Establishment of artificial cultivation technique of Cordyceps militaris using a grain media

곡물배지를 이용한 번데기동충하초 재배기술

  • Yu, Young-Jin (Jeollabuk-do Agricultural Research and Bio-Resources) ;
  • Seo, Kyoung-Won (Jeollabuk-do Agricultural Research and Bio-Resources) ;
  • Lee, Kong-Joon (Jeollabuk-do Agricultural Research and Bio-Resources) ;
  • Choi, Dong-Chil (Jeollabuk-do Agricultural Research and Bio-Resources) ;
  • Choi, Joung-Sik (Jeollabuk-do Agricultural Research and Bio-Resources) ;
  • Park, Joung-Sik (National Institute of Agricultural Science and Technology) ;
  • Sung, Jae-Mo (Department of Environmental Biology, Kangwon National University)
  • Published : 2007.06.30

Abstract

This study was carried out to investigate the conditions of liquid culture and fruiting body formation of Cordyceps militaris using grain mediacontained the brown rice and black purple rice. Liquid mycelial growth and density of Cordyceps militaris were the highest in the medium of malt extract 0.7% and pH 5.0~5.5. The optimal condition of formation the fruiting bodies were shown to be temperature of $17^{\circ}C$, light intensity of 600 lux, $C0_2$ of 500ppm and humidity intensity of 95% produced highest amount of fruit bodies.

본 연구는 번데기동충하초를 곡물배지를 이용하여 자실체를 형성하는 실험으로 액체종균제조에 적합한 조건은 맥아추출배지를 농도 0.7%, pH 5~5.5,으로 조정하였을 때 균사의 건중량은 $3.9{\sim}4.1mg/m{\ell}$로 양호 하였다. 자실체 발생 최적 환경조건은 습도 95%, 온도 $17^{\circ}C$, 조도 600 lx, 이산화탄소농도 500ppm하였을 때, 곡물배지 일반현미와 흑미에서 24.3g/병, 20.5g/병의 자실체가 형성되었다.

Keywords