Increasing Production in Korean Shrimp Farms with White-Spot Syndrome Virus PCR-Negative Brood Stock

  • Seok, Seung-Hyeok (Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University) ;
  • Baek, Min-Won (Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University) ;
  • Lee, Hui-Young (Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University) ;
  • Kim, Dong-Jae (Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University) ;
  • Chun, Myung-Sun (Dong-a Science, Science Culture Research Center) ;
  • Kim, Jong-Sheek (National Fisheries Research and Development Institute) ;
  • Chang, Se-Ok (Shin Chon Feed Co., Ltd.) ;
  • Park, Jae-Hak (Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University)
  • Published : 2007.03.31

Abstract

White-spot syndrome virus (WSSV) is a devastating, infectious virus affecting shrimp. Although sensitive techniques involving PCR have been developed to assist farmers in screening shrimp (brood stock) for WSSV prior to stocking ponds, such practices have not yet been applied in Korea. Despite the rationality of implementing screening, there has been some doubt as to whether the stocking of WSSV-PCR-negative fly epidemiologically decreases white-spot disease outbreaks. Here, we report a retrospective analysis of data from shrimp farms in the western coast of Korea where WSSV-PCR-negative brood stocks were used to stock rearing ponds. A total of 366 shrimp from Heuksan Island were sampled for WSSV with PCR. Of the tested shrimp, 7.2% (28 brood stocks) were identified as WSSV positive; only WSSV-PCR-negative shrimp were used for brood stocks. Total unit production (final shrimp production/ the area of the ponds) was higher, at 1.96, in ponds where WSSV-PCR-negative shrimp were used, as compared with 1.02 in other ponds in Korea in 2004. This retrospective analysis of WSSV in Korea may be useful to the shrimp aquaculture industry, suggesting a testable hypothesis that may contribute to the eventual control of WSSV outbreaks.

Keywords

References

  1. Cen, F. 1998. The existing condition and development strategy of shrimp culture industry in China, pp. 32-38. In Y. Q. Su. (ed.). The Health Culture of Shrimps. China Ocean Press, Beijing
  2. Chang, P., C. Lo, Y. Wang, and G. H. Kou. 1996. Identification of white spot syndrome associated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situ hybridization. Dis. Aquat. Org. 27: 131-139 https://doi.org/10.3354/dao027131
  3. Chen, S. N. 1995. Current status of shrimp aquaculture in Taiwan, pp. 29-23. In Browdy, C. L. and J. S. Hopkins (eds.), Swimming Through Troubled Water. Proceedings of the Special Session on Shrimp Farming. Aquaculture '95. World Aquaculture Society, Baton Rouge, LA, U.S.A
  4. Corsin, F., T. T. Phi, L. H. Phuoc, N. T. N. Tinh, N. V. Hao, C. V. Mohan, J. F. Turnbull, and K. L. Morgan. 2002. Problems and solutions with the design and execution of an epidemiological study of white spot disease in black tiger shrimp (Penaeus monodon) in Vietnam. Prev. Vet. Med. 53: 117-132 https://doi.org/10.1016/S0167-5877(01)00265-3
  5. Flegel, T. W. 1997. Special topic review: Major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World J. Microbiol. Biotechnol. 13: 433-442 https://doi.org/10.1023/A:1018580301578
  6. Hossain, M. S., A. Chakraborty, B. Joseph, S. K. Otta, I. Karunasagar, and I. Karunasagar. 2001. Detection of new hosts for white spot syndrome virus of shrimp using nested polymerase chain reaction. Aquaculture 198: 1-11 https://doi.org/10.1016/S0044-8486(00)00571-8
  7. Kanchanaphum, P., C. Wongteerasupaya, N. Sitidilokratana, Y. Boonsaeng, S. Panyim, A. Tassanakajon, B. Withyachumnarnkul, and T. W. Flegel. 1998. Experimental transmission of white spot syndrome virus (WSSV) from crabs to shrimp Penaeus monodon. Dis. Aquat. Organ. 11: 1-7 https://doi.org/10.3354/dao011001
  8. Khadijah, S., S. Y. Neo, M. S. Hossain, L. D. Miller, S. Mathavan, and J. Kwang. 2003. Identification of white spot syndrome virus latency-related genes in specific-pathogenfree shrimps by use of a microarray. J. Virol. 77: 10162- 10167 https://doi.org/10.1128/JVI.77.18.10162-10167.2003
  9. Kim, C. K., P. K. Kim, S. G. Sohn, D. S. Sim, M. A. Park, M. S. Heo, T. H. Lee, and J. D. Lee. 1998. Development of a polymerase chain reaction (PCR) procedure for the detection of baculovirus associated with white spot syndrome (WSSV) in penaeid shrimp. J. Fish Dis. 21: 11-17 https://doi.org/10.1046/j.1365-2761.1998.00324.x
  10. Lan, J., P. Pratanpipat, G. Nash, S. Wongwisansri, B. Wongteerasupaya, B. Withyachumnarnkul, S. Thammasart, and C. Lohawattanakul. 1996. Carrier and susceptible host of the systemic ectodermal and mesodermal baculovirus, the causative agent of white-spot disease in penaeid shrimp, pp 213-214. In: World Aquaculture '96 Book of Abstracts, The 1996 Annual Meeting of the World Aquaculture Society, January 29-February 2 1996, Queen Sirikit National Convention Center, Bangkok
  11. Lee, W. W., B. J. Lee, Y. Lee, Y. S. Lee, and J. H. Park. 2000. In situ hybridization of white spot disease virus in experimentally infected Penaeid shrimp. J. Microbiol. Biotechnol. 10: 215-220
  12. Lightner, D. V. 1996. Viral diseases, pp. 1-72. In McVey, A. (ed.), A Handbook of Shrimp Pathology and Diagnostic Procedures for Disease of Cultured Penaeid Shrimp. World Aquaculture Society, Baton Rouge, LA, U.S.A
  13. Lo, C. F., C. H. Ho, C. H. Chen, K. F. Liu, Y. L. Chiu, P. Y. Yeh, S. E. Peng, and H. C. Hsu. 1997. Detection and tissue tropism of white spot syndrome vaculovirus (WSSV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Dis. Aquat. Organ. 30: 53-72 https://doi.org/10.3354/dao030053
  14. Lo, C. F., C. H. Ho, C. H. Peng, H. C. Hsu, Y. L. Chiu, C. F. Chang, K. F. Liu, M. S. Su, C. H. Wang, and G. H. Kou. 1996. White spot syndrome (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Dis. Aquat. Organ. 27: 212-225
  15. Lo, C. F., J. H. Leu, C. H. Ho, C. H. Chen, S. E. Peng, Y. T. Chen, C. M. Chou, P. Y. Yeh, C. J. Huang, H. Y. Chou, C. H. Wang, and G. H. Kou. 1996. Detection of baculovirus associated with white spot syndrome (WSSV) in penaeid shrimps using polymerase chain reaction. Dis. Aquat. Org. 25: 133-141 https://doi.org/10.3354/dao025133
  16. Lotz, J. M. and M. A. Soto. 2002. Model of white spot syndrome virus (WSSV) epidemics in Litopenaeus vannamei. Dis. Aquat. Organ. 50: 199-209 https://doi.org/10.3354/dao050199
  17. Magbanua, F. O., K. T. Natividad, V. P. Migo, C. G. Alfafara, F. O. de la Pena, R. O. Miranda, J. D. Albaladejo, and E. C. Nadala. 2000. White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines. Dis. Aquat. Organ. 10: 77-82
  18. MOMAF (Ministry of Maritime Affairs and Fisheries of Korea). 2004. Fishery Production Survey. Samsung Elite Publish. Inc
  19. Otta, S. K., G. Shubha, B. Joseph, A. Chakraborty, I. Karunasagar, and I. Karunasagar. 1999. Polymerase chain reaction (PCR) detection of white spot syndrome virus (WSSV) in cultured and wild crustaceans in India. Dis. Aquat. Organ. 38: 67-70 https://doi.org/10.3354/dao038067
  20. Park, J. H., Y. S. Lee, S. Lee, and Y. Lee. 1998. An infectious viral disease of penaeid shrimp newly found in Korea. Dis. Aquat. Organ. 34: 71-75 https://doi.org/10.3354/dao034071
  21. Seok, S. H., J. H. Park, S. A. Cho, M. W. Baek, H. Y. Lee, D. J. Kim, and J. H. Park. 2004. Cloning and sequencing of envelop protein (VP19, VP28) and nucleocapsid proteins (VP15, VP35) of a white spot syndrome virus isolated from Korean shrimp. Dis. Aquat. Organ. 60: 85-88 https://doi.org/10.3354/dao060085
  22. Shin, E. J., J. H. Park, and Y. H. 2001. White spot syndrome virus in penaeid shrimp cultured in Korea. J. Microbiol. Biotechnol. 11: 394-398
  23. van Hulten, M. C., J. Witteveldt, S. Peters, N. Kloosterboer, R. Tarchini, M. Fiers, H. Sandbrink, R. K. Lankhorst, and J. M. Vlak. 2001. The white spot syndrome virus DNA genome sequence. Virology 286: 7-22 https://doi.org/10.1006/viro.2001.1002
  24. Vaseeharan, B., R. Jayakumar, and P. Ramasamy. 2003. PCR-based detection of white spot syndrome virus in cultured and captured crustaceans in India. Lett. Appl. Microbiol. 37: 443-447 https://doi.org/10.1046/j.1472-765X.2003.01428.x
  25. Wang, C. H., C. F. Lo, P. S. Chang, and G. H. Kou. 1998. Experimental infection of white spot baculovirus in some cultured and wild decapods in Taiwan. Aquaculture 164: 221-231 https://doi.org/10.1016/S0044-8486(98)00188-4
  26. Wongwisansri, S. 1996. PCR-based method and in situ hybridization method for detection of white-spot virus in penaeid shrimp. Master's Thesis, Department of Biochemistry, Faculty of Science, Mahidol University
  27. Yang, F., J. He, X. Lin, Q. Li, D. Pan, X. Zhanggg, and X. Xu. 2001. Complete genome sequence of the shrimp white spot bacilliform virus. J. Virol. 75: 11811-11820 https://doi.org/10.1128/JVI.75.23.11811-11820.2001
  28. Zhan, W. B. and Y. H. Wang. 1988. White spot syndrome virus infection of cultured shrimp in China. J. Aquat. Anim. Health 10: 405-410 https://doi.org/10.1577/1548-8667(1998)010<0405:WSSVIO>2.0.CO;2