, Seokyeong University)
  • 나병관 (서경대학교 생물공학과) ;
  • 박두현 (서경대학교 생물공학과)
  • Published : 2007.03.28
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Abstract

Biofilm media was equipped in two-compartmented wastewater treatment bioreactor which was separated by porcelain septum. DC 2.0 volt of electric potential was charged to anodic (oxidative) biofilm media (ABM) to induce oxidation potential but not to that of carbon (neutral) biofilm media (CBM) that was used for control test. Biofilm structure, biomass variation, Off variation and wastewater treatment efficiency in the bioreactor equipped with ABM (ABM-bioreactor) and CBM (CBM-bioreactor). Time-coursed variation of biofilm structure forming on surface of ABM and CBM was observed by scanning electron microscopy. The biofilm growing on ABM was dispersed on surface and was not completely covered the media but the biofilm growing on CBM was continuously increased and finally covered the media. The ORP of CBM was decreased to 100 mV, which was reciprocally proportional to the biomass growth. However, the ORP of ABM was about 800 mV, which was maintained during operation for about 60 days. The treatment efficiency of COD in the ABM bioreactor was 2 times higher than those in the CBM bioreactor. From these results, we proposed that electrochemical oxidation potential charged to biofilm media may inhibit formation of biofilm extremely condensed and activate bacterial cell metabolism.

생물막 매개체를 도자기 격막으로 구획된 오수처리 반응기에 장착하고 직류 2volt의 전압을 부과하여 생물막 매개체가 산화 전위를 유지할 수 있게 유도하였다. 반면 대조실험을 위해 사용한 반응조의 생물막 매개체에는 전압을 부과하지 않았다 ABM-반응기와 CBM-반응기에서 배양시간에 따른 생물막의 구조, 생물량의 변화, 오수처리 효율 등을 측정하여 상호 차이를 비교하였다. 전자현미경으로 관찰한 ABM의 생물막은 CBM의 생물막에 비해 분산성이 크고 미생물이 과밀하게 성장하지 않았으나 CBM의 생물막은 배양 시간에 비례하여 지속적으로 성장하면서 생물막 매개체를 완전히 덮어 과밀 생물막을 형성하였다. ABM의 ORP는 CBM의 ORP 100 mV에 비해 매우 큰 차이를 보이는 800 mV를 유지하였으며, 반응액의 ORP 또한 ABM과 CBM의 영향을 받아 각각 550 mV와 400 mV를 유지하였다. ABM-반응기에서 오수처리 효율은 CBM-반응기에서 오수처리 효율의 약 2배 정도의 차이를 나타내었다. 이러한 결과로부터 생물막 매개체에 부과한 양전위의 전기 에너지는 생물막을 구성하는 미생물 과밀현상을 억제하고 매개체의 ORP를 높게 유지함으로서 미생물의 대사 활성을 촉진하고 결과적으로 오수내 함유된 유기물의 산화효율을 증가시키는 작용이 있는 것으로 확인되었다.

Keywords

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