Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Microbiological characteristics of Vibrio harveyi

Vibrio harveyi의 미생물학적 특성

  • Won, Kyoung-Mi (Institute of Fisheries Science, Pukyong National University) ;
  • Choi, Jeong-Hyun (East Sea Mariculture Research Center, National Fishereis Research and Development Institute) ;
  • Kim, Yi-Cheong (East Sea Mariculture Research Center, National Fishereis Research and Development Institute) ;
  • Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
  • 원경미 (부경대학교 수산과학연구소) ;
  • 최정현 (국립수산과학원 동해특성화연구센터) ;
  • 김이청 (국립수산과학원 동해특성화연구센터) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Published : 20071200
Download PDF
⟨ Previous Next ⟩

Abstract

In 1999, Vibrio harveyi infection occurred among cultured marine finfishes including olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), and turbot (Scophtalmus maximus) in the province of Gyeongsang, Korea. We examined the various microbiological characteristics of this V. harveyi strains. V. harveyi was grown well in the 3% NaCl at 30℃. The swarming activity appeared to be an one of the characteristic properties of the V. harveyi was the highest in the 2% NaCl concentration at TSA medium. These cells were elongated and had the several lateral flagella which is not waved and shorter than polar flagella. It was not luminescent in the all isolated strains. Whole cell of the V. harveyi had a major protein of 50 kDa and presented various band around of 40 kDa in strains.

본 연구에서는 우리 나라에서 분리된 Vibrio harveyi의 미생물학적 특성을 조사하여 V. harveyi 연구의 기초적인 정보를 제공하고자 하였다. V. harveyi는 염분 농도 3%, 배양 온도 30℃에서 가장 잘 증식하였다. Swarming 운동은 1.5% NaCl 첨가 TSA에서 가장 활발하였으며, swarming 운동시 균체는 길어지며, 짧고 곧은 다수의 lateral flagella가 생성된 것을 확인하였다. 우리나라에서 분리된 V. harveyi는 luminometer로 직접 검출하는 방법과 luciferase gene을 검출하는 방법 모두에서 음성으로 나타나, 발광능이 없는 것으로 조사되었다. V. harveyi의 균체 단백질은 50 kDa 부근의 major protein을 가지며, 균주에 따라 40 kDa 근처의 상이한 band pattern을 보였다.

Keywords

References

  1. Abraham, T.J. and Manley, R.: Luminous and nonluminous vibiro harveyi associated with shell disease in cultured Penaeus indicus. J. Aqua. Trop., 10: 273-276, 1995
  2. Baumann, P. and Baumann, L.: Biology of the marine enterobacteria: genera Beneckea and Photobacterium. Annu. Rev. Microbiol., 31:39-61, 1977 https://doi.org/10.1146/annurev.mi.31.100177.000351
  3. Belas, M.R. and Colwell, R.R.: Scanning electron microscope observation of the swarming phenomenon of Vibrio parahaemolyticus. J. Bacteriol., 150:956-959. 1982
  4. Clark. G.: Staining procedures (4th ed.). Williams & Wilkins. Baltimore, USA, 1981
  5. Follett, E.A.C. and Gordon, J.: An electron microscope study of Vibrio flagella. J. Gen. Microbiol., 32: 235-239. 1968
  6. Fuqua, C., Winans. S.C. and Greenberg, E.P.: Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators. Annu. Rev. Microbiol., 50: 727-751. 1996 https://doi.org/10.1146/annurev.micro.50.1.727
  7. Henrichsen. J.: Bacterial surface translocation: a survey and a classification. Bacteriol. Rev., 36: 478-503, 1972
  8. Karunasagar, I., Pai, R. and Malath, G.R.: Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection. Aquaculture. 128: 203-209, 1994 https://doi.org/10.1016/0044-8486(94)90309-3
  9. Kirov. S.M.: Bacteria that express lateral flagella enable dissection of the multifunctional roles of flagella in pathogenesis. FEMS Microbol., 224: 151-159, 2003 https://doi.org/10.1016/S0378-1097(03)00445-2
  10. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature.227: 680-685. 1970 https://doi.org/10.1038/227680a0
  11. Lee, K.K. and Yii, K.C.: A comparison of three methods for assaying hydrophobicity of pathogenic vibrios. Lett. Appl. Microbiol., 23: 343-346, 1996 https://doi.org/10.1111/j.1472-765X.1996.tb00204.x
  12. Leifson, E.: Atlas of Bacterial Flagellation, Academic Press, New York, 1960
  13. Leong, T.S. and Wong, S.Y.: Environmental induced mortality of cultured grouper Epinephelus malabaricusinfected with high density of monogeneans and vibrios, In: Programme and Abstracts of the second symposium on diseases in Asian aquaculture (Phuket). Manila: Health Section of the Asian Fisheries Society. p37, 1993
  14. Liu, P.C., Chuang, W.H., and Lee. K.K.: Infectious gastroenteritis caused by Vibrio harveyi (V. carchariae) in cultured red drum, Sciaenops ocellatus. J. Appl. Ichthyol., 19: 59-61, 2003 https://doi.org/10.1046/j.1439-0426.2003.00356.x
  15. Liu, P.C., Lee, K.K., Yii, K.C., Kou, G.H. and Chen. S.N.: Isolation of Vibrio harveyi from diseased kuruma prawns Penaeus japonicus. curr. Microbiol., 33: 129-132. 1996 https://doi.org/10.1007/s002849900087
  16. McCarter. L.L.: Dual flagellar systems enable motility under different circumstances. J. Mol. Microbiol. Biotechnol., 7: 18-29. 2004 https://doi.org/10.1159/000077866
  17. Nealson. K.H. and Hastings, J.W.: Bacterial bioluminescence: Its control and ecological significance. Microbiol. Rev., 43: 496-518. 1979
  18. Nishimori. E., Hasegawa, O. Numata, T. and Wakabayashi, H.: Vibrio carchariae causes mass mortalities in Japanese abalone, Sulculus diversicolor supratexta. Fish Pathol., 33: 495-502,1998 https://doi.org/10.3147/jsfp.33.495
  19. Ortigosa, M., Garay, E and Pujalte, M.J.: Numerical taxonomy of Vibrionaceae isolated from oysters and seawater along an annual cycle. System. Appl. Microbiol., 17: 216-225, 1994 https://doi.org/10.1016/S0723-2020(11)80011-1
  20. Pedersen, K., Verdonck, L., Austin. B., Austin. D.A., Blanch, A.R., Grimont, P.A.D., Jofre. J., Koblavi, S., Larsen, J.L., Tiainen. T., Vigneulle, M. and Swings, J.: Taxonomic evidence that vibrio carchariae Grimes et al. 1985 is junior synonym of Vibrio harveyi (Johnson and Shunk 1936) Baumann et al. 1981. Int. J. Syst. Bacteriol., 48: 749-758, 1998 https://doi.org/10.1099/00207713-48-3-749
  21. Pizzutto, M. and Hirst, R.G.: Classification of isolates of vibria harveyi virulent to Penaeus monodon larvae by protein profile analysis and M13 DNA fingerprinting. Dis. Aquat. Org., 21: 61-68, 1995 https://doi.org/10.3354/dao021061
  22. Saeed. M.O.: Association of Vibrio harveyi with mortalities in cultured marine fish in Kuwait. Aquaculture, 136: 21-29, 1995 https://doi.org/10.1016/0044-8486(95)01045-9
  23. Soffientino, B., Gwaltney. T., Nelson, D.R., Specker, J.L., Mauel, M. and Gomez-Chiarri, M.: Infectious necrotizing enteritis and mortality caused by Vibrio carchariae in summer flounder Paralichthys dentatus during intensive culture. Dis. Aquat. Org., 38: 201-210, 1999 https://doi.org/10.3354/dao038201
  24. Song, Y.L. and Lee. S.P.: Characterization and ecological implication of luminous Vibrio harveyi isolated from tiger shrimp (Penaeus monodon). Bull. Inst. Zool. Academia Sinica. 32: 217-220, 1993
  25. Ulitzur, S. and Kessel. M.: Giant flagellar bundles of vibrio alginolyticus(NCMB 1803). Arch. Micriobiol., 94: 331-339.1975
  26. Wimpee, C.F., Nadeau, T.L. and Nealson, K.H.: Development of species-specific hybridization probes for marine luminous bacteria by using in vitro DNA amplification. Appl. Environ. Microbiol. 57: 1319-1324, 1991
  27. Won, K.M., Kim S.M. and Park S.I.: Characterization of Vibrio harveyi, the causal agent of Vibriosis in cultured marine fishes in Korea. J. Fish. Sci. Technol., 9(3): 123-128, 2006 https://doi.org/10.5657/fas.2006.9.3.123
  28. Yii, K.C., Yang, T.I. and Lee, K.K.: Isolation and Characterization of Vibrio carchariae, a the causative agent of gastroenteritis in the groupers, Epinephelus coioides. Curr. Microbiol., 35:109-115, 1997 https://doi.org/10.1007/s002849900221