사구식물 연관 세균 군집의 분자계통학적 연구

Molecular Systematic Study of Bacterial Community Associated with Sand Dune Plants

  • 도진옥 (대전대학교 산업과학대학 미생물생명공학과) ;
  • 박성주 (대전대학교 산업과학대학 미생물생명공학과) ;
  • 김승범 (충남대학교 생명과학부 미생물학)
  • Do, Jin-Ok (Department of Microbiology and Biotechnology, Daejeon University) ;
  • Park, Seong-Joo (Department of Microbiology and Biotechnology, Daejeon University) ;
  • Kim, Seung-Bum (Department of Microbiology, Chungnam National University)
  • 발행 : 2007.11.30

초록

충남 태안군 일대 4곳의 해안사구 지역에서 자생하는 9종의 사구식물 연관 근권세균 다양성을 2003년 10월부터 2004년 3월까지의 기간 동안 3차례에 걸쳐 denaturing gradient gel electrophoresis (DGGE) 기법을 이용하여 조사하였다. 그 결과 한 밴드가 계속 모든 시료에서 우점하는 것으로 나타났으며, DNA 염기서열 분석에 의하여 Lysobacter enzymogenes와 가장 유사한 것으로 나타났다. 기타 주요 밴드들은 Pseudomonas와 Bacillus 속의 균주들로 동정되었다. L. enzymogenes가 9종의 식물 모두에서 지역이나 조사시기에 관계없이 우점적으로 나타난 것은 이전의 클론 분석을 통한 결과와 일치한다 (Lee et al. 2006a). Bacillus에 속한 밴드들이 모든 조사에서 출현하였으며, Pseudomonas 속 밴드들은 2003년 12월 조사에서 두드러졌다. DGGE 분석만으로는 Lysobacter가 사구식물에 가지는 중요성을 파악할 수는 없으나 건강한 개체에 지속적으로 발견되는 것으로 보아 Lysobacter의 존재는 식물에 긍정적인 영향을 가지는 것으로 사료된다.

The rhizobacterial diversity associated with 9 native plant species inhabiting coastal sand dunes in Tae-an area, Chungnam Province, was studied using the denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis over three times from October 2003 to March 2004. One dominant band commonly occurred in all of the rhizosphere samples, which was identified as that of Lysobacter enzymogenes. The other common bands included those derived from species of Pseudomonas and Bacillus. It was notable that L. enzymogenes was dominant in all of the 9 plant species and such dominance was consistent throughout the whole sampling period, which confirms the previous study by Lee et al. (2006a). The Bacillus bands were detected in all of the three samplings, and those of Pseudomonas were notable in the samples of December 2003. By the DGGE analysis alone, the significance of Lysobacter to the sand dune plants is not clear. However, considering their presence in healthy plants and the dominance in all plant species, Lysobacter may have positive roles in the survival or growth of the plants in sand dune area.

키워드

참고문헌

  1. 고성덕, 박주영. 2003. 충남 보령군 신두리 사구의 arbuscular mycorrhizal fungi (AMF)의 종조성과 분포에 관한 연구. Bullet. Sci. Edu. 19:189-203
  2. 김준태, 리기현, 정병철, 김종균. 1993. 사구식물과 Arbuscular 내생 균근균의 공생 특성. Kor. J. Mycol. 21:235-245
  3. 변무섭, 박준모. 2002. 우이도 사구의 식물상 및 비오톱 보전에 관한 연구. 한국산림휴양학회지 6:93-101
  4. 안영희. 2003. 신두리 해안 사구지 식생의 식물사회학적 연구. J. Kor. Env. Res. Reveg. Tech. 6:29-40
  5. 이우철, 전상근. 1983. 한국 해안식물의 생태학적 연구-남해안의 사구식물 군락의 종조성과 현존량. Kor. J. Ecol. 6:177-186
  6. 이우철, 전상근. 1984. 한국 해안식물의 생태학적 연구-서해안의 사구식생에 관하여. Kor. J. Ecol. 7:74-84
  7. 정용규, 김종원. 1998. 한국의 해안사구 식생. Kor. J. Ecol. 21:257-262
  8. Bae HS, WT Im and ST Lee. 2005. Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int. J. Syst. Evol. Microbiol. 55:1155-1161 https://doi.org/10.1099/ijs.0.63399-0
  9. Christensen P and FD Cook. 1978. Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. Int. J. Syst. Bacteriol. 28:367-393 https://doi.org/10.1099/00207713-28-3-367
  10. Clough KS and JC Sutton. 1978. Direct observation of fungal aggregates in sand dune soil. Can. J. Microbiol. 24:333-335 https://doi.org/10.1139/m78-056
  11. Epstein DM and PC Wensink. 1988. The alpha-lytic protease gene of Lysobacter enzymogenes. The nucleo-tide sequence predicts a large prepro-peptide with homology to pro-peptides of other chymotrypsin-like enzymes. J. Biol. Chem. 263:16586-16590
  12. Hagler AN, CA Rosa, PB Morais, LC Mendonca-Hagler, GM Franco, FV Araujo and CA Soares. 1993. Yeasts and coliform bacteria of water accumulated in bromeli-ads of mangrove and sand dune ecosystems of southeast Brazil. Can. J. 39: 973-977 https://doi.org/10.1139/m93-146
  13. Huguet V, JM Batzli, JF Zimpfer, P Normand, JO Dawson and MP Fernandez. 2001. Diversity and specificity of Frankia strains in nodules of sympatric Myrica gale, Alnus incana and Shepherdia canadensis determined by rrs gene polymorphism. Appl. Environ. Microbiol. 67:2116-2122 https://doi.org/10.1128/AEM.67.5.2116-2122.2001
  14. Im BS, JS Lee and HS Kim. 2003. Changes of the coastal sand dune vegetation after the construction of embank-ment in Anmado. Kor. J. Ecol. 26:103-108 https://doi.org/10.5141/JEFB.2003.26.3.103
  15. Kowalchuk GA, JR Stephen, W De Boer, JI Prosser, TM Emb-ley and JW Woldendorp. 1997. Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteriain coastal sand dunes by dena-turing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments. Appl. Environ. Microbiol. 63:1489-1497
  16. Kowalchuk GA, FA deSouza and JA van Veen. 2002. Community analysis of arbuscular mycorrhizal fungi associated with Ammophila arenaria in Dutch coastal sand dune. Mol. Ecol. 11:571-581 https://doi.org/10.1046/j.0962-1083.2001.01457.x
  17. Lee MS, JO Do, MS Park, S Jung, KH Lee, KS Bae, SJ Park and SB Kim. 2006a. Dominance of Lysobacter sp. in the rhizosphere of two coastal sand dune plant species, Calystegia soldanella and Elymus mollis. Antonie Leeuwenhoek 90:19-27 https://doi.org/10.1007/s10482-006-9056-z
  18. Lee JW, WT Im, MK Kim and DC Yang. 2006b. Lysobacter koreensis sp. nov., isolated from a ginseng field. Int. J. Syst. Evol. Microbiol. 56:231-235 https://doi.org/10.1099/ijs.0.63955-0
  19. Maremmani A, S Bedini, I Matosevic, PE Tomei and M Giovannetti. 2003. Type of mycorrhizal associations in two coastal nature researves of the editerranean basin. Mycorrhiza 13:33-40 https://doi.org/10.1007/s00572-002-0194-5
  20. Matekwor Ahulu E, M Nakata and M Nonaka. 2005. Arumand Paris-type arbuscular mycorrhizas in a mixed pine forest on sand dune soil in Niigata Pre-fecture, central Honshu, Japan. Mycorrhiza 15:129-136 https://doi.org/10.1007/s00572-004-0310-9
  21. Morais PB, CA Rosa, AN Hagler and LC Mendonca-Hagler. 1994. Yeast communities of the cactus Pilo-socereus arrabidae as resources for larval and adult stages of Drosophila serido. Antonie Leeuwenhoek 66:313-317 https://doi.org/10.1007/BF00882766
  22. Mudryk ZJ. 2005. Occurrence and distribution antibiotic resistance of heterotrophic bacteria isolated from a marine beach. Mar. Pollut. Bull. 50:80-86 https://doi.org/10.1016/j.marpolbul.2004.10.001
  23. Park MS, SR Jung, KH Lee, MS Lee, JO Do, SB Kim and KS Bae. 2005. Isolation and characterization of bacterial associated with two sand dune plants species, Calystegia soldanella and Elymus mollis. J. Microbiol. 43:219-227
  24. Rosa CA, MA Lachance, WT Starmer, JS Barker, JM Bowles and B Schlag-Edler. 1999. Kodamaea nitidulidarum, Candida restingae and Kodamaea anthophila, three new related yeast species from ephemeral flowers. Int. J. Syst. Bacteriol. 49:309-318 https://doi.org/10.1099/00207713-49-1-309
  25. Shin DS, MS Park, SR Jung, MS Lee, KH Lee, KS Bae and SB Kim. 2007. Plant growth-promoting potential of endophytic bacteria isolated from roots of coastal sand dune plants. J. Microbiol. Biotechnol. 17:1361-1368
  26. Weon HY, BY Kim, YK Baek, SH Yoo, SW Kwon, E Stackebrandt and SJ Go. 2006. Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. Int. J. Syst. Evol. Microbiol. 56:947-951 https://doi.org/10.1099/ijs.0.64095-0